71003 NovaTaq™ DNA Polymerase

71003
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      71003-3
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          Glass bottle 100 u
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          71003-4
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              Glass bottle 500 u
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              71003-5
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                  Glass bottle 2500 u
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                  Description
                  OverviewNovaTaq™ DNA Polymerase* is a premium quality, recombinant form of Thermus aquaticus DNA polymerase that is licensed for PCR. This thermostable enzyme is suitable for a wide range of PCR applications. To ensure the highest purity and reproducible perfomance, each preparation is extensively tested in a variety of quality control assays. NovaTaq DNA Polymerase has an integral 5′→3′ exonuclease and lacks 3′→5′ exonuclease activity. The enzyme generates PCR products with 3′-dA overhangs, suitable for cloning with Novagen Perfectly Blunt®, AccepTor™, and LIC Vector Kits. Each item also includes optimized 10X NovaTaq Buffer with 15 mM MgCl2 for routine amplification conditions, plus separate vials of 10X NovaTaq Buffer without MgCl2, and 25 mM MgCl2 to enable convenient optimization of Mg2+ concentration.
                  Source Recombinant Thermus aquaticus DNA polymerase expressed in E. coli
                  Concentration 5.0 U/µl
                  Purity > 95% homogeneous by SDS-PAGE
                  Endonuclease None detected
                  Amplification effiency Functional PCR
                  Storage –20°C

                  Catalogue Number71003 Brand Family Novagen®

                  References
                  Product Information
                  Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 74°C, in a reaction containing 25 mM TAPS (tris-[hydroxymethyl]-methyl-amino-propane-sulfonic acid, sodium salt), pH 9.3 at 25°C, 50 mM KCl, 2 mM MgCl₂, 1 mM 2-mercaptoethanol, 0.2 mM dATP, dGTP, and dTTP, 0.1 µM [α-<Sup>32</Sup>P]dCTP, and activated salmon sperm DNA.
                  100 U, 500 U, or 5 × 500 UNovaTaq DNA Polymerase
                  1 or 2 or 7 × 1.5 ml10X NovaTaq Buffer with MgCl₂
                  1 or 2 or 7 × 1.5 ml10X NovaTaq Buffer without MgCl₂
                  1 or 2 or 7 × 1.5 ml25 mM MgCl₂
                  DeclarationPurchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
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                  Safety Information according to GHS
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                  Product Usage Statements
                  Storage and Shipping Information
                  Ship Code Dry Ice Only
                  Toxicity Multiple Toxicity Values, refer to MSDS
                  Storage -20°C
                  Do not freeze Ok to freeze
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                  Supplemental Information
                  Specifications

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                  Categories

                  Life Science Research > Genomic Analysis > DNA Preparation & Cloning > PCR & RT-PCR Kits & Reagents