69663 pET-17b DNA - Novagen

69663
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      Overview

      Replacement Information

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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      69663-3
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          Plastic ampoule 10 μg
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          Description
          OverviewThe pET-17b vector carries an N-terminal 11aa T7•Tag® sequence followed by a region of useful cloning sites. Included in the multiple cloning region are dual BstX I sites, which allow efficient cloning using an asymmetric linker (1). Unique sites (except for the two BstX I sites) are shown on the vector map (TB047). Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map.

          The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




          This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
          This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
          Catalogue Number69663
          Brand Family Novagen®
          References
          References1. Seed, B. (1987) Nature 329, 840.
          Product Information
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          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
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          Supplemental Information
          Specifications

          Documentation

          pET-17b DNA - Novagen SDS

          Title

          Safety Data Sheet (SDS) 

          pET-17b DNA - Novagen Certificates of Analysis

          TitleLot Number
          69663

          References

          Reference overview
          1. Seed, B. (1987) Nature 329, 840.

          Citations

          Title
        • Heidi A. Erickson, Michelle D. Jund and Christopher A. Pennell. 2006. Protein Engineering Design and Selection 19, 37.
        • Aichberger, K.J., et al. 2005. Journal of Immunology 175, 1286.
        • Kim, Y., et al. 2005. Journal of Biological Chemistry 280, 23273.
        • Georg W. Mayr, Sabine Windhorst and Kirsten Hillemeier. 2005. Journal of Biological Chemistry 280, 13229.
        • Mori, S., et al. 2005. Journal of Biological Chemistry 280, 24104.
        • Suzuki, M., et al. 2005. Journal of Biological Chemistry 280, 21444.
        • Zhao, B., et al. 2005. Journal of Biological Chemistry 280, 11599.
        • James S. Parker, S. Mark Roe and David Barford. 2004. European Molecular Biology Organization Journal 23, 4727.
        • M. Schuster, M. L. Urbanowski and E. P. Greenberg. 2004. Procedings of the National Academy of Science 101, 15833.
        • Mazumder, B., et al. 2003. Cell 115, 187.
        • Shi, Z-Z., et al. 1996. Nature Genetics 14, 361.
        • User Protocols

          Title
          TB055 pET System Manual

          Vector Map

          Title
          TB047VM pET-17b Vector Map

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          Categories

          Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors