SCM130 PluriSTEM™ Human ES/iPS Cell Medium

SCM130
500 ml  
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      Embryo Culture, Cult
      Description
      Catalogue NumberSCM130
      Trade Name
      • PluriSTEM
      DescriptionPluriSTEM™ Human ES/iPS Cell Medium
      OverviewPluriSTEM™ has been extensively tested and proven to possess the following characteristics for Human ES/iPS cell culture:

      • Supports the culture of pluripotent human ES/iPS cells for greater than 30 passages.
      • Eliminates the requirement to feed cells over the weekend. Take the weekend off.
      • Eliminates the requirement of daily feeding (i.e. Monday, Wednesday, Friday media exchanges).
      • Simple and easy transition of cells to PluriSTEM™ from feeder-based and feeder-free culture systems.
      • Enables superior single cell culture, passage and selection of human pluripotent clones.
      • Superior freeze-thaw cell viability and recovery.
      • Supports suspension culture.

      View our Application Note! (See the Data!)
      Background InformationEMD Millipore has developed a complete, defined and serum-free medium formulation for the feeder-free culture of human ES and iPS cells. PluriSTEM™ Human ES/iPS Cell (Cat. No. SCM130) is a small molecule based medium that enables weekend-free culture of human pluripotent stem cells and allows for media exchanges every other day without compromising the morphology or long term functionality of pluripotent stem cells. Pluripotent cells maintained in this moderate feeding regiment exhibited high cell health with minimal spontaneous differentiation,; expressed high levels of pluripotency markers (NANOG, OCT3/4, SOX-2, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81), retained differentiation potential and possessed a normal karyotype. PluriSTEM™ Human ES/iPS Medium is provided as a stand-alone 500 mL bottle that is ready-to-use and does not require additional supplementation. Cells proliferate faster in PluriSTEM™ as compared to the same cells plated in other serum-free and feeder-free media systems; a typical 4-6 day passage is expected for most pluripotent cell lines.
      References
      Product Information
      Applications
      ApplicationDefined small molecule based serum-free medium that enables feeder-free culture of human ES/iPS cells and allows for media exchanges every other day without compromising the morphology or long term functionality of human pluripotent stem cells.
      Key Applications
      • Embryo Culture
      • Stem Cell Culture
      Biological Information
      Media FormLiquid
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEach lot of PluriSTEM™ Human ES/iPS Medium is rigorously quality control tested for the ability to sustain undifferentiated human ES cells for greater than 3 passages.
      pH= 7.2-7.4
      Osmolality= 315-345
      Appearance= Red Clear Liquid
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsPluriSTEM™ Human ES/iPS Medium is provided frozen and can be stored at -20°C until the expiration date on product label. Before use, thaw PluriSTEM™ at room temperature or overnight at 2-8°C. Do not thaw at 37°C. If desired, PluriSTEM™ may be aseptically dispensed into working aliquots and stored at -20°C. Use aliquots within expiration date as indicated on label. Thawed aliquots may be stored at 2 – 8°C for up to 2 weeks. Do not refreeze aliquots after thawing. Before use, warm working aliquots to room temperature. Do not warm the medium in a 37°C water bath.
      Packaging Information
      Material Size500 ml
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Human pluripotent stem cells on artificial microenvironments: a high content perspective.
      Viswanathan, P; Gaskell, T; Moens, N; Culley, OJ; Hansen, D; Gervasio, MK; Yeap, YJ; Danovi, D
      Frontiers in pharmacology  5  150  2014

      Show Abstract
      25071572 25071572
      Doxycycline enhances survival and self-renewal of human pluripotent stem cells.
      Chang, MY; Rhee, YH; Yi, SH; Lee, SJ; Kim, RK; Kim, H; Park, CH; Lee, SH
      Stem cell reports  3  353-64  2014

      Show Abstract
      25254347 25254347
      Excision of viral reprogramming cassettes by Cre protein transduction enables rapid, robust and efficient derivation of transgene-free human induced pluripotent stem cells.
      Kadari, Asifiqbal, et al.
      Stem Cell Res Ther, 5: 47 (2014)  2014

      Show Abstract
      24713299 24713299

      Brochure

      Title
      Hallmarks of Aging (EMD)
      Simplifying the iPSC Workflow

      Technical Info

      Title
      Neural Induction Medium Efficiently Generates Multipotent Neural Progenitors from Human iPS Cells
      Single transfection of a synthetic polycistronic self-replicative RNA yields high numbers of transgene-free human iPSCs

      Posters

      Title
      A serum-free and feeder-free defined medium that enables weekend-free culture of undifferentiated human pluripotent stem cells

      User Guides

      Title
      PluriSTEM™ Human ES/iPS Medium