324867 PhosphoDetect™ Anti-EGFR (pTyr¹⁰⁶⁸) Rabbit pAb

324867
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      Ch, H, M, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      324867-10T
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          Plastic ampoule 10 t
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          Description
          OverviewRecognizes the ~185 kDa EGFR protein phosphorylated at Tyr1068. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
          Catalogue Number324867
          Brand Family Calbiochem®
          SynonymsAnti-Epidermal Growth Factor Receptor (pTyr¹⁰⁶⁸)
          Application Data
          Detection of human EGFR, phosphorylated at Tyr1068, by immunoblotting. Samples: Whole cell lysate from A431 cells unstimulated (lane 1), or stimulated for EGF (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Epidermal Growth Factor Receptor (pTyr1068) Rabbit pAb (Cat. No. 324867) (1:1000) pre-incubated without peptide (lanes 1 and 5), with phosphopeptide immunogen (lane 2), a generic phosphotyrosine-containing peptide (lane 3), or non-phosphopeptide corresponding to the immunogen (lane 4). Detection: chemiluminescence.
          References
          ReferencesJo, M., et al. 2000. J. Biol. Chem. 275, 8806.
          Maudsley, S., et al. 2000. J. Biol. Chem. 275, 9572.
          Sieg, D.J. et al. 2000. Nat. Cell. Biol. 2, 249.
          Barbier, A.J. et al. 1999. J. Biol. Chem. 274, 14067.
          Hashimoto, A. et al. 1999. J. Biol. Chem. 274, 20139
          Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys. 363, 227.
          Batzer, A.G. et al. 1994. Mol. Cell. Biol. 14, 5192.
          Product Information
          FormLiquid
          FormulationIn Dulbecco's PBS, 1 mg/ml BSA, 50% glycerol, pH 7.4.
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Application NotesImmunoblotting (1:1000)
          Application CommentsWhen diluted 1:1000 there is sufficient antibody to perform 10 miniblots (10 T) at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹⁰⁶⁸ phosphorylation site of human EGFR
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Chicken
          • Human
          • Mouse
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          324867

          References

          Reference overview
          Jo, M., et al. 2000. J. Biol. Chem. 275, 8806.
          Maudsley, S., et al. 2000. J. Biol. Chem. 275, 9572.
          Sieg, D.J. et al. 2000. Nat. Cell. Biol. 2, 249.
          Barbier, A.J. et al. 1999. J. Biol. Chem. 274, 14067.
          Hashimoto, A. et al. 1999. J. Biol. Chem. 274, 20139
          Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys. 363, 227.
          Batzer, A.G. et al. 1994. Mol. Cell. Biol. 14, 5192.

          Brochure

          Title
          Antibody Sourcebook!" Second Edition
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision03-March-2008 JSW
          SynonymsAnti-Epidermal Growth Factor Receptor (pTyr¹⁰⁶⁸)
          ApplicationImmunoblotting (1:1000)
          Application Data
          Detection of human EGFR, phosphorylated at Tyr1068, by immunoblotting. Samples: Whole cell lysate from A431 cells unstimulated (lane 1), or stimulated for EGF (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Epidermal Growth Factor Receptor (pTyr1068) Rabbit pAb (Cat. No. 324867) (1:1000) pre-incubated without peptide (lanes 1 and 5), with phosphopeptide immunogen (lane 2), a generic phosphotyrosine-containing peptide (lane 3), or non-phosphopeptide corresponding to the immunogen (lane 4). Detection: chemiluminescence.
          DescriptionRabbit polyclonal antibody adsorbed against non-phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~170 kDa EGFR protein phosphorylated at Tyr1068.
          BackgroundEpidermal Growth Factor (EGF) is responsible for a variety of biological effects including mitogenesis and differentiation. The EGF receptor (EGFR) has three domains: the extracellular, transmembrane, and cytoplasmic domains. Upon binding of EGF to the extracellular domain, dimerization of the receptor occurs and the cytoplasmic domains display tyrosine kinnase activity. This leads to autophosphorylation of the ~170 kDa glycoprotein on various residues within the cytoplasmic domain as well as phosphorylation of intracellular substrates. Tyr1068 is an autophosphorylation site in EGFR. The SH2 domain of Grb2 binds to Tyr1068 of EGFR in the multimeric complex EGFR-Grb2-mSos1 that is formed upon stimulation of EGFR with EGF.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹⁰⁶⁸ phosphorylation site of human EGFR
          IsotypeIgG
          Specieschicken, human, mouse, rat
          FormLiquid
          FormulationIn Dulbecco's PBS, 1 mg/ml BSA, 50% glycerol, pH 7.4.
          Preservative≤0.1% sodium azide
          CommentsWhen diluted 1:1000 there is sufficient antibody to perform 10 miniblots (10 T) at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesJo, M., et al. 2000. J. Biol. Chem. 275, 8806.
          Maudsley, S., et al. 2000. J. Biol. Chem. 275, 9572.
          Sieg, D.J. et al. 2000. Nat. Cell. Biol. 2, 249.
          Barbier, A.J. et al. 1999. J. Biol. Chem. 274, 14067.
          Hashimoto, A. et al. 1999. J. Biol. Chem. 274, 20139
          Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys. 363, 227.
          Batzer, A.G. et al. 1994. Mol. Cell. Biol. 14, 5192.