69016 pET-32b(+) DNA - Novagen

69016
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      Overview

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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      69016-3
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          Plastic ampoule 10 μg
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          Description
          OverviewThe pET-32b(+) vector is designed for cloning and high-level expression of peptide sequences fused with the 109aa Trx•Tag™ thioredoxin protein (1). Cloning sites are available for producing fusion proteins also containing cleavable His•Tag® and S•Tag™ sequences for detection and purification. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB122 ). The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.

          The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




          This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
          This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
          Catalogue Number69016
          Brand Family Novagen®
          References
          References1. LaVallie, E.R., DiBlasio, E.A., Kovacic, S., Grant, K.L., Schendel, P.F. and McCoy, J.M. (1993) Bio/Technology 11, 187–193.
          Product Information
          Vector familypET
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          pET-32b(+) DNA - Novagen SDS

          Title

          Safety Data Sheet (SDS) 

          pET-32b(+) DNA - Novagen Certificates of Analysis

          TitleLot Number
          69016

          References

          Reference overview
          1. LaVallie, E.R., DiBlasio, E.A., Kovacic, S., Grant, K.L., Schendel, P.F. and McCoy, J.M. (1993) Bio/Technology 11, 187–193.

          Brochure

          Title
          The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression (EMD)

          Citations

          Title
        • Cecilia Angelelli, et al. (2008) Differentiation-dependent lysine 4 acetylation enhances MEF2C binding to DNA in skeletal muscle cells. Nucleic Acids Research 36, 915-928.
        • Kristel Vercauteren, et al. (2006) PGC-1-related coactivator: immediate early expression and characterization of a CREB/NRF-1 binding domain associated with cytochrome c promoter occupancy and respiratory growth. Molecular and Cellular Biology 26, 7409-7419.
        • Patricia L. Herman, et al. (2005) A three component dicamba O-demethylase from Pseudomonas maltophilia, strain DI-6: Gene isolation, characterization, and heterologous expression. Journal of Biological Chemistry 280, 24759-24767.
        • Naoko Tsuneyoshi, et al. (2005) The functional and structural properties of MD-2 required for lipopolysaccharide binding are absent in MD-11. Journal of Immunology 174, 340-344.
        • Franklin E. Callahan, et al. (2004) Comparison of MIC-3 protein accumulation in response to root-knot nematode infection in cotton lines displaying a range of resistance levels. Journal of Cotton Science 8, 186-190.
        • Daniela Barilla, Barbara A. Lee and Nick J. Proudfoot. (2001) Cleavage/ polyadenylation factor IA associates with the carboxyl-terminal domain of RNA polymerase II in Saccharomyces cerevisiae. Procedings of the National Academy of Science 98, 445-450.
        • Elizabeth A. Walker, et al. (2001) Functional expression, characterization, and purification of the catalytic domain of human 11-β-hydroxysteroid dehygronase type I. Journal of Biological Chemistry 276, 21343-21350.
        • User Protocols

          Title
          TB055 pET System Manual

          Vector Map

          Title
          TB122VM pET-32a-c(+) Vector Map

          Vector Sequence

          Title
          pET-32b(+) Vector Sequence

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          Categories

          Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors