53702 PRONASE® Protease, Streptomyces griseus

53702
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      53702-10KU
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          53702-250KU
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              53702-25KU
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                  53702-500KU
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                      Plastic ampoule 500 ku
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                      53702-50KU
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                          53702-KU
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                              Glass bottle
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                              Description
                              OverviewA non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at neutral pH, but stable over wide range of pH and temperature. Note: 1KU = 1000 units.
                              Note: 1 KU = 1000 units.
                              Catalogue Number53702
                              Brand Family Calbiochem®
                              References
                              ReferencesNarahashi, Y., et al. 1968. J. Biochem. 64, 427.
                              Product Information
                              CAS number9036-06-0
                              Activity≥45,000 proteolytic units/g dry weight
                              Unit of DefinitionOne unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.
                              FormLyophilized
                              FormulationLyophilized from a solution containing calcium acetate as a stabilizer.
                              Hygroscopic Hygroscopic
                              Applications
                              Biological Information
                              Physicochemical Information
                              Dimensions
                              Materials Information
                              Toxicological Information
                              Safety Information according to GHS
                              RTECSUK9595000
                              Safety Information
                              R PhraseR: 20-36/37/38-42/43

                              Harmful by inhalation.
                              Irritating to eyes, respiratory system and skin.
                              May cause sensitization by inhalation and skin contact.
                              S PhraseS: 22-26-36

                              Do not breathe dust.
                              In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
                              Wear suitable protective clothing.
                              Product Usage Statements
                              Storage and Shipping Information
                              Ship Code Ambient Temperature Only
                              Toxicity Harmful
                              Storage +2°C to +8°C
                              Hygroscopic Hygroscopic
                              Do not freeze Ok to freeze
                              Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for 24 h at 4°C.
                              Packaging Information
                              Transport Information
                              Supplemental Information
                              Specifications

                              Documentation

                              PRONASE® Protease, Streptomyces griseus SDS

                              Title

                              Safety Data Sheet (SDS) 

                              PRONASE® Protease, Streptomyces griseus Certificates of Analysis

                              TitleLot Number
                              53702

                              References

                              Reference overview
                              Narahashi, Y., et al. 1968. J. Biochem. 64, 427.
                              Data Sheet

                              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

                              Revision17-September-2007 RFH
                              DescriptionA non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at neutral pH, but it is stable over a wide pH and temperature range. Has an optimal pH of 7.5. Note: 1 KU = 1000 units.
                              FormLyophilized
                              FormulationLyophilized from a solution containing calcium acetate as a stabilizer.
                              CAS number9036-06-0
                              RTECSUK9595000
                              Activity≥45,000 proteolytic units/g dry weight
                              Unit definitionOne unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.
                              SolubilityH₂O (10 mg/ml)
                              Storage +2°C to +8°C
                              Hygroscopic
                              Do Not Freeze Ok to freeze
                              Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for 24 h at 4°C.
                              Toxicity Harmful
                              ReferencesNarahashi, Y., et al. 1968. J. Biochem. 64, 427.