SCR015 Pancreatic Cell Culture Supplement

SCR015
50 mL  20X concentrate
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      CULT
      Description
      Catalogue NumberSCR015
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionPancreatic Cell Culture Supplement
      OverviewPancreatic Cell Culture Supplement is intended for the preparation of complete cell culture medium in conjunction with Pancreatic Cell Culture Medium (SCR016). This complete medium is optimized for the culture of LT2 Immortalized Pancreatic Mesenchymal Cells (SCR013) and VIT1 Primary Pancreatic Mesenchymal Cells (SCR014).
      References
      Product Information
      Applications
      ApplicationThe Pancreatic cell culture Supplement is intended for the preparation of complete cell culture medium in conjunction with Pancreatic cell culture Medium (SCR016).
      Key Applications
      • Cell Culture
      Application NotesThe Pancreatic Cell Culture Supplement is provided as a 20X concentrate. The following table provides useful quantities of Pancreatic Cell Culture Supplement and Pancreatic Cell Culture Medium for cell culture.



      Total Volume(mL) / Pancreatic Cell Culture Supplement (mL) / Pancreatic Cell Culture Medium (mL)

      5 / 0.25 / 4.75

      10 / 0.50 / 9.50

      15 / 0.75 / 14.25

      20 / 1.00 / 19.00

      50 / 2.50 / 47.50



      For optimum viability of the LT2 Immortalized Pancreatic Mesenchymal Cells (SCR013) and VIT1 Primary Pancreatic Mesenchymal Cells (SCR014), it is recommended that the Pancreatic Cell Culture Medium be maintained within the tissue incubator, equilibrated to 37°C and 5% CO2 while the VitroPlus II concentrate is stored at 4°C. Use a container such as a vented tissue culture flask to store basal medium to ensure gas exchange with the medium since it is buffered with sodium bicarbonate. Complete medium is then prepared just prior to use by 20-fold dilution, according to the above table.
      Biological Information
      Media FormLiquid
      Stem Cell Type
      • Pancreatic Stem Cells
      • Mesenchymal Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe Pancreatic Cell Culture Supplement concentrate is provided frozen. Prior to use, thaw the entire bottle at room temperature or at refrigerator temperature overnight. Aliquot and store according to usage needs. The concentrate is stable until expiration (2 years from date of manufacture) at -20°C or for one month at 4°C. Four 12.5 mL aliquots are useful for most applications, with three aliquots maintained at -20°C (12.5 mL of concentrate is sufficient to prepare 250 mL of complete medium).
      Packaging Information
      Material Size50 mL
      Material Package20X concentrate
      Transport Information
      Supplemental Information
      Specifications