500554 mTOR Activator, MHY1485 - Calbiochem

500554
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical Formula
      C₁₇H₂₁N₇O₄

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      5.00554.0001
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          Glass bottle 10 mg
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          Description
          OverviewA cell-permeable triazine derivative that is shown to increase cellular mTOR Ser2448 and downstream substrate 4E-BP Thr37/46 phosphorylation level in rat liver Ac2F cells (1 to 2 µM for 1 h), affecting culture viability only at much higher concentrations (by 20% after 24 h 20 µM treatment). MHY1485 induced cellular LC3-II accumulation in Ac2F is reported to be a result of autophagy inhibition due to reduced fusion between autophagosomes and lysosomes. The mechanism of mTOR activation by MHY1485 is currently unknown.
          Catalogue Number500554
          Brand Family Calbiochem®
          Synonyms4,6-dimorpholino-N-(4-nitrophenyl)-1,3,5-triazin-2-amine, Autophagy Inhibitor III
          References
          ReferencesChoi, Y. J., et al. 2012. PLoS. One. 7, e43418.
          Product Information
          FormPale yellow solid
          Hill FormulaC₁₇H₂₁N₇O₄
          Chemical formulaC₁₇H₂₁N₇O₄
          ReversibleY
          Structure formula ImageStructure formula Image
          Applications
          ApplicationmTOR Activator, MHY1485, CAS Number: 326914-06-1, is a cell-permeable activator of mTOR that inhibits autophagy by suppressing the fusion between autophagosomes and lysosomes.
          Biological Information
          Primary Target IC<sub>50</sub>~2 µ
          Purity≥97% by HPLC
          Physicochemical Information
          Cell permeableY
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          References

          Reference overview
          Choi, Y. J., et al. 2012. PLoS. One. 7, e43418.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision10-May-2013 JSW
          Synonyms4,6-dimorpholino-N-(4-nitrophenyl)-1,3,5-triazin-2-amine, Autophagy Inhibitor III
          DescriptionA cell-permeable triazine derivative that is shown to increase cellular phosphorylation level of mTOR Ser2448 (20% and 42% above basal in 1 h, respectively, with 1 and 2 µM MHY1485) and downstream substrate 4E-BP Thr37/46 (29% and 34% above basal in 1 h, respectively, with 1 and 2 µM MHY1485) in rat liver Ac2F cells, affecting culture viability only at much higher concentrations (by 20% after 24 h 20 µM treatment). MHY1485 induced cellular LC3-II accumulation in Ac2F (117%, 150%, and 250% of vehicle control LC3-II/LC3-I ratio, respectively, with 0.5, 1, and 2 µM treatment for 6 h; 142% and 172% of control ratio, respectively, with 2 µM treatment for 1 h or 12 h) is reported to be a result of autophagy inhibition due to reduced fusion between autophagosomes and lysosomes as demonstrated by a reduction of starvation-induced AdGFP-LC3 and lysosome dye co-localization (27% and 32.7% above control co-localization in 6 h serum deprived cultures with or without 2 µM MHY1485; 21% above control co-localization with 2 µM MHY1485 treatment alone and without serum starvation). The mechanism of mTOR activation by MHY1485 is currently unknown.
          FormPale yellow solid
          Intert gas (Yes/No) Packaged under inert gas
          Chemical formulaC₁₇H₂₁N₇O₄
          Structure formulaStructure formula
          Purity≥97% by HPLC
          SolubilityDMSO (12.5 mg/ml)
          Storage +2°C to +8°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Toxicity Standard Handling
          ReferencesChoi, Y. J., et al. 2012. PLoS. One. 7, e43418.