475989 MTT - CAS 298-93-1 - Calbiochem

475989
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical FormulaCAS #
      C₁₈H₁₆BrN₅S 298-93-1

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      475989-10GM
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          Plastic ampoule 10 gm
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          475989-1GM
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              Glass bottle 1 gm
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              Description
              OverviewMembrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan. Spectrophotometric measurement of MTT-formazan at 540 or 570 nm allows quantitation of cell viability. Used as a direct indicator of cytotoxicity (such as for screening cancer drugs) and apoptosis. An electron acceptor used for studying NADP-dependent dehydrogenases.
              Catalogue Number475989
              Brand Family Calbiochem®
              Synonyms3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium Bromide, Thiazole Blue
              References
              ReferencesLiu, Y., et al. 1997. J. Neurochem. 69, 581.
              Abdallah, M.A. 1980. Eur. J. Biochem. 112, 331.
              Product Information
              CAS number298-93-1
              ATP CompetitiveN
              FormYellow solid
              Hill FormulaC₁₈H₁₆BrN₅S
              Chemical formulaC₁₈H₁₆BrN₅S
              ReversibleN
              Structure formula ImageStructure formula Image
              Applications
              Biological Information
              Primary TargetUsed as a direct indicator of cytotoxicity and apoptosis.
              Purity≥98% by argentometry
              Physicochemical Information
              Cell permeableY
              ContaminantsSulfated ash: ≤0.5%; heavy metals: ≤0.001%
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              RTECSXF8060000
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Ambient Temperature Only
              Toxicity Harmful & Carcinogenic / Teratogenic
              Storage +15°C to +30°C
              Do not freeze Ok to freeze
              Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C.). Stock solutions are stable for up to 1 month at -20°C.
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              SDS

              Title

              Safety Data Sheet (SDS) 

              Certificates of Analysis

              TitleLot Number
              475989

              References

              Reference overview
              Liu, Y., et al. 1997. J. Neurochem. 69, 581.
              Abdallah, M.A. 1980. Eur. J. Biochem. 112, 331.
              Data Sheet

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision28-January-2008 JSW
              Synonyms3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium Bromide, Thiazole Blue
              DescriptionMembrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan. Spectrophotometric measurement of MTT-formazan at 540 or 570 nm allows quantitation of cell viability. Used as a direct indicator of cytotoxicity (such as for screening cancer drugs) and apoptosis. An electron acceptor used for studying NADP-dependent dehydrogenases.
              FormYellow solid
              CAS number298-93-1
              RTECSXF8060000
              Chemical formulaC₁₈H₁₆BrN₅S
              Structure formulaStructure formula
              Purity≥98% by argentometry
              ContaminantsSulfated ash: ≤0.5%; heavy metals: ≤0.001%
              SolubilityPBS (2 mg/ml)
              Storage +15°C to +30°C
              Do Not Freeze Ok to freeze
              Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C.). Stock solutions are stable for up to 1 month at -20°C.
              Toxicity Harmful & Carcinogenic / Teratogenic
              ReferencesLiu, Y., et al. 1997. J. Neurochem. 69, 581.
              Abdallah, M.A. 1980. Eur. J. Biochem. 112, 331.