71296 PhosphoSafe™ Extraction Reagent

71296
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      71296-3
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          Plastic ampoule 25 ml
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          71296-4
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              Glass bottle 125 ml
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              Description
              OverviewPhosphoSafe™ Extraction Buffer efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state. This reagent contains the same formula as CytoBuster™ Protein Extraction Reagent (Trim 2001), but also includes four phosphatase inhibitors: sodium fluoride, sodium vanadate, β-glycerophosphate, and sodium pyrophosphate. PhosphoSafe Extraction Reagent is compatible with kinase assays, protein interaction analysis, and other applications. This reagent is provided in convenient 25-ml bottles (1 bottle for 71296-3, 5 bottles for 71296-4).


              Catalogue Number71296
              Brand Family Novagen®
              Features and benefits
              • Phosphorylation state preserved during extraction
              • Compatible with kinase assays
              References
              ReferencesTrim, J.E. and Sawyer, D.L. 2001. inNovations 12, 5.
              Product Information
              Applications
              Biological Information
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Dry Ice Only
              Toxicity Toxic
              Storage ≤ -70°C
              Do not freeze Ok to freeze
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              PhosphoSafe™ Extraction Reagent SDS

              Title

              Safety Data Sheet (SDS) 

              PhosphoSafe™ Extraction Reagent Certificates of Analysis

              TitleLot Number
              71296

              References

              Reference overview
              Trim, J.E. and Sawyer, D.L. 2001. inNovations 12, 5.

              Brochure

              Title
              Protein Purification and Preparation

              Technical Info

              Title
              Western Blotting Quick Reference Product Guide

              Citations

              Title
            • Yanxia Liu, et al. (2007) A genomic screen for activators of the antioxidant response element. Procedings of the National Academy of Science 104, 5205-5210.
            • Elena Martinelli, et al. (2007) HIV-1 gp120 inhibits TLR9-mediated activation and IFN-α secretion in plasmacytoid dendritic cells. Procedings of the National Academy of Science 104, 3396-3401.
            • Dagmar Pommereit and Fred S. Wouters. (2007) An NGF-induced Exo70-TC10 complex locally antagonises Cdc42-mediated activation of N-WASP to modulate neurite outgrowth. Journal of Cell Science 120, 2694-2705.
            • Aicha Saadane, et al. (2007) Parthenolide inhibits IκB kinase, NF-κB activation, and inflammatory response in cystic fibrosis cells and mice. American Journal of Respiratory Cell and Molecular Biology 36, 728-736.
            • Jeremy O. Jones and Ann M. Arvin. (2006) Inhibition of the NF-κB pathway by Varicella-Zoster virus in vitro and in human epidermal cells in vivo. Journal of Virology 80, 5113-5124.
            • Jeremy O. Jones and Ann M. Arvin. (2006) Inhibition of the NF-κB pathway by varicella-zoster virus in vitro and in human epidermal cells in vivo. Journal of Virology 80, 5113-5124.
            • Marisol Quintero, et al. (2006) Mitochondria as signaling organelles in the vascular endothelium. Proceedings of the National Academy of Sciences (USA) 103, 5379-5384.
            • Phuong Thi Nguyen Sarkis, et al. (2006) STAT1-independent cell type-specific regulation of antiviral APOBEC3G by IFN-α. Journal of Immunology 177, 4530-4540.
            • Anthony R. White, et al. (2006) Degradation of the alzheimer disease amyloid -peptide by metal-dependent up-regulation of metalloprotease activity. journal of Biological Chemistry 281, 17670-17680.
            • Ralf Gutzmer, et al. (2005) Histamine H4 receptor stimulation suppresses IL-12p70 production and mediates chemotaxis in human monocyte-derived dendritic cells. Journal of Immunology 5224-5232.
            • Mauro Cesar Isoldi, et al. (2005) Rhabdomeric phototransduction initiated by the vertebrate photopigment melanopsin. Procedings of the National Academy of Science 102, 1217-1221.
            • Elad Katz, et al. (2005) MMTV Env encodes an ITAM responsible for transformation of mammary epithelial cells in three-dimensional culture. Journal of Experimental Medicine 201, 431-439.
            • Hiroki Kuroda, et al. (2005) Default neural induction: neuralization of dissociated Xenopus cells is mediated by Ras/MAPK activation. Genes and Development 19, 1022-1027.
            • Markus Utech, et al. (2005) Mechanism of IFN-γ-Induced Endocytosis of Tight Junction Proteins: Mysoin II-Dependent Vacuolarization of the Apical Plasma Membrane. Molecular Biology of the Cell 16, 5040-5052.
            • User Protocols

              Title
              TB402 PhosphoSafe™ Extraction Reagent