492899 Nuclease, Staphylococcus aureus

492899
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      492899-15KU
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          Plastic ampoule 15 ku
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          Description
          OverviewNative Stapylococcus aureus nuclease. Cleaves RNA and DNA to produce oligo- and mononucleotides with terminal 3′-phosphate groups. Also used to convert standard rabbit reticulocyte cell-free extracts (lysate) into mRNA-dependent protein synthesis systems. Note: 1 KU = 1000 units.
          Catalogue Number492899
          Brand Family Calbiochem®
          References
          ReferencesKrupp, G., and Gross, H.J. 1979. Nucleic Acids Res. 6, 3481.
          Product Information
          CAS number9013-53-0
          Unit of DefinitionOne unit is defined as the amount of enzyme that will release a sufficient amount of acid-soluble oligonucleotides to produce an absorbance increase of 1 at 260 nm in 30 min at 37°C.
          EC number3.1.31.1
          FormLyophilized off-white solid
          Hygroscopic Hygroscopic
          Applications
          Biological Information
          Specific Activity≥15,000 units/mg protein
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Hygroscopic Hygroscopic
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          492899

          References

          Reference overview
          Krupp, G., and Gross, H.J. 1979. Nucleic Acids Res. 6, 3481.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision14-April-2008 JSW
          DescriptionNative Stapylococcus aureus nuclease. Cleaves RNA and DNA to produce oligo- and mononucleotides with terminal 3′-phosphate groups. Also used to convert standard rabbit reticulocyte cell-free extracts (lysate) into mRNA-dependent protein synthesis systems. Note: 1 KU = 1000 units.
          FormLyophilized off-white solid
          CAS number9013-53-0
          EC number3.1.31.1
          Specific activity≥15,000 units/mg protein
          Unit definitionOne unit is defined as the amount of enzyme that will release a sufficient amount of acid-soluble oligonucleotides to produce an absorbance increase of 1 at 260 nm in 30 min at 37°C.
          SolubilityAqueous buffers (1 mg/ml)
          Storage +2°C to +8°C
          Hygroscopic
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions.
          Toxicity Standard Handling
          ReferencesKrupp, G., and Gross, H.J. 1979. Nucleic Acids Res. 6, 3481.