442685 PhosphoDetect™ Anti-MAP Kinase ERK1/ERK2 (pThr²⁰²/Tyr²⁰⁴) Rabbit pAb

442685
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      442685-100UL
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          Plastic ampoule 100 ul
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          Description
          OverviewRecognizes the ~42-44 kDa ERK1 and ERK2 proteins phosphorylated at Thr202 and Tyr204.
          Catalogue Number442685
          Brand Family Calbiochem®
          Application Data
          Detection of human MAP Kinase ERK1/ERK2, phosphorylated on Thr202/Tyr204, by immunoblotting. Samples: Whole cell lysate from human T47D cells untreated (lane 1) or treated with lambda-phosphatase (lane 2). Primary antibody: PhosphoDetect™ Anti-MAP Kinase ERK1/ERK2 (pThr202/Tyr204) Rabbit pAb (Cat. No. 442685) (1:1000). Detection: chemiluminescence.
          References
          Product Information
          FormLiquid
          FormulationIn 150 mM NaCl, 10 mM HEPES, 100 mg/ml BSA, 50% glycerol, pH 7.5.
          PreservativeNone
          Applications
          Key Applications Dot Blot
          Immunoblotting (Western Blotting)
          Application NotesDot Blot (1:1000)
          Immunoblotting (1:1000)
          Application CommentsDoes not react with the unphosphorylated proteins. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogena synthetic phosphopeptide surrounding the Thr²⁰²/Tyr²⁰⁴ phosphorylation sites of human ERK1/ERK2
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          442685

          Brochure

          Title
          Antibody Sourcebook!" Second Edition
          Biologics 32.3 PDF ( 927 KB )
          MAPK Pathway Poster ( 750 KB )
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision07-August-2007 RFH
          ApplicationDot Blot (1:1000)
          Immunoblotting (1:1000)
          Application Data
          Detection of human MAP Kinase ERK1/ERK2, phosphorylated on Thr202/Tyr204, by immunoblotting. Samples: Whole cell lysate from human T47D cells untreated (lane 1) or treated with lambda-phosphatase (lane 2). Primary antibody: PhosphoDetect™ Anti-MAP Kinase ERK1/ERK2 (pThr202/Tyr204) Rabbit pAb (Cat. No. 442685) (1:1000). Detection: chemiluminescence.
          DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~42-44 kDa ERK1 and ERK2 proteins phosphorylated at Thr202 and Tyr204.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic phosphopeptide surrounding the Thr²⁰²/Tyr²⁰⁴ phosphorylation sites of human ERK1/ERK2
          IsotypeIgG
          Specieshuman, rat
          FormLiquid
          FormulationIn 150 mM NaCl, 10 mM HEPES, 100 mg/ml BSA, 50% glycerol, pH 7.5.
          PreservativeNone
          CommentsDoes not react with the unphosphorylated proteins. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling

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          Categories

          Life Science Research > Antibodies and Assays > Primary Antibodies