189730 Streptavidin

189730
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      189730-10MG
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          Plastic ampoule 10 mg
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          189730-1MG
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          Stocked 
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          Available
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              Plastic ampoule 1 mg
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              Description
              OverviewA high-affinity biotin binding protein.
              Catalogue Number189730
              Brand Family Calbiochem®
              SynonymsAvidin, Streptomyces avidinii
              References
              ReferencesEberle, J. and Seibl, R. 1992. J. Virol. Methods 40, 347.
              Gudibande, S.R., et al. 1992. Mol. Cell. Probes. 6, 495.
              Nesbitt, S.A. and Horton, M.A. 1992. Anal. Biochem. 206, 267.
              Roth, J., et al. 1992. Histochemistry 98, 229.
              Sano, T., et al. 1992. Science 258, 120.
              Scouten, W.H. and Konecny, P. 1992. Anal. Biochem. 205, 313.
              Srivastava, P., et al. 1992. Cytometry 13, 711.
              Product Information
              CAS number9013-20-1
              Unit of DefinitionOne unit is defined as the amount of streptavidin required to bind 1.0 µg of D-Biotin at pH 7.0.
              FormLyophilized solid
              Applications
              Biological Information
              Specific Activity≥10 units/mg protein
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Ambient Temperature Only
              Toxicity Standard Handling
              Storage -20°C
              Protect from Moisture Protect from moisture
              Do not freeze Ok to freeze
              Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Aqueous stock solutions are stable for 6 months at 4°C and for up to 2 years at -20°C.
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              SDS

              Title

              Safety Data Sheet (SDS) 

              Certificates of Analysis

              TitleLot Number
              189730

              References

              Reference overview
              Eberle, J. and Seibl, R. 1992. J. Virol. Methods 40, 347.
              Gudibande, S.R., et al. 1992. Mol. Cell. Probes. 6, 495.
              Nesbitt, S.A. and Horton, M.A. 1992. Anal. Biochem. 206, 267.
              Roth, J., et al. 1992. Histochemistry 98, 229.
              Sano, T., et al. 1992. Science 258, 120.
              Scouten, W.H. and Konecny, P. 1992. Anal. Biochem. 205, 313.
              Srivastava, P., et al. 1992. Cytometry 13, 711.

              Brochure

              Title
              Antibody Sourcebook!" Second Edition
              Bulk Product Guide
              Protein Modifications Tools
              Data Sheet

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision17-April-2008 RFH
              SynonymsAvidin, Streptomyces avidinii
              DescriptionA high affinity biotin binding protein (Kd approximately 10-15) that is stable over wide pH and temperature ranges. Each molecule of streptavidin binds 4 molecules of biotin. Streptavidin lacks the carbohydrate side chains present on avidin and has an isoelectric point nearer to neutrality (pI of 5.5-6.5 versus 10 for avidin). Consequently, streptavidin frequently exhibits lower levels of non-specific binding than avidin. The extinction coefficient of a 1% solution at 280 nm is 32.
              FormLyophilized solid
              CAS number9013-20-1
              Specific activity≥10 units/mg protein
              Unit definitionOne unit is defined as the amount of streptavidin required to bind 1.0 µg of D-Biotin at pH 7.0.
              SolubilityReconstitute in H₂O or 1 mM NaHCO₃, pH 9.0 at twice the final concentration and then dilute with an equal volume of 2X buffer.
              Storage -20°C
              Protect from moisture
              Do Not Freeze Ok to freeze
              Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Aqueous stock solutions are stable for 6 months at 4°C and for up to 2 years at -20°C.
              Toxicity Standard Handling
              ReferencesEberle, J. and Seibl, R. 1992. J. Virol. Methods 40, 347.
              Gudibande, S.R., et al. 1992. Mol. Cell. Probes. 6, 495.
              Nesbitt, S.A. and Horton, M.A. 1992. Anal. Biochem. 206, 267.
              Roth, J., et al. 1992. Histochemistry 98, 229.
              Sano, T., et al. 1992. Science 258, 120.
              Scouten, W.H. and Konecny, P. 1992. Anal. Biochem. 205, 313.
              Srivastava, P., et al. 1992. Cytometry 13, 711.