480012 NDSB Set - Calbiochem

480012
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      480012-1SET
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          Glass bottle 1 set
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          Description
          OverviewContains the following non-detergent sulfobetaines: 5 g of NDSB-195 (Cat. No. 480001), 25 g of NDSB-201 (Cat. No. 480005), and 5 g of NDSB-256 (Cat. No. 480010). Supplied with a data sheet.
          Catalogue Number480012
          Brand Family Calbiochem®
          References
          ReferencesGoldberg, M.E., et al. 1996. Folding and Design 1, 21.
          Goldberg, M.E., et al. 1995. Folding & Design 1, 21.
          Vuillard, L., et al. 1995. Biochem. J. 305, 337.
          Vuillard, L., et al. 1995. Anal. Biochem. 230, 290.
          Vuillard, L., et al. 1994. FEBS Lett. 353, 294.
          Product Information
          FormSolid
          Hygroscopic Hygroscopic
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          R PhraseR: 36/37/38

          Irritating to eyes, respiratory system and skin.
          S PhraseS: 26-36

          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Irritant
          Storage +15°C to +30°C
          Hygroscopic Hygroscopic
          Do not freeze Ok to freeze
          Special InstructionsStore lyophilized products at room temperature. Following reconstitution, store in the refrigerator (4°C) or at room temperature (20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          480012

          References

          Reference overview
          Goldberg, M.E., et al. 1996. Folding and Design 1, 21.
          Goldberg, M.E., et al. 1995. Folding & Design 1, 21.
          Vuillard, L., et al. 1995. Biochem. J. 305, 337.
          Vuillard, L., et al. 1995. Anal. Biochem. 230, 290.
          Vuillard, L., et al. 1994. FEBS Lett. 353, 294.

          Brochure

          Title
          Biological Detergents Brochure
          Detergents and Buffers Technical Bulletin
          Protein Purification and Detection Tools
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision11-August-2008 RFH
          DescriptionNon-detergent sulfobetaines (NDSBs) are zwitterionic compounds. Like zwittergents (zwitterionic detergents), NDSBs carry the sulfobetaine hydrophilic head group. However, unlike zwittergents, the hydrophobic group in NDSBs is too short for micellar formation even at concentrations as high as 1M. Hence, NDSBs do not behave like detergents. Because of their zwitterionic nature, NDSBs were first employed to neutralize electrostatic interactions without increasing the conductivity in native isoelectrofocusing gels. Recently they have found utility in various other applications including isolation of membrane proteins, purification of nuclear proteins and halophilic proteins, renaturation of chemically and thermally denatured proteins and protein crystallization. They are potentially useful in solubilization and renaturation of fusion proteins from inclusion bodies. It is hypothesized that the hydrophobic group, although short, interacts with the hydrophobic regions of the protein preventing aggregation during renaturation. Also, presumably, the contribution from the short hydrophobic groups combined with charge neutralization ability of the sulfobetaine group results in higher yields of membrane proteins.

          NDSBs do not interfere with enzymatic assays based on chromogenic substrates bearing nitrophenyl groups and do not inhibit the activities of β-galactosidase and alkaline phosphatase, the two enzymes tested thus far. NDSB-195 has the added advantage in that it does not absorb at 280 nm, making it compatible with protein purification procedures in which the protein concentrations are determined by absorbance measurements at 280 nm. Supplied with a data sheet.
          FormSolid
          SolubilityH₂O (50 mg/ml)
          Storage +15°C to +30°C
          Hygroscopic
          Do Not Freeze Ok to freeze
          Special InstructionsStore lyophilized products at room temperature. Following reconstitution, store in the refrigerator (4°C) or at room temperature (20°C).
          Toxicity Irritant
          ReferencesGoldberg, M.E., et al. 1996. Folding and Design 1, 21.
          Goldberg, M.E., et al. 1995. Folding & Design 1, 21.
          Vuillard, L., et al. 1995. Biochem. J. 305, 337.
          Vuillard, L., et al. 1995. Anal. Biochem. 230, 290.
          Vuillard, L., et al. 1994. FEBS Lett. 353, 294.

          Related Products & Applications

          Related Products

          Catalog Number Description  
          480001 NDSB-195 - CAS 160255-06-1 - Calbiochem Show Pricing & Availability
          480005 NDSB-201 - CAS 15471-17-7 - Calbiochem Show Pricing & Availability
          480010 NDSB-256 - CAS 81239-45-4 - Calbiochem Show Pricing & Availability

          Related Products

          Catalog Number Description  
          480001 NDSB-195 - CAS 160255-06-1 - Calbiochem Show Pricing & Availability
          480005 NDSB-201 - CAS 15471-17-7 - Calbiochem Show Pricing & Availability
          480010 NDSB-256 - CAS 81239-45-4 - Calbiochem Show Pricing & Availability

          Categories

          Life Science Research > Inhibitors and Biochemicals > Biochemicals > Detergents > Non-Detergent Sulfobetaines