560000 SPUTOLYSIN® Reagent - CAS 578517 - Calbiochem

560000
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      Overview

      Replacement Information

      Key Specifications Table

      CAS #
      3483-12-3

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      560000-10ML
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          Glass bottle 10 ml
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          560000-1SET
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              Glass bottle 1 set
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              Description
              OverviewConcentrate of Dithiothreitol (DTT; Cat. No. 233155) in phosphate buffer, pH 6.5-7.5. Intended for the isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum. Each vial is sufficient to make 100 ml of working solution.
              Note: 1 set = 20 x 10 ml.
              Catalogue Number560000
              Brand Family Calbiochem®
              References
              ReferencesHirsch, S.R., et al. 1969. J. Lab. Clin. Med. 74, 346.
              Shah, R.R. and Dye, W.E. 1966. Am. Rev. Respir. Dis. 94, 454.
              Dixon, J.M.S. and Miller, D.C. 1965 (November). Lancet p. 1046.
              Cleland, W.W. 1964. Biochemistry 3, 480.
              May, J.R. 1953 (September). Lancet p. 535.
              Product Information
              CAS number3483-12-3
              FormLiquid
              Formulation20 vials each containing 10 ml of concentrated DTT OR 1 vial containing 10 ml of concentrated DTT.
              Applications
              Biological Information
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              RTECSEK1610000
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Ambient Temperature Only
              Toxicity Harmful
              Storage +15°C to +30°C
              Protect from Light Protect from light
              Do not freeze Ok to freeze
              Special InstructionsOnce a vial is opened the unused concentrate should be purged with an inert gas (e.g. N₂) and refrigerated (4°C). Diluted solutions, stored aseptically under an inert gas, are stable for up to 48 h at 4°C.
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              SPUTOLYSIN® Reagent - CAS 578517 - Calbiochem SDS

              Title

              Safety Data Sheet (SDS) 

              SPUTOLYSIN® Reagent - CAS 578517 - Calbiochem Certificates of Analysis

              TitleLot Number
              560000

              References

              Reference overview
              Hirsch, S.R., et al. 1969. J. Lab. Clin. Med. 74, 346.
              Shah, R.R. and Dye, W.E. 1966. Am. Rev. Respir. Dis. 94, 454.
              Dixon, J.M.S. and Miller, D.C. 1965 (November). Lancet p. 1046.
              Cleland, W.W. 1964. Biochemistry 3, 480.
              May, J.R. 1953 (September). Lancet p. 535.

              Citations

              Title
            • I.S. Woolhouse, D.L. Bayley and R.A. Stockley. (2002) Effect of Sputum Processing with Dithiothreitol on the Detectino of Inflammatory Mediators in Chronic Bronchitis and Bronchiectasis. Thorax 57, 667-671.
            • Data Sheet

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision28-April-2008 RFH
              DescriptionA concentrate of dithiothreitol (DTT) in phosphate buffer. Useful for the isolation of pathogenic or saprophytic bacteria, fungi, yeast, and epithelial cells from sputum and other mucoid fluids.
              FormLiquid
              Formulation20 vials each containing 10 ml of concentrated DTT OR 1 vial containing 10 ml of concentrated DTT.
              Recommended reaction conditionsA. To prepare sputum samples: 1. In a screw-cap centrifuge tube, overlay the sputum sample with an equal volume of the diluted SPUTOLYSIN® Reagent. 2. Suspend the sputum by mixing on a vortex mixer for 30 s. 3. Allow the suspended mixture to stand at room temperature for 15 min. 4. Centrifuge the mixture for 5 min at 1500 rpm. Discard the supernatant. B. To prepare cultures for acid-fast bacilli: 1. Suspend the pellet from Step A(4) in 5 to 10 ml of 1% sodium hydroxide by mixing on a vortex mixer for 1 min. 2. Centrifuge the mixture for 15 min at 3000 rpm. Discard the supernatant. 3. Rinse the pellet twice in 10 ml of sterile normal saline to remove the residual sodium hydroxide. 4. Resuspend the pellet in 0.5 ml of the diluted SPUTOLYSIN® Reagent.
              CAS number3483-12-3
              RTECSEK1610000
              SolubilityDilute the contents of one vial to 100 ml with sterile distilled water. Swirl gently to dissolve any crystals. If the diluted material appears cloudy, the solution may be filtered to remove any insoluble particles. The diluted solution is ~6.5 mM dithiothreitol in 100 mM phosphate buffer, pH 7.0.
              Storage +15°C to +30°C
              Protect from light
              Do Not Freeze Ok to freeze
              Special InstructionsOnce a vial is opened the unused concentrate should be purged with an inert gas (e.g. N₂) and refrigerated (4°C). Diluted solutions, stored aseptically under an inert gas, are stable for up to 48 h at 4°C.
              Toxicity Harmful
              ReferencesHirsch, S.R., et al. 1969. J. Lab. Clin. Med. 74, 346.
              Shah, R.R. and Dye, W.E. 1966. Am. Rev. Respir. Dis. 94, 454.
              Dixon, J.M.S. and Miller, D.C. 1965 (November). Lancet p. 1046.
              Cleland, W.W. 1964. Biochemistry 3, 480.
              May, J.R. 1953 (September). Lancet p. 535.
              Citation
            • I.S. Woolhouse, D.L. Bayley and R.A. Stockley. (2002) Effect of Sputum Processing with Dithiothreitol on the Detectino of Inflammatory Mediators in Chronic Bronchitis and Bronchiectasis. Thorax 57, 667-671.