616407 TACE Substrate IV, Fluorogenic - Calbiochem

616407
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      616407-1MG
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          Glass bottle 1 mg
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          Description
          OverviewAn internally-quenched fluorogenic decapeptide substrate that is useful for continuous monitoring of TACE activity and high-throughput screening of TACE inhibitors (kcat = 21.6 s-1 and substrate concentration at half the Vmax, S0.5 = 342 µM). The substrate is cleaved at Ala-Val amide bond. Acts as a poor substrate for MMP-1, MMP-9, and MMP-13. Displays better solubility in aqueous buffers.
          Catalogue Number616407
          Brand Family Calbiochem®
          SynonymsTNF-α Convertase Enzyme Substrate IV, Fluorogenic, Abz-LAQAVRSSSR-Dpa, ADAM-17 Substrate IV, Fluorogenic
          References
          ReferencesJin, G., et al. 2002. Anal. Biochem. 302, 269.
          Product Information
          ATP CompetitiveN
          FormYellow lyophilized solid
          FormulationSupplied as a trifluoroacetate salt.
          Hill FormulaC₅₉H₉₃N₂₃O₂₀
          Chemical formulaC₅₉H₉₃N₂₃O₂₀
          ReversibleN
          Applications
          Biological Information
          Primary TargetFluorogenic decapeptide substrate that is useful for continuous monitoring of TACE activity
          Primary Target IC<sub>50</sub>kcat = 21.6 s-1 and substrate concentration at half the Vmax, S0.5 = 342 µM
          Purity≥95% by HPLC
          Physicochemical Information
          Cell permeableN
          Emission max.
          Excitation max.
          Peptide SequenceAbz-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-Dpa
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Protect from Moisture Protect from moisture
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          TACE Substrate IV, Fluorogenic - Calbiochem SDS

          Title

          Safety Data Sheet (SDS) 

          TACE Substrate IV, Fluorogenic - Calbiochem Certificates of Analysis

          TitleLot Number
          616407

          References

          Reference overview
          Jin, G., et al. 2002. Anal. Biochem. 302, 269.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision17-December-2018 JSW
          SynonymsTNF-α Convertase Enzyme Substrate IV, Fluorogenic, Abz-LAQAVRSSSR-Dpa, ADAM-17 Substrate IV, Fluorogenic
          DescriptionAn internally quenched, fluorogenic, 10-amino acid peptide substrate that is useful for monitoring TNF-α Convertase Enzyme (TACE) activity by a continuous fluorometric assay. Also useful for screening TACE inhibitors (kcat = 21.6 s-1 when the substrate concentration is half the Vmax, S0.5 = 342 µM). The substrate is cleaved at the Ala-Val amide bond. The substrate is not substantially cleaved by MMP-1, MMP-9, or MMP-13. Displays improved solubility in aqueous buffers. Excitation max: ~320 nm; Emission max: ~420 nm.
          FormYellow lyophilized solid
          FormulationSupplied as a trifluoroacetate salt.
          Recommended reaction conditions50 mM Tris-HCl, pH 7.4, 25 mM NaCl, 4% glycerol.
          Chemical formulaC₅₉H₉₃N₂₃O₂₀
          Peptide SequenceAbz-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-Dpa
          Purity≥95% by HPLC
          SolubilityDMSO (5 mg/ml)
          Storage +2°C to +8°C
          Protect from moisture
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
          Toxicity Standard Handling
          ReferencesJin, G., et al. 2002. Anal. Biochem. 302, 269.