ST1069 PhosphoDetect™ Anti-Paxillin (pSer¹⁷⁸) Rabbit pAb

ST1069
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      ST1069-50UG
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          Plastic ampoule 50 μg
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          Description
          OverviewRecognizes the ~68 kDa paxillin protein phosphorylated at Ser178.
          Catalogue NumberST1069
          Brand Family Calbiochem®
          Application Data
          Detection of mouse paxilin phosphorylated at Ser178, by immunoblotting. Samples: Whole cell lysate (~2.4 x 105 cells/lane) from NH3T3 cells serum-starved for 16 h and grown in suspension (lane 1) NIH3T3 cells plated on fibronectin (50 µg/ml for 1 h, lane 2), and NIH3T3 cells plated on fibronectin and treated with lamda phosphotase (lane 3). Primary antibody: Phospho-Dtect™ Anti-Paxilin (pSer178) Rabbit pAb (Cat. No. ST1069) (1:1000). Detection: chemiluminescence.
          References
          ReferencesBellis, S.L., et al. 1997. Biochem. J. 325, 375.
          Schaller, M.D. 2001. Oncogene 20, 6459.
          Huang, C., et al. 2003. Nature 424, 219.
          Product Information
          FormLiquid
          FormulationIn Tris-citrate/phosphate, pH 7.0-8.0.
          Positive controlNIH3T3 cells plated on fibronectin
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Application NotesImmunoblotting (1:1000-1:5000)
          Application CommentsDoes not recognize unphosphorylated paxillin. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁷⁸ phosphorylation site of human paxillin
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Mouse
          Antibody TypePolyclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Highly Toxic
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial use aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cylces.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          PhosphoDetect™ Anti-Paxillin (pSer¹⁷⁸) Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          PhosphoDetect™ Anti-Paxillin (pSer¹⁷⁸) Rabbit pAb Certificates of Analysis

          TitleLot Number
          ST1069

          References

          Reference overview
          Bellis, S.L., et al. 1997. Biochem. J. 325, 375.
          Schaller, M.D. 2001. Oncogene 20, 6459.
          Huang, C., et al. 2003. Nature 424, 219.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision18-October-2007 RFH
          ApplicationImmunoblotting (1:1000-1:5000)
          Application Data
          Detection of mouse paxilin phosphorylated at Ser178, by immunoblotting. Samples: Whole cell lysate (~2.4 x 105 cells/lane) from NH3T3 cells serum-starved for 16 h and grown in suspension (lane 1) NIH3T3 cells plated on fibronectin (50 µg/ml for 1 h, lane 2), and NIH3T3 cells plated on fibronectin and treated with lamda phosphotase (lane 3). Primary antibody: Phospho-Dtect™ Anti-Paxilin (pSer178) Rabbit pAb (Cat. No. ST1069) (1:1000). Detection: chemiluminescence.
          DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~68 kDa paxillin protein phosphorylated at Ser178.
          BackgroundPaxillin is a focal adhesion adaptor protein associated with th establishment of focal adhesions, cell signaling via integrins, and cell motility. The N-terminus of paxillin contains a proline-rich region for SH3 and SH2 domain binding. The N-terminus also contains five copies of the LD motif for association of actin binding proteins. The C-terminus contains LIM domains and the focal adhesion targeting sequence. Paxillin is believed to function as an adaptor protein that facilitates assembly of multi-protein complexes and coordinates signaling via integrins. Tyrosine and serine phosphorylation of paxillin is associated with the establishment of cytoskeletal membrane attachments. Phosphorylation by JNK at Ser178 is necessary for normal cell motility.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁷⁸ phosphorylation site of human paxillin
          IsotypeIgG
          Specieshuman, mouse
          Positive controlNIH3T3 cells plated on fibronectin
          FormLiquid
          FormulationIn Tris-citrate/phosphate, pH 7.0-8.0.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsDoes not recognize unphosphorylated paxillin. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial use aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cylces.
          Toxicity Highly Toxic
          ReferencesBellis, S.L., et al. 1997. Biochem. J. 325, 375.
          Schaller, M.D. 2001. Oncogene 20, 6459.
          Huang, C., et al. 2003. Nature 424, 219.