71146 pETDuet™-1 DNA - Novagen

71146
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      71146-3
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          Plastic ampoule 10 μg
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          Description
          OverviewCoexpression of multiple target genes in E. coli is advantageous for studying protein complexes. Coexpression often achieves optimal yield, solubility, and activity and may protect individual subunits from degradation. The Duet vectors are T7 promoter expression vectors, each designed to coexpress two target proteins in E. coli. The Duet vectors carry compatible replicons and antibiotic resistance markers and may be used together in appropriate host strains to coexpress up to eight proteins. Certain combinations of Duet vectors and pET or pETcoco™ vectors are also compatible for coexpression. The capability of Duet vectors to be cotransformed, propagated, and induced for robust target protein coexpression makes them ideal for the analysis of protein complexes. The Duet vectors are designed with compatible replicons and drug resistance genes for effective propagation and maintenance of four plasmids in a single cell.
          pETDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS), each of which is preceded by a T7 promoter, lac operator and ribosome binding site (rbs). The vector also carries the pBR322-derived ColE1 replicon, lacI gene and ampicillin resistance gene. This vector can be transformed into the same cell with pACYCDuet™-1, pCDFDuet™-1, and pRSFDuet™-1 or pCOLADuet™-1 in the appropriate host strain for the coexpression of up to eight target genes. Genes inserted into MCS-1 can be sequenced using the pETUpstream Primer and DuetDOWN1 Primer. Genes inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer.




          This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
          This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
          Catalogue Number71146
          Brand Family Novagen®
          References
          Product Information
          Fusion tagHis•Tag, S•Tag
          Vector familyDuet pET bacterial prokaryotic
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          pETDuet™-1 DNA - Novagen SDS

          Title

          Safety Data Sheet (SDS) 

          pETDuet™-1 DNA - Novagen Certificates of Analysis

          TitleLot Number
          71146

          Citations

          Title
        • Ang E.L., et al. 2007. Federation of American Societies for Experimental Biology Journal 274, 928.
        • Tolbert, W.D., et al. 2007. Procedings of the National Academy of Science 104, 14592.
        • Zhao, K.-H., et al. 2007Procedings of the National Academy of Science 104, 14300.
        • Gina Boanca, G., et al. 2006. Uncoupling enzymatic and autoprocessing activities of H. pylori γ-glutamyltranspeptidase. Journal of Biological Chemistry 281, 19029.
        • Gengenbacher, M., et al. 2006. Journal of Biological Chemistry 281, 3633.
        • Sasaki, H.M., et al. 2006. Procedings of the National Academy of Science 103, 14744.
        • Niraj H. Tolia and Leemor Joshua-Tor. 2006. Strategies for protein coexpression in Escherichia coli. Nature Methods 3, 55.
        • Vicogne, J., et al. 2006. Procedings of the National Academy of Science 103, 14761.
        • Arnold, L.A., et al. 2005. Journal of Biological Chemistry 280, 43048-43055.
        • Chu, H.M.A., et al. 2005. European Molecular Biology Organization Journal 24, 3214.
        • Archa H. Fox, Charles S. Bond and Angus I. Lamond. (2005) P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in a RNA-dependent manner. Molecular Biology of the Cell 16, 5304-5315.
        • Dejana Mokranjac, et al. (2005) Role of Tim21 in mitochondrial translocation contact sites. Journal of Biological Chemistry 280, 23437-23440.
        • Lennart Randau, et al. (2005) The heteromeric Nanoarchaeum equitans splicing endonuclease cleaves noncanonical bulge-helix-bulge motifs of joined tRNA halves. Proceedings of the National Academy of Sciences (USA) 102, 17934-17939.
        • John Sergeant, et al. (2005) Composition and architecture of the Schizosaccharomyces pombe rad18 (Smc5-6) complex. Molecular and Cellular Biology 25, 172-184.
        • Martin Sichting, et al. (2005) Maintenance of structure and function of mitochondrial Hsp70 chaperones requires the chaperone Hep1. European Molecular Biology Organization Journal 24, 1046-1056.
        • Ronnie R. Wei, Peter K. Sorger and Stephen C. Harrison. (2005) Molecular organization of the Ndc80 complex, an essential kinetochore component. Proceedings of the National Academy of Sciences (USA) 102, 5363-5367.
        • Si-houy Lao-Sirieix and Stephen D. Bell. (2004) The heterodimeric primase of the hyperthermophilic archaeon Sulfolobus solfataricus possesses DNA and RNA primase, polymerase and 3'-terminal nucleotidyl transferase activity. Journal of Molecular Biology (in press 11/10/04), (in press).
        • Vladimir V. Lunin, et al. (2004) The structure of the MAPK scaffold, MP1, bound to Its partner, p14 A complex with a critical role in endosomal MAP kinase signaling. Journal of Biological Chemistry 279, 23422-23430.
        • Christian G. Noble, et al. (2004) Rna14-Rna15 assembly mediates the RNA-binding capability of Saccharomyces cerevisiae cleavage factor IA. Nucleic Acids Research 32, 3364-3375.
        • Yoshiko Ohgari, et al. (2004) Ferrochelatase consisting of wild-type and mutated subunits from patients with a dominant-inherited disease, erythropoietic protoporphyria, is an active but unstable dimer. Human Molecular Genetics in press 12/16/04,.
        • An X. Tran, et al. (2004) Periplasmic cleavage and modification of the 1-phosphate group of Helicobacter pylori lipid A. Journal of Biological Chemistry 279, 55780-55791.
        • Matthias Versele, et al. (2004) Protein-protein interactions governing septin heteropentamer assembly and septin filament organization in Saccharomyces cerevisiae. Molecular Biology of the Cell 15, 4568-4583.
        • Matthias Versele and Jeremy Thorner. (2004) Septin collar formation in budding yeast requires GTP binding and direct phosphorylation by the PAK, Cla4. Journal of Cell Biology 164, 701-715.
        • Benedykt Wladyka, Katarzyna Puzia and Adam Dubin. (2004) Efficient co-expression of a recombinant staphopain A and its inhibitor staphostatin A in Escherichia coli. Biochemical Journal.
        • User Protocols

          Title
          TB340 Duet Vectors

          Vector Map

          Title
          TB337VM pETDuet™-1 Vector Map