476480 PKC 20-28, Cell-Permeable, Myristoylated - Calbiochem

Pseudosubstrate sequence from protein kinase Cα and β ( PKCα and PKCβ).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Myr-N-FARKGALRQ-NH₂, Myristoylated Protein Kinase C Inhibitor 20-28, Cell-Permeable, Protein Kinase C 20-28, Cell-Permeable, Myristoylated

Primary Target: TPA activation of MARCKS phosphorylation in fibroblast primary cultures
476480
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical Formula
      C₆₀H₁₀₆N₁₈O₁₁

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      476480-500UG
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          		 Plastic ampoule 500 μg
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          Description
          OverviewPseudosubstrate sequence from protein kinase Cα and β ( PKCα and PKCβ). N-Terminus is myristoylated to allow membrane permeability. Highly specific, reversible, and substrate competitive inhibitor of TPA activation of MARCKS phosphorylation in fibroblast primary cultures (IC50 = 8 µM); exhibits 98% inhibition at 100 µM.
          Catalogue Number476480
          Brand Family Calbiochem®
          SynonymsMyr-N-FARKGALRQ-NH₂, Myristoylated Protein Kinase C Inhibitor 20-28, Cell-Permeable, Protein Kinase C 20-28, Cell-Permeable, Myristoylated
          References
          ReferencesEichholtz, T., et al. 1993. J. Biol. Chem. 268, 1982.
          Ward, N.E. and O’Brian, C.A. 1993. Biochemistry 32, 11903.
          Product Information
          ATP CompetitiveN
          FormLyophilized solid
          FormulationSupplied as a trifluoroacetate salt.
          Hill FormulaC₆₀H₁₀₆N₁₈O₁₁
          Chemical formulaC₆₀H₁₀₆N₁₈O₁₁
          Hygroscopic Hygroscopic
          ReversibleY
          Sold on the basis of peptide contentY
          Applications
          Biological Information
          Primary TargetTPA activation of MARCKS phosphorylation in fibroblast primary cultures
          Primary Target IC<sub>50</sub>8 µM against TPA activation of MARCKS phosphorylation in fibroblast primary cultures
          Purity≥95% by HPLC
          Physicochemical Information
          Cell permeableY
          Peptide ContentY
          Peptide SequenceN-Myr-Phe-Ala-Arg-Lys-Gly-Ala-Leu-Arg-Gln-NH₂
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Hygroscopic Hygroscopic
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          476480

          References

          Reference overview
          Eichholtz, T., et al. 1993. J. Biol. Chem. 268, 1982.
          Ward, N.E. and O’Brian, C.A. 1993. Biochemistry 32, 11903.

          Brochure

          Title
          PKC Pathway Poster PDF ( 676 KB )

          Citations

          Title
        • Ying Zhang, Mingjuan Liao and Maria L. Dufau. (2006) Phosphatidylinositol 3-kinase/protein kinase Cγ-induced phosphorylation of Sp1 and p107 repressor release have a critical role in histone deacetylase inhibitor-mediated depression of transcription of the luteinizing hormone receptor gene. Molecular and Cellular Biology 26, 6748-6761.
          		•
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision11-January-2010 RFH
          SynonymsMyr-N-FARKGALRQ-NH₂, Myristoylated Protein Kinase C Inhibitor 20-28, Cell-Permeable, Protein Kinase C 20-28, Cell-Permeable, Myristoylated
          DescriptionPseudosubstrate sequence from protein kinase Ca (PKCα) and PKCβ. N-terminal myristoylated to allow membrane permeability. Highly specific, reversible, and substrate competitive inhibitor of TPA activation of MARCKS phosphorylation in fibroblast primary cultures (IC50 = 8 µM). Exhibits 98% inhibition at 100 µM.
          FormLyophilized solid
          FormulationSupplied as a trifluoroacetate salt.
          Chemical formulaC₆₀H₁₀₆N₁₈O₁₁
          Peptide SequenceN-Myr-Phe-Ala-Arg-Lys-Gly-Ala-Leu-Arg-Gln-NH₂
          Purity≥95% by HPLC
          SolubilityTris-HCl, pH 7.5 (25 mg/ml) or H₂O (10 mg/ml)
          Storage -20°C
          Hygroscopic
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.
          Toxicity Standard Handling
          ReferencesEichholtz, T., et al. 1993. J. Biol. Chem. 268, 1982.
          Ward, N.E. and O’Brian, C.A. 1993. Biochemistry 32, 11903.
          Citation
        • Ying Zhang, Mingjuan Liao and Maria L. Dufau. (2006) Phosphatidylinositol 3-kinase/protein kinase Cγ-induced phosphorylation of Sp1 and p107 repressor release have a critical role in histone deacetylase inhibitor-mediated depression of transcription of the luteinizing hormone receptor gene. Molecular and Cellular Biology 26, 6748-6761.
          		•