PF060 TdT, Calf Thymus

PF060
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      PF060-.155ML
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          Plastic ampoule .155 ml
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          Description
          OverviewNative TdT from calf thymus. Tdt is a DNA polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxy terminus of either double of single stranded DNA in a template-independent manner.
          Catalogue NumberPF060
          Brand Family Calbiochem®
          SynonymsTerminal Deoxynucleotidyl Transferase
          References
          ReferencesChang, L.M.S. and F.J. Bollum. 1986. Crit. Rev. Biochem. 21, 27.
          Deng, G. and R. Wu. 1983. Methods Enzymol. 100, 96.
          Michelson, A.M. and S.H. Orkin. 1982. J. Biol. Chem. 257, 14773.
          Roychoudhury, R. and R. Wu. 1980. Methods Enzymol. 65, 43.
          Nelson, T. and D. Brutlag. 1979. Methods Enzymol. 68, 41.
          Roychoudhury, R., et al. 1976. Nucleic Acids Res. 3, 101.
          Bollum, F.J. 1974. In The Enzymes, 3rd edition (ed. P.D. Boyer), Vol. 10, 145. Academic Press, New York.
          Product Information
          Unit of DefinitionOne unit is the amount of enzyme required to transfer 1 nmol of dAMP from dATP to the 3ʹ-OH terminus of d(A)50 in 60 min at 37°C.
          FormLiquid
          Formulation775 Units in 50 mM potassium phosphate buffer, 1 mM B-mercaptoethanol, 50% glycerol, pH 7.2.
          PreservativeNone
          Applications
          Key Applications DNA Labeling
          Application NotesDNA Labeling
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          TdT, Calf Thymus SDS

          Title

          Safety Data Sheet (SDS) 

          TdT, Calf Thymus Certificates of Analysis

          TitleLot Number
          PF060

          References

          Reference overview
          Chang, L.M.S. and F.J. Bollum. 1986. Crit. Rev. Biochem. 21, 27.
          Deng, G. and R. Wu. 1983. Methods Enzymol. 100, 96.
          Michelson, A.M. and S.H. Orkin. 1982. J. Biol. Chem. 257, 14773.
          Roychoudhury, R. and R. Wu. 1980. Methods Enzymol. 65, 43.
          Nelson, T. and D. Brutlag. 1979. Methods Enzymol. 68, 41.
          Roychoudhury, R., et al. 1976. Nucleic Acids Res. 3, 101.
          Bollum, F.J. 1974. In The Enzymes, 3rd edition (ed. P.D. Boyer), Vol. 10, 145. Academic Press, New York.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision16-September-2008 RFH
          SynonymsTerminal Deoxynucleotidyl Transferase
          ApplicationDNA Labeling
          DescriptionNative TdT from calf thymus. Tdt is a DNA polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxy terminus of either double of single stranded DNA in a template-independent manner. Although TdT preferentially adds deoxynucleotides to 3'-extensions, tailing onto 5' overhangs or blunt ended double strands of DNA can also be achieved. TdT can be used for labeling DNA fragments and vectors with homopolymer tails and/or with modified deoxynucleotides such as biotin-dNTPs, 32P-dNTPs, cordycepin-dNTPs, or ddNTP.
          BackgroundThe Calbiochem® Terminal deoxynucleotidyl Transferase (TdT) is a DNA polymerase isolated from calf thymus. TdT catalyzes the addition of deoxynucleotides to the 3'-hydroxyl termini of either double or single stranded DNA molecules in a template independent manner. Although TdT preferentially adds deoxynucleotides to 3'-extensions, tailing onto 5' overhangs or blunt ended double strands of DNA can also be achieved. TdT can be used for labeling DNA fragments and vectors with homopolymer tails and/or with modified deoxynucleotides such as biotin-dNTPs, 32P-dNTPs, cordycepin-dNTPs, or ddNTP.
          FormLiquid
          Formulation775 Units in 50 mM potassium phosphate buffer, 1 mM B-mercaptoethanol, 50% glycerol, pH 7.2.
          Unit definitionOne unit is the amount of enzyme required to transfer 1 nmol of dAMP from dATP to the 3ʹ-OH terminus of d(A)50 in 60 min at 37°C.
          PreservativeNone
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesChang, L.M.S. and F.J. Bollum. 1986. Crit. Rev. Biochem. 21, 27.
          Deng, G. and R. Wu. 1983. Methods Enzymol. 100, 96.
          Michelson, A.M. and S.H. Orkin. 1982. J. Biol. Chem. 257, 14773.
          Roychoudhury, R. and R. Wu. 1980. Methods Enzymol. 65, 43.
          Nelson, T. and D. Brutlag. 1979. Methods Enzymol. 68, 41.
          Roychoudhury, R., et al. 1976. Nucleic Acids Res. 3, 101.
          Bollum, F.J. 1974. In The Enzymes, 3rd edition (ed. P.D. Boyer), Vol. 10, 145. Academic Press, New York.