69660 pET-14b DNA - Novagen

69660
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      Overview

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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      69660-3
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          Plastic ampoule 10 μg
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          Description
          OverviewThe pET-14b vector carries an N-terminal His•Tag® sequence followed by a thrombin cleavage site and three cloning sites. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB044).

          The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.





          This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
          This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
          Catalogue Number69660
          Brand Family Novagen®
          References
          Product Information
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          pET-14b DNA - Novagen SDS

          Title

          Safety Data Sheet (SDS) 

          pET-14b DNA - Novagen Certificates of Analysis

          TitleLot Number
          69660

          Citations

          Title
        • Kutuzova, G.D., et al. 2006. Procedings of the National Academy of Science 103, 12377.
        • Meyn, M.A., et al. 2006. Journal of Biological Chemistry 281, 30907.
        • Chai, S.C., et al. 2005. Journal of Biological Chemistry 280, 9865.
        • Laetitia Fragnet, Emmanuel Kut and Denis Rasschaert. 2005. Journal of Biological Chemistry 280, 23502.
        • Mori, S., et al. 2005. Journal of Biological Chemistry 280, 24104.
        • Raux-Deery, E., et al. 2005. Journal of Biological Chemistry 280, 4713.
        • Snipes, J.A., et al. 2005. Journal of Pharmacology and Experimental Therapeutics 313, 668.
        • Yurchenko, V., et al. 2005. Journal of Biological Chemistry 280, 17013.
        • Bühling, F., et al. 2004. Clin. Diagn. Lab Immunol. 11, 131.
        • Gu, D., et al. 2004. Journal of Biological Chemistry 279, 31171.
        • Guo, S., et al. 2000. Nature 408, 366.
        • Pellegrini, L., et al. 2000. Nature 407, 1029.
        • Winfried Hausner and Michael Thomm. 1995. Journal of Biological Chemistry 270, 17649.
        • Laura M. McMurry and Stuart B. Levy. 1995. Journal of Biological Chemistry 270, 22752.
        • Tait, J.F., et al. 1995. Journal of Biological Chemistry 270, 21594.
        • User Protocols

          Title
          TB055 pET System Manual

          Vector Map

          Title
          TB044VM pET-14b Vector Map

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          Categories

          Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors