AAMT001 Mitochondrial Complex I Activity Assay Kit

AAMT001
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      AAMT001-1KIT
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          Glass bottle 96 rxn
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          Product Description

          The Mitochondrial Complex I Activity Assay Kit (Cat. No. AAMT001-1KIT) is used to determine the activity of mitochondrial OXPHOS Complex I (NADH dehydrogenase, E.C. 1.6.5.3). The enzyme complex is immunocaptured within the wells of the microplate and activity is determined by following the oxidation of NADH to NAD+ and the simultaneous reduction of a dye which leads to increased absorbance at 450 nm.



          See Figure 1 at the bottom of this page for the Complex I activity reaction scheme. The oxidation of NADH is linked to the reduction of a dye which has increased absorbance at 450 nm and so appears yellow in color.



          Panel Components

          25 ml 	Buffer, (72560-25ML)
          10 ml	10X Blocking Solution, (72561-10ML)
          1 ml	Detergent, (72562-1ML)
          1 ea        20X NADH (40 mM), (72563-1EA)
          1 ea        100X Dye, (72564-1EA)
          1 ea	Microplate, (72565-1EA)
          

          * This kit contains sufficient materials for 96 measurements (one 96-well microplate).



          Storage: Store all components at 4°C. After hydration of the 20X NADH and 100X dye with dH20, both of these components should be stored be aliquotted and stored at –80°C, as described in the FAQ section of TB554.



          This kit contains a 96-well microplate with a monoclonal antibody pre-bound to the wells of the microplate. This plate can be broken into 12 separate 8-well strips for convenience; therefore the plate can be used for up to 12 separate experiments.

          The Mitochondrial Complex I Activity Assay Kit contains sufficient materials for 96 measurements (one 96-well microplate).

          Catalogue NumberAAMT001
          Brand Family Novagen®
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          Ship Code Blue Ice Only
          Toxicity Irritant
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
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