PCR Cleanup Filter Plates

Remove primers and unincorporated dNTPs in one step

Overview

Specifications

Ordering Information

Filter Plates Clear Sorting & Filtering
Catalog Numbericon Descriptionicon Pack Sizeicon
LSKMPCR10MultiScreen PCRµ96 Filter Plate 10 Show Pricing & Availability
LSKMPCR50MultiScreen-PCRµ96 Filter Plate 50 Show Pricing & Availability
MSNU03010MultiScreen-PCR96 Filter Plate 10 Show Pricing & Availability
MSNU03050MultiScreen-PCR96 Filter Plate 50 Show Pricing & Availability
S384PCR10MultiScreen PCR384 Filter Plate 10 Show Pricing & Availability
S384PCR50MultiScreen PCR384 Filter Plate 50 Show Pricing & Availability

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Required Equiment Clear Sorting & Filtering
Catalog Numbericon Descriptionicon Pack Sizeicon
MSVMHTS00MultiScreenHTS Vacuum Manifold 1 Show Pricing & Availability
WP6110060Chemical Duty Pump, 100 V/50–60 Hz 1 Show Pricing & Availability
WP6111560Chemical Duty Pump, 115 V/60 Hz 1 Show Pricing & Availability
WP6122050Chemical Duty Pump, 220 V/50 Hz 1 Show Pricing & Availability

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    Documentation

    Data Sheet

    Title
    DNA Template Prep and Sequencing Reaction Cleanup
    MultiScreen 384-PCR Filter Plate: Automation-friendly 384-well plate for high-throughput purification of PCR* products

    Posters

    Title
    Poster: Montage PCRµ96 and SEQ96: A Total Solution for Sequencing PCR-derived Templates
    Poster: Purification Automation of Small Volume PCR Purification

    References

    Reference overviewApplication
    PCR Amplification on a Microarray of Gel-Immobilized Oligonucleotides: Detection of Bacterial Toxin- and Drug-Resistant Genes and Their Mutations
    Strizhkov, B.N. Drobyshev, A.L. Mikhailovich, V.M. Mirzabekov, A.D.
    BioTechniques 29:844-857  2000

    A Concise Guide to cDNA Microarray Analysis
    P. Hegde, R. Qi, K. Abernathy, C. Gay, S. Dharap, R. Gaspard, J.E. Hughes, E. Snesrud, N. Lee, and J. Quackenbush
    BioTechniques Vol. 29, No. 3: pp 548-562 (Sep 2000)  2000

    PCR
    A specific and sensitive PCR assay suitable for large-scale detection of toxigenic Pasteurella multocida in nasal and tonsillar swabs specimens of pigs
    Kamp, E.M. etal., J.Vet Diagn Invest 8:, 304-309
    J.Vet Diagn Invest 8:, 304-309  1996

    Nucleic Acid Purification and Concentration
    Use of MultiScreen Plates for the Preparation of Bacterial DNA Suitable for PCR
    Reek, F.H., Smits, M.A., Kamp, E. M., Smith, H.E.
    BioTechniques. 19: 2, 282 – 285  1995

    Nucleic Acid Purification and Concentration

    FAQ

    QuestionAnswer
    Can I use Montage PCR plates for purification of RNAi?Many researchers have used the PCR plates for RNAi purification. Millipore has not validated these protocols in-house, but information can be found at the following websites:

    For general information:
    http://www.flyrnai.org

    For protocol:
    http://flyrnai.org/DRSC-PRS.html

    The Montage PCR 96 plate (#MANU03050) is used in this protocol (This protocol should also be adaptable to the 384 and µ96 PCR plates). The sample is diluted up to 100 ul and filtered as per the Millipore user guide instructions. For more detail on that step:

    • Purification of RNAi with MultiScreen PCR plates (from a 40 mL reaction)
    • After transcription, add 58 µl of water and 2 µl DNase to each well to bring the volume to 100 ul.
    • Transfer the samples to the MultiScreen PCR plate and place the plate on a vacuum manifold (#MAVM 096 0R), set vacuum at 5” Hg and vacuum for approximately 30 - 45 minutes or until all of the wells are dry.
    • After vacuuming, add 100 µl 10 mM Tris pH 7 to each well of MultiScreen plate.
    • Cover plate with foil seal and shake on plate shaker for 30 minutes. Do not shake too vigorously, as these plates cannot be spun in a centrifuge.
    • Add an additional 100 µl of 10 mM Tris pH 7 to each well and then transfer to a storage plate.
    Can the Montage PCR-96 Kit be used in a procedure employing centrifugal rather than vacuum filtration?Use of the Montage PCR-96 Kit in a centrifugal protocol is not recommended.
    Can the MultiScreen plates included with the Montage kit be used with a vacuum manifold from a vendor other than Millipore?Yes, but only if proper underdrain support grid is employed. Please contact your local Millipore technical service organization for specific information on the availability of the "underdrain supports" for non-Millipore vacuum manifolds.
    Is a wash step required following the initial filtration with the Montage PCR-96 Cleanup Kit?For many applications, a wash step is not necessary. If you need wash the purified PCR products, use the nuclease free water included in the PCR cleanup kit.
    Should I use water or Tris buffer to resuspend the final PCR products?Either solution may be used with the Montage PCR-96 Kit. A user may prefer one of these depending on the downstream application.
    What are the minimum and maximum loading capacities for the MultiScreen plates used with the Montage PCR-96 Kit?There is a 1 ug minimum capacity and no maximum limit.
    What is filtration time for the the Montage PCR-96 Kit?Filtration time is approximately 10 min, depending on volume of sample.
    Is the Montage PCR-96 kit capable of eliminating Triton or other detergents from the buffers used in PCR reactions?It is difficult to completely remove Triton. We recommend the use of a PCR buffer that is Triton-free. Alternatively, one can use MultiScreen FB plates with TN063 protocol.
    Can the MultiScreen-PCR plate be used in a centrifugal mode?The MultiScreen-PCR plates are designed for use in the vacuum mode and use in the centrifugal mode is not recommended.
    Can the MultiScreen-PCR plate be used with another manufacturers vacuum manifold?No, the plate needs the support grid of the Millipore vacuum manifold.

    User Guides

    Title
    Montage PCR96 Clean-Up Kit Laminated Card
    Montage PCR96 Clean-Up Kit User Guide
    MultiScreen 384 PCR Plate
    MultiScreen PCR µ96 Plate