71341 pRSFDuet™-1 DNA - Novagen

71341
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      Overview

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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      71341-3
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          Plastic ampoule 10 μg
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          Description
          OverviewpRSFDuet™-1 is designed for the coexpression of two target proteins. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). The vector also carries the pRSF1030 replicon (also known as NTP1), lacI gene, and kanamycin resistance marker. This vector can be transformed into the same cell with pETDuet™-1, pACYCDuet™-1, and/or pCDFDuet™-1 for the coexpression of up to eight target proteins. ORFs inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. ORFs inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer.
          This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.




          This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
          Catalogue Number71341
          Brand Family Novagen®
          Application Data
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          Biological Information
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          Toxicological Information
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          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage ≤ -70°C
          Do not freeze Ok to freeze
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          Documentation

          pRSFDuet™-1 DNA - Novagen SDS

          Title

          Safety Data Sheet (SDS) 

          pRSFDuet™-1 DNA - Novagen Certificates of Analysis

          TitleLot Number
          71341

          Citations

          Title
        • Steven Raynard, Wendy Bussen and Patrick Sung. (2006) A double Holliday junction dissolvasome comprising BLM, topoisomerase III, and BLAP75. Journal of Biological Chemistry 281, 13861-13864.
        • Niraj H. Tolia and Leemor Joshua-Tor. (2006) Strategies for protein coexpression in Escherichia coli. Nature Methods 3, 55-64.
        • User Protocols

          Title
          TB340 Duet Vectors
          TB401 pRSF and pCDF Vectors

          Vector Map

          Title
          TB391 pRSFDuet™-1 Vector Map

          Vector Sequence

          Title
          pRSFDuet™-1 Vector Sequence