71339 pIEx™-7 Ek/LIC Vector Kit - Novagen

71339
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      Overview

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      71339-3
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          Plastic ampoule 20 rxn
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          Description
          OverviewThe pIEx™-7 Ek/LIC Vector is a ligation-independent cloning-prepared derivative of the pIEx-6 vector designed to allow rapid, high-level protein expression in insect cells without the time-consuming process of creating a recombinant baculovirus. This vector features the hr5 enhancer and the IE1 (immediate early) promoter to direct expression in insect cells and a minimal N-terminal His•Tag® coding sequence. This promoter/enhancer combination recruits endogenous insect cell transcription machinery, thereby avoiding baculovirus infection and the associated cytotoxic effects. The Ek/LIC cloning site is designed to allow the generation of fusion proteins with minimal vector-encoded sequence. An optional S•Tag™ coding sequence is present at the 3′ end of the MCS for generating a carboxy-terminal tag compatible with purification, detection and quantification (1).
          This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
          Catalogue Number71339
          Brand Family Novagen®
          SynonymsInsectDirect™ pIEx™-7 Ek/LIC Vector Kit
          References
          ReferencesKim, J.S. and Raines, R.T. (1993) Protein Science 2, 348–356.
          Product Information
          1 µgEk/LIC Vector
          25 unitsT4 DNA Polymerase, LIC-qualified
          50 µl25 mM EDTA
          40 µl25 mM dATP
          8 µlEk/LIC β-gal Control Insert
          100 µl100 mM DTT
          50 µl10X T4 DNA Polymerase Buffer
          1.5 mlNuclease-free Water
          5 × 2 mlSOC Medium
          10 µlTest Plasmid
          22 × 50 µlNovaBlue GigaSingles™ Competent Cells
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          pIEx™-7 Ek/LIC Vector Kit - Novagen SDS

          Title

          Safety Data Sheet (SDS) 

          pIEx™-7 Ek/LIC Vector Kit - Novagen Certificates of Analysis

          TitleLot Number
          71339

          References

          Reference overview
          Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348–356.

          Brochure

          Title
          Insect Direct!" Protein Expression & Purification System
          Radiance!" Multisystem Protein Expression

          Citations

          Title
        • Kathryn H. Loomis, et al. (2005) InsectDirectTM System: rapid, high-level protein expression and purication from insect cells. Journal of Structural and Functional Genomics 6, 189-194.
        • User Protocols

          Title
          TB163 Ek/LIC Cloning Kits

          Vector Map

          Title
          TB399VM pIEx-7 Ek/LIC Vector Map

          Vector Sequence

          Title
          pIEx-7 Ek/LIC Vector Sequence