605204 Thrombin, Immobilized, Human Plasma

605204
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      Overview

      Replacement Information

      Key Specifications Table

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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      605204-1ML
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          Glass bottle 1 ml
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          Description
          OverviewNative thrombin from human plasma, bound to agarose beads.
          Catalogue Number605204
          Brand Family Calbiochem®
          References
          ReferencesXiang, B., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 7412.
          Product Information
          FormSuspension
          FormulationIn 100 mM NaCl, 20 mM Tris-HCl, ≤0.1% sodium azide, pH 7.4.
          Applications
          Biological Information
          SourcePrepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Thrombin, Immobilized, Human Plasma SDS

          Title

          Safety Data Sheet (SDS) 

          Thrombin, Immobilized, Human Plasma Certificates of Analysis

          TitleLot Number
          605204

          References

          Reference overview
          Xiang, B., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 7412.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision19-May-2008 RFH
          DescriptionNative thrombin from human plasma, bound to agarose beads. Thrombin is a key enzyme in the coagulation cascade. Converts fibrinogen to fibrin and activates factor XIII, which cross-links and stabilizes the fibrin polymer.
          FormSuspension
          FormulationIn 100 mM NaCl, 20 mM Tris-HCl, ≤0.1% sodium azide, pH 7.4.
          Concentration Label Please refer to vial label for lot-specific concentration
          Recommended reaction conditions
          Guidelines for Fusion Protein Cleavage: Adapted from Xiang, et al (see references) Please note that this protocol is provided only as a general guide. Researchers should standardize this assay for their own specific needs and should consult published literature. 1. Immobilized thrombin can be used either with a column or in batch format. For use with batch method the gel may be centrifuged at 3000 rpm or less for separation of agarose from supernatant containing cleaved protein. Higher speeds will result in difficulty resuspending the agarose. 2. A rule of thumb wt/wt ratio to obtain efficient cleavage for most fusion proteins is 1:10 to 1:100 (thrombin:fusion protein) 3. Determine the amount of thrombin required to achieve desired cleavage. Add buffer to this amount of immobilized thrombin to form a slurry. Acceptable buffers include Tris-HCl, containing 150 mM NaCl, citrate buffer, or PBS; the optimal pH is 7.4. For column cleavage, wash the column 2–3 times with buffer prior to addition of sample. The protein should also be resuspended in the same buffer prior to cleavage. 4. Incubation: 25°C, generally overnight; time course including 2 h, 5 h, and 18 h is recommended; assess cleavage by SDS-PAGE. It may be necessary to check cleavage at intermittent intervals (e.g. every 30 min.). 5. For column cleavage, protein that migrates through the column may be monitored at 280 nm.
          SourcePrepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          Merck USA index14 9383
          ReferencesXiang, B., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 7412.