71464 pET-50b(+) DNA - Novagen

71464
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      Overview

      Replacement Information

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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      71464-3
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          Plastic ampoule 10 μg
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          Description
          OverviewThe pET-50b(+) vector is designed for cloning and high-level expression of target proteins fused with the 495 aa NusA (Nus•Tag™) protein that is cleavable with the human rhinovirus (HRV) 3C protease (1). The NusA protein has been identified as having the highest potential for solubility when a data base of more than 4000 E. coli proteins was subjected to solubility modeling (2,3). Greater than 85% of the expressed protein was soluble in tests with each of four different NusA fusion proteins (2). Cloning sites are also available for producing fusion proteins containing two cleavable N-terminal His•Tag® sequences for detection and purification. The additional N-terminal His•Tag sequence significantly enhances purification yields when utilizing His•Mag™ Agarose Beads for high throughput purification (4). The plasmid contains a strong T7lac promoter, optimized RBS, the coding sequence for the HRV 3C protease cleavage site (LeuGluValLeuPheGlnGlyPro) for N-terminal fusion tag removal and a multiple cloning site that encodes restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal S•Tag™ coding sequence is compatible with purification, detection, and quantification (5).

          The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




          This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
          This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
          Catalogue Number71464
          Brand Family Novagen®
          References
          References1. Walker, P.A., Leong L. E.-C., Ng, P.W.P., Tan, S.H.,Waller, S., Murphy, D. and Porter, A.G. (1994) Bio/Technology 12, 601-605.

          2. Harrison, R.G. (2000) inNovations 11, 4-7.

          3. Davis, G.D., Elisee, C., Newham, D.M. and Harrison, R.G. (1998) Biotechnol. Bioeng. 65, 382-388.

          4. Novy, R. and Drott, D. (2002) inNovations 14, 12-13.

          5. Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348-356.
          Product Information
          Fusion tagHis•Tag, Nus•Tag, S•Tag
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          pET-50b(+) DNA - Novagen SDS

          Title

          Safety Data Sheet (SDS) 

          pET-50b(+) DNA - Novagen Certificates of Analysis

          TitleLot Number
          71464

          References

          Reference overview
          1. Walker, P.A., Leong L. E.-C., Ng, P.W.P., Tan, S.H.,Waller, S., Murphy, D. and Porter, A.G. (1994) Bio/Technology 12, 601-605.

          2. Harrison, R.G. (2000) inNovations 11, 4-7.

          3. Davis, G.D., Elisee, C., Newham, D.M. and Harrison, R.G. (1998) Biotechnol. Bioeng. 65, 382-388.

          4. Novy, R. and Drott, D. (2002) inNovations 14, 12-13.

          5. Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348-356.

          Brochure

          Title
          The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression (EMD)

          User Protocols

          Title
          TB055 pET System Manual

          Vector Map

          Title
          TB418VM pET-50b(+) Vector Map