Membrane Chromatography


By combining the functionality of resin-based columns, the flow rates of membranes, and single-use architecture, the Natrix® membrane technology enables a new level of downstream productivity and versatility. Natrix® single-use membrane chromatography technology can be utilized for initial capture steps all the way through polishing, and is adaptable to most ligand chemistries or affinity products.

Merck:/Freestyle/PS-Process-Solutions/BioP-Mfg-Biopharmaceutical-Manufacturing/downstream/natrix/natrix_proprietary_chart.png
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Merck:/Freestyle/PS-Process-Solutions/BioP-Mfg-Biopharmaceutical-Manufacturing/downstream/natrix/NatriFlo-HD-Q-pilot-device-small.jpgNatriFlo® HD-Q Membrane Adsorber

Membrane adsorber for single-use flow-through polishing applications. 

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Ordering Information 

Natrix® Membrane Chromatography Technology

How it Works

The Natrix® HD-Q membrane is a porous polyacrylamide hydrogel containing a high density of pendant quaternary ammonium (Q) binding groups physically reinforced by an inert fiber web. The interconnected pore structure (nominal pore size 0.4 μm) provides a large surface area for protein binding and high permeability. This unique combination of high density Q hydrogel chemistry, high definition protein separation and porosity produces a membrane with a very high binding capacity across a wide range of flow rates.

The Natrix® HD-Q membrane is composed of a porous, functionalized 3-dimensional hydrogel polymerized within and around a flixible fiber web support
NatriFlo
Flexible polymeric support web physically reniforces functional hydrogel
NatriFlo
Web is enclosed by and filled with porous functionalized hydrogel, establishing pore size


Merck:/Freestyle/PS-Process-Solutions/BioP-Mfg-Biopharmaceutical-Manufacturing/downstream/natrix/natrix-flow-smallv2.jpg

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High functional binding group density

Membranes featuring a hydrogel that is comprised of functional monomer. As a result, membranes are uniformly and densely saturated with binding sites.
Functional groups are readily accessible

Feed stream passes through interconnected pores (nominal pore size 0.4 μm) where every contact surface is loaded with functional binding groups. The advective mass transfer in the pore network enables high binding performance at much higher flow rates than diffusion based resins.
   
Multi-point molecular binding

The close proximity of binding sites allows for multi-point ionic bonding so that protein molecules can be bound by multiple charge points simultaneously. This results in a more robust binding even under difficult operating conditions with respect to pH, salt and buffer ions.
Single Use

Products are compact and single use, eliminating validation, cleaning, sanitization, and storage, enabling flexible downstream processing.


Key Advantages of Single-Use Chromatography


Reduce (60-80%) physical size and footprint

Eliminate large-scale chromatography columns
  • No column packing
  • No resin storage
  • No storing of packed columns
  • Reduced labor

Increase plant flexibility/modularity accommodating multi-product facilities (smaller formats)

Reduce buffer usage
(80% reduction in: water, chemicals for buffer, disposal)

Achieve required production scale capacity in a much smaller package

 
 
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