DR1025 PhosphoDetect™ Anti-Chk1 (pSer³¹⁷) Rabbit pAb

DR1025
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, Mk, M, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      DR1025-50UL
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          Plastic ampoule 50 ul
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          Description
          OverviewRecognizes the ~56 kDa Chk1 protein phosphorylated at Ser317 in UV-treated COS cells.
          Catalogue NumberDR1025
          Brand Family Calbiochem®
          Application Data
          Detection of human and monkey Chk1, phosphorylated at Ser317, by immunoblotting. Samples: Whole cell lysate from HeLa cells treated with hydroxyurea (Cat. No. 400046) or COS cells treated with hydroxyurea or UV for the indicated times. Primary antibody: PhosphoDetect™ Anti-Chk1 (pSer317) Rabbit pAb (Cat. No. DR1025) (1:1000). Detection: chemiluminescence.
          References
          ReferencesZhao, H. and Piwnica-Worms, H. 2001. Mol. Cell. Biol. 21, 4129.
          Shieh, S.Y., et al. 2000. Genes Dev. 14, 289.
          Martinho, R.G., et al. 1998. EMBO J. 17, 7239.
          Zeng, Y., et al. 1998. Nature 395, 507.
          Product Information
          FormLiquid
          FormulationIn 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
          Positive controlLysates from COS cells treated with UV
          PreservativeNone
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Immunocytochemistry
          Paraffin Sections
          Application NotesImmunoblotting (1:1000)
          Immunocytochemistry (1:500, fluorescence)
          Paraffin Sections (1:50)
          Application CommentsDoes not recognize unphosphorylated Chk1. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser³¹⁷ phosphorylation site of human Chk1
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Monkey
          • Mouse
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsDo not aliquot.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          PhosphoDetect™ Anti-Chk1 (pSer³¹⁷) Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          PhosphoDetect™ Anti-Chk1 (pSer³¹⁷) Rabbit pAb Certificates of Analysis

          TitleLot Number
          DR1025

          References

          Reference overview
          Zhao, H. and Piwnica-Worms, H. 2001. Mol. Cell. Biol. 21, 4129.
          Shieh, S.Y., et al. 2000. Genes Dev. 14, 289.
          Martinho, R.G., et al. 1998. EMBO J. 17, 7239.
          Zeng, Y., et al. 1998. Nature 395, 507.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision23-August-2007 RFH
          ApplicationImmunoblotting (1:1000)
          Immunocytochemistry (1:500, fluorescence)
          Paraffin Sections (1:50)
          Application Data
          Detection of human and monkey Chk1, phosphorylated at Ser317, by immunoblotting. Samples: Whole cell lysate from HeLa cells treated with hydroxyurea (Cat. No. 400046) or COS cells treated with hydroxyurea or UV for the indicated times. Primary antibody: PhosphoDetect™ Anti-Chk1 (pSer317) Rabbit pAb (Cat. No. DR1025) (1:1000). Detection: chemiluminescence.
          DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~56 kDa Chk1 protein when phosphorylated at Ser317.
          BackgroundChk1 is a downstream protein kinase of ATM/ATR that plays an improtant role in DNA damage checkpoint, development, and tumor suppression. Chk1 is activated in response to various forms of genotoxic stress and replication blocks. Chk1 is activated by phosphorylation on Ser317 and Ser345. Chk1 can inactivate cdc25c and phosphorylate p53.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser³¹⁷ phosphorylation site of human Chk1
          IsotypeIgG
          Specieshuman, monkey, mouse, rat
          Positive controlLysates from COS cells treated with UV
          FormLiquid
          FormulationIn 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
          PreservativeNone
          CommentsDoes not recognize unphosphorylated Chk1. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsDo not aliquot.
          Toxicity Standard Handling
          ReferencesZhao, H. and Piwnica-Worms, H. 2001. Mol. Cell. Biol. 21, 4129.
          Shieh, S.Y., et al. 2000. Genes Dev. 14, 289.
          Martinho, R.G., et al. 1998. EMBO J. 17, 7239.
          Zeng, Y., et al. 1998. Nature 395, 507.