IP05 Protein G Plus/Protein A Agarose Suspension

IP05
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      IP05-1.5ML
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          Plastic ampoule 1.5 ml
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          Description
          OverviewDesigned for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification.
          Catalogue NumberIP05
          Brand Family Calbiochem®
          References
          Product Information
          FormLiquid slurry
          Formulation33% slurry in PBS.
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoprecipitation
          Application NotesImmunoprecipitation (see comments)
          Application CommentsAgarose solution is supplied ready to use. Protein G Plus/Protein A Agarose immunoprecipitation reagent is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl of solution per µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Protein G Plus/Protein A Agarose Suspension SDS

          Title

          Safety Data Sheet (SDS) 

          Protein G Plus/Protein A Agarose Suspension Certificates of Analysis

          TitleLot Number
          IP05

          Citations

          Title
        • Ellen J. Tisdale and Cristina R. Artalejo. (2006) Src-dependent a protein kinase C(aPKC) λ tyrosine phosphorylation is required for aPKCl/λ association with Rab2 and glyceraldehyde-3-phosphate dehydrogenase on pre-golgi intermediates. Journal of Biological Chemistry 281, 8436-8442.
        • Catherine S. Chew, et al. (2005) Drebrin E2 is differentially expressed and phosphorylated in parietal cells in the gastric mucosa. American Journal of Physiology Gastrointestinal and Liver Physiology 289, G320-G331.
        • Ellen J. Tisdale, Carmen Kelly and Cristina R. Artalejo. (2004) Glyceraldehyde-3-phosphate dehydrogenase interacts with Rab2 and plays an essential role in endoplasmic reticulum to golgi transport exclusive of its glycolytic activity. Journal of Biological Chemistry 279, 54046-54052.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision13-August-2008 JSW
          ApplicationImmunoprecipitation (see comments)
          DescriptionProtein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.
          BackgroundProtein G PLUS/Protein A-Agarose mixture provides superior performance for binding of most species and subclasses of immunoglobulins. For use with rat IgM antibodies, use with goat second-step antibodies.
          FormLiquid slurry
          Formulation33% slurry in PBS.
          Preservative≤0.1% sodium azide
          CommentsAgarose solution is supplied ready to use. Protein G Plus/Protein A Agarose immunoprecipitation reagent is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl of solution per µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          Citation
        • Ellen J. Tisdale and Cristina R. Artalejo. (2006) Src-dependent a protein kinase C(aPKC) λ tyrosine phosphorylation is required for aPKCl/λ association with Rab2 and glyceraldehyde-3-phosphate dehydrogenase on pre-golgi intermediates. Journal of Biological Chemistry 281, 8436-8442.
        • Catherine S. Chew, et al. (2005) Drebrin E2 is differentially expressed and phosphorylated in parietal cells in the gastric mucosa. American Journal of Physiology Gastrointestinal and Liver Physiology 289, G320-G331.
        • Ellen J. Tisdale, Carmen Kelly and Cristina R. Artalejo. (2004) Glyceraldehyde-3-phosphate dehydrogenase interacts with Rab2 and plays an essential role in endoplasmic reticulum to golgi transport exclusive of its glycolytic activity. Journal of Biological Chemistry 279, 54046-54052.
        • Related Products & Applications

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          Categories

          Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > Agarose Beads for IP & Antibody Purification
          Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > Agarose Beads for IP & Antibody Purification
          Life Science Research > Protein Sample Preparation > Protein Purification > Agarose Bead Affinity Purification > Agarose Beads for IP & Antibody Purification