S7250 OxyELISA™ Oxidized Protein Quantitation Kit

S7250
192 tests  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      Oxidized Protein M, H, R, Po, Eq, Rb, Gp, Ht, NhP, Ca ELISA Colorimetric
      Description
      Catalogue NumberS7250
      Trade Name
      • OxyELISA
      DescriptionOxyELISA™ Oxidized Protein Quantitation Kit
      OverviewThe OxyELISA™ Oxidized Protein Quantitation Kit provides a sensitive methodology for detection and quantitation of proteins modified by oxygen free radicals and other reactive species. Oxygen-derived free radicals have been implicated in numerous physiological functions such as apoptosis, cancer, aging, neurodegenerative diseases, chronic inflammatory diseases, pulmonary diseases, and cardiovascular diseases (for reviews, see ref. 1-4).

      Oxygen free radicals are generated by environmental factors such as ionizing radiation and chemical substances. They are also produced during normal cellular metabolism by mitochondrial electron transport, the cellular redox system, and by immune responses (1, 5-7). Although cells have developed various antioxidant defenses to protect against free radicals (7-9), free radicals that escape the defenses attack and modify subcellular components – nucleic acids, lipids and proteins (1, 10-12). In some cases, cells respond to the oxidative stimuli and allow the organism to adapt to the oxidative stress (13-15).

      Proteins are one of the major targets of oxygen free radicals and other reactive species. Oxidation of proteins modifies the side chains of methionine, histidine, and tyrosine and forms cysteine disulfide bonds (16-19). Metal catalyzed oxidation of proteins introduces carbonyl groups (aldehydes and ketones) at lysine, arginine, proline or threonine residues in a site-specific manner (1, 16, 20-22).

      The oxidative modification of proteins can modulate biochemical characteristics of proteins such as enzymatic activity (21-23), DNA binding activities of transcription factors (24-26), and the susceptibility to proteolytic degradation (12, 25-28). While a relationship between protein oxidation and aging has been suggested (29-31), little is known about the importance of oxidative modification of individual proteins in the pathophysiology of free radical mediated processes.

      In conclusion, the OxyELISA Oxidized Protein Quantitation Kit provides chemical and immunological reagents necessary to perform quantitative detection of carbonyl groups introduced into proteins by oxidative reactions with ozone or oxides of nitrogen or by metal catalyzed oxidation.

      For Research Use Only; Not for use in diagnostic procedures
      References
      Product Information
      Components
      • 2,4-Dinitrophenylhydrazine (DNPH) Solution
      • 1.1mL
        Oxidized Protein Standard (Lyophilized)
      • 1 vial
        Reduced Protein Standard (Lyophilized)
      • 1 vial
        5x Lysis Buffer/Protein Diluent
      • 20mL
        10x Blocking Buffer
      • 6mL
        10x Wash Buffer
      • 130mL
        Anti-DNP Mouse Monoclonal HRP Conjugated Antibody
      • 22μL
        3,3’,5,5’-Tetramethylbenzidine (TMB) Solution
      • 45mL
        Stop Solution
      • 12mL
        MILLEX® GP Syringe Driven Filter Unit (0.22μm)(Catalog No. SLGP033RS)
      • 3 Filters
        Adhesive Plate Sealing Films
      • 6 Films
        High Protein Binding Assay Plate
      • 2 Plates (96-well)
      Detection methodColorimetric
      Applications
      ApplicationThe OxyELISA Oxidized Protein Quantitation Kit provides a sensitive methodology for detection & quantitation of proteins modified by oxygen free radicals & other reactive species.
      Key Applications
      • ELISA
      Biological Information
      Species Reactivity
      • Mouse
      • Human
      • Rat
      • Pig
      • Horse
      • Rabbit
      • Guinea Pig
      • Hamster
      • Non-human Primate
      • Canine
      Analytes Available
      • Oxidized Protein
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C for up to 1 year after date of receipt.
      Packaging Information
      Material Size192 tests
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Diacetylbis(N(4)-methylthiosemicarbazonato) copper(II) (CuII(atsm)) protects against peroxynitrite-induced nitrosative damage and prolongs survival in amyotrophic lateral sclerosis mouse model.
      Cynthia P W Soon,Paul S Donnelly,Bradley J Turner,Lin W Hung,Peter J Crouch,Nicki A Sherratt,Jiang-Li Tan,Nastasia K-H Lim,Linh Lam,Laura Bica,Sinchun Lim,James L Hickey,Julia Morizzi,Andrew Powell,David I Finkelstein,Janetta G Culvenor,Colin L Masters,James Duce,Anthony R White,Kevin J Barnham,Qiao-Xin Li
      The Journal of biological chemistry  286  2011

      Show Abstract
      22033929 22033929

      Posters

      Title
      Poster: Methods For The Detection of Oxidative Stress