577815 PhosphoDetect™ Anti-Tau (pSer³⁹⁶) Rabbit pAb

577815
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      577815-10T
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      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
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      Available
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          Plastic ampoule 10 t
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          Description
          OverviewRecognizes the ~45-68 kDa Tau protein phosphorylated at Ser396.
          Catalogue Number577815
          Brand Family Calbiochem®
          Application Data
          Detection of human tau, phosphorylated at Ser396, by immunoblotting. Samples: Whole cell lysate from control cells with exogenously added human recombinant tau left untreated (lane 1) or treated with GSK-3β (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Tau (pSer396) Rabbit pAb (Cat. No. 577815) (1:1000) pre-incubated without peptide (lanes 1, 2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphoserine containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
          References
          Referencesdel C. Alonso, A., et al. 2001. J. Biol. Chem. 276, 37967.
          Bennecib, M., et al. 2001. FEBS Lett. 490, 15.
          Sawamura, N., et al. 2001. J. Biol. Chem. 276, 10314.
          Tomidokoro, Y., et al. 2001. Neurosci. Lett. 299, 169.
          Bennecib, M., et al. 2000. FEBS Lett. 485, 87.
          Hashiguchi, M., et al. 2000. J. Biol. Chem. 275, 25247.
          Jenkins, S.M., et al. 2000. Biochem. J. 345, 263.
          Maas, T., et al., 2000. J. Biol. Chem. 275, 15733.
          Takahashi, M., et al. 2000. Brain. Res. 857, 193.
          Tesseur, I., et al. 2000. Am. J. Pathol. 156, 951.
          Schneider, A., et al. 1999. Biochemistry 38, 3549.
          Sengupta, A., et al. 1998. Arch. Biochem. Biophys. 357, 299.
          Wang, J.G., et al. 1998. FEBS Lett. 436, 28.
          Kopke, E., et al. 1993. J. Biol. Chem. 268, 24374.
          Arigada, P.A., et al. 1992. Neurology 42, 631.
          Drubin, D.G. and Kirschner, M.W. 1986. J. Cell. Biol. 103, 2739.
          Product Information
          FormLiquid
          FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.2-7.4.
          Positive controlRecombinant human tau treated with GSK-3β
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Application NotesImmunoblotting (1:1000)
          Application CommentsWhen used at a 1:1000 dilution, there is sufficient antibody for 10 immunoblots at 10 ml/blot. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁹⁶ phosphorylation site of human Tau
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          PhosphoDetect™ Anti-Tau (pSer³⁹⁶) Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          PhosphoDetect™ Anti-Tau (pSer³⁹⁶) Rabbit pAb Certificates of Analysis

          TitleLot Number
          577815

          References

          Reference overview
          del C. Alonso, A., et al. 2001. J. Biol. Chem. 276, 37967.
          Bennecib, M., et al. 2001. FEBS Lett. 490, 15.
          Sawamura, N., et al. 2001. J. Biol. Chem. 276, 10314.
          Tomidokoro, Y., et al. 2001. Neurosci. Lett. 299, 169.
          Bennecib, M., et al. 2000. FEBS Lett. 485, 87.
          Hashiguchi, M., et al. 2000. J. Biol. Chem. 275, 25247.
          Jenkins, S.M., et al. 2000. Biochem. J. 345, 263.
          Maas, T., et al., 2000. J. Biol. Chem. 275, 15733.
          Takahashi, M., et al. 2000. Brain. Res. 857, 193.
          Tesseur, I., et al. 2000. Am. J. Pathol. 156, 951.
          Schneider, A., et al. 1999. Biochemistry 38, 3549.
          Sengupta, A., et al. 1998. Arch. Biochem. Biophys. 357, 299.
          Wang, J.G., et al. 1998. FEBS Lett. 436, 28.
          Kopke, E., et al. 1993. J. Biol. Chem. 268, 24374.
          Arigada, P.A., et al. 1992. Neurology 42, 631.
          Drubin, D.G. and Kirschner, M.W. 1986. J. Cell. Biol. 103, 2739.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision16-August-2007 RFH
          ApplicationImmunoblotting (1:1000)
          Application Data
          Detection of human tau, phosphorylated at Ser396, by immunoblotting. Samples: Whole cell lysate from control cells with exogenously added human recombinant tau left untreated (lane 1) or treated with GSK-3β (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Tau (pSer396) Rabbit pAb (Cat. No. 577815) (1:1000) pre-incubated without peptide (lanes 1, 2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphoserine containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
          DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~45-68 kDa Tau protein phosphorylated at Ser396.
          BackgroundTau is the microtubule-associated protein predominantly found in neurons. It promotes assembly of tubulin into microtubules. Tau is also a phosphoprotein and its biological activity is regulated by the degree of phosphorylation. In the brain of Alzheimer's patients, tau is hyperphosphorylated and exists as both a cytosolic protein and as polymerized paired helical filaments (PHF). The PHF form the building blocks for neurofibrillary tangles that are the hallmark of AD brains. Hyperphosphorylated tau in AD inhibits the assembly of microtubules and causes disassembly of preassembled microtubules. Ultimately, hyperphosphorylated tau leads to the degeneration of the neurons and forms the pathogenic mechanism for Alzheimer's disease. In addition to AD brains, neurofibrillary tangles have also been observed in brains of patients with Niemann-Pick type C (NPC) disease. NPC is associated with the accumulation of cholesterol in several tissues and with progressive neurodegeneration. It has recently been shown that brains of NPC patients also accumulate neurofibrillary tangles without amyloid deposits. As in AD brains, the PHF of NPC brains are the result of hyperphosphorylation of tau. In tau, serine 396 is one of the four most prominent phosphorylation sites used by GSK-3.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁹⁶ phosphorylation site of human Tau
          IsotypeIgG
          Specieshuman
          Positive controlRecombinant human tau treated with GSK-3β
          FormLiquid
          FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.2-7.4.
          Preservative≤0.1% sodium azide
          CommentsWhen used at a 1:1000 dilution, there is sufficient antibody for 10 immunoblots at 10 ml/blot. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          Referencesdel C. Alonso, A., et al. 2001. J. Biol. Chem. 276, 37967.
          Bennecib, M., et al. 2001. FEBS Lett. 490, 15.
          Sawamura, N., et al. 2001. J. Biol. Chem. 276, 10314.
          Tomidokoro, Y., et al. 2001. Neurosci. Lett. 299, 169.
          Bennecib, M., et al. 2000. FEBS Lett. 485, 87.
          Hashiguchi, M., et al. 2000. J. Biol. Chem. 275, 25247.
          Jenkins, S.M., et al. 2000. Biochem. J. 345, 263.
          Maas, T., et al., 2000. J. Biol. Chem. 275, 15733.
          Takahashi, M., et al. 2000. Brain. Res. 857, 193.
          Tesseur, I., et al. 2000. Am. J. Pathol. 156, 951.
          Schneider, A., et al. 1999. Biochemistry 38, 3549.
          Sengupta, A., et al. 1998. Arch. Biochem. Biophys. 357, 299.
          Wang, J.G., et al. 1998. FEBS Lett. 436, 28.
          Kopke, E., et al. 1993. J. Biol. Chem. 268, 24374.
          Arigada, P.A., et al. 1992. Neurology 42, 631.
          Drubin, D.G. and Kirschner, M.W. 1986. J. Cell. Biol. 103, 2739.

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