203195 | Protein A, Biotin Conjugate

203195
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      203195-2MG
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          Plastic ampoule 2 mg
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          Description
          OverviewNative protein A conjugated to biotin.
          Catalogue Number203195
          Brand Family Calbiochem®
          References
          Product Information
          FormLiquid
          FormulationIn 10 mM PBS, 1% BSA, pH 7.6.
          Preservative≤0.1% sodium azide
          Applications
          Application NotesSolid Phase Immunoassay (1:10,000)
          Application CommentsThe protein A: biotin molar ratio is determined on a lot-specific basis. Binding strength is as follows: strongly reactive, guinea pig, human IgG1, IgG2, and IgG4, and rabbit; weakly reactive: bovine, human IgA, IgM, and IgE, and mouse; nonreactive: goat, horse, human IgD and IgG3, rat, and sheep. o-phenylenediamine, 1.0 mg/ml in citrate buffer, pH 4.5, 0.1% urea peroxide at 25°C, was used as substrate for testing biotin-Protein A and avidin peroxidase functionality. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          PurityA single band at MW 50,000 by SDS-PAGE
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          203195

          Brochure

          Title
          Antibody Sourcebook!" Second Edition
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision27-June-2007 RFH
          ApplicationSolid Phase Immunoassay (1:10,000)
          DescriptionNative protein from Straphylococcus Aureus, conjugated to biotin. Recognizes the Fc portions of immunoglobulins of various species.
          BackgroundLong-chain NHS-Biotin-labeled Protein A is an extremely useful reagent because of its high binding affinity for avidin, streptavidin and avidin- or streptavidin-labeled compounds. Protein A is the universal secondary antibody because it binds to the Fc portions of most immunoglobulin classes. It is a versatile and economic means to isolate, detect and purify antibodies produced by many animals. It is especially useful in immunohistochemical studies because, unlike secondary antibodies, it does not bind to cell Fc receptors, and therefore will not give high background reactions.
          FormLiquid
          FormulationIn 10 mM PBS, 1% BSA, pH 7.6.
          Concentration Label Please refer to vial label for lot-specific concentration
          PurityA single band at MW 50,000 by SDS-PAGE
          Preservative≤0.1% sodium azide
          CommentsThe protein A: biotin molar ratio is determined on a lot-specific basis. Binding strength is as follows: strongly reactive, guinea pig, human IgG1, IgG2, and IgG4, and rabbit; weakly reactive: bovine, human IgA, IgM, and IgE, and mouse; nonreactive: goat, horse, human IgD and IgG3, rat, and sheep. o-phenylenediamine, 1.0 mg/ml in citrate buffer, pH 4.5, 0.1% urea peroxide at 25°C, was used as substrate for testing biotin-Protein A and avidin peroxidase functionality. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling