324865 | PhosphoDetect™ Anti-EGFR (pTyr⁸⁴⁵) Rabbit pAb

324865
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      324865-10T
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          Plastic ampoule 10 t
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          Description
          OverviewRecognizes the ~170 kDa EGFR protein phosphorylated at Tyr845 in EGF-treated A431 cells. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
          Catalogue Number324865
          Brand Family Calbiochem®
          SynonymsAnti-Epidermal Growth Factor Receptor (pTyr⁸⁴⁵)
          Application Data
          Detection of human epidermal growth factor receptor, phosphorylated on Tyr845, by immunoblotting. Samples: Whole cell lysate from A431 cells left unstimulated (lane 1) or stimulated with EGF (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Epidermal Growth Factor Receptor (pTyr845) Rabbit pAb (Cat. No. 324865) (1:1000) pre-incubated without peptide (lanes 1, 2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
          References
          ReferencesSieg, D.J. et al. 2000. Nat. Cell. Biol. 2, 249.
          Barbier, A.J. et al. 1999. J. Biol. Chem. 274, 14067.
          Biscardi, J.S., et al. 1999. J. Biol. Chem. 274, 8335.
          Hashimoto, A. et al. 1999. J. Biol. Chem. 274, 20139
          Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys. 363, 227.
          Product Information
          FormLiquid
          FormulationIn Dulbecco's PBS (w/o Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.4.
          Positive controlA431 cells stimulated with EGF
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Application NotesImmunoblotting (1:1000)
          Application CommentsThe immunogen sequence is 100% conserved in mouse and rat and 93% conserved in chicken EGFR, but cross-reactivity has not been tested. When diluted to 1:1000 there is sufficient antibody to perform 10 miniblots (10 T) at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁸⁴⁵ phosphorylation site of human EGFR
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          324865

          References

          Reference overview
          Sieg, D.J. et al. 2000. Nat. Cell. Biol. 2, 249.
          Barbier, A.J. et al. 1999. J. Biol. Chem. 274, 14067.
          Biscardi, J.S., et al. 1999. J. Biol. Chem. 274, 8335.
          Hashimoto, A. et al. 1999. J. Biol. Chem. 274, 20139
          Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys. 363, 227.

          Brochure

          Title
          Antibody Sourcebook!" Second Edition
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision01-August-2007 RFH
          SynonymsAnti-Epidermal Growth Factor Receptor (pTyr⁸⁴⁵)
          ApplicationImmunoblotting (1:1000)
          Application Data
          Detection of human epidermal growth factor receptor, phosphorylated on Tyr845, by immunoblotting. Samples: Whole cell lysate from A431 cells left unstimulated (lane 1) or stimulated with EGF (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Epidermal Growth Factor Receptor (pTyr845) Rabbit pAb (Cat. No. 324865) (1:1000) pre-incubated without peptide (lanes 1, 2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
          DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site and a generic tyrosine phosphorylated peptide, followed by immunoaffinity chromatography. Recognizes the ~185 KDa EGFR protein phosphorylated on Tyr845.
          BackgroundEpidermal Growth Factor (EGF) is responsible for a variety of biological effects including mitogenesis and differentiation. The EGF receptor (EGFR) has three domains: the extracellular, transmembrane, and cytoplasmic domain. Upon the binding of EGF to the extracellular domain, dimerization of the receptor occurs and the cytoplasmic domain displays tyrosine kinase activity. This leads to the autophosphorylation of the ~170 kDa glycoprotein on various residues within the cytoplasmic domain as well as the phosphorylation of intracellular substrates. Tyr845 is an autophosphorylation site within EGFR. Phosphorylation of tyrosine 845 is mediated by non-receptor tyrosine kinase c-Src.
          HostRabbit
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁸⁴⁵ phosphorylation site of human EGFR
          IsotypeIgG
          Specieshuman
          Positive controlA431 cells stimulated with EGF
          FormLiquid
          FormulationIn Dulbecco's PBS (w/o Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.4.
          Preservative≤0.1% sodium azide
          CommentsThe immunogen sequence is 100% conserved in mouse and rat and 93% conserved in chicken EGFR, but cross-reactivity has not been tested. When diluted to 1:1000 there is sufficient antibody to perform 10 miniblots (10 T) at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesSieg, D.J. et al. 2000. Nat. Cell. Biol. 2, 249.
          Barbier, A.J. et al. 1999. J. Biol. Chem. 274, 14067.
          Biscardi, J.S., et al. 1999. J. Biol. Chem. 274, 8335.
          Hashimoto, A. et al. 1999. J. Biol. Chem. 274, 20139
          Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys. 363, 227.

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