PRCYTOMAG-40K MILLIPLEX MAP Non-Human Primate Cytokine Magnetic Bead Panel - Immunology Multiplex Assay

PRCYTOMAG-40K

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Overview

Replacement Information

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This panel is available for custom premix. Talk to your Protein Specialist today!

Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
G-CSF, GM-CSF, IFN-γ, IL-1ra, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12/23 (p40), IL-13, IL-15, IL-17, IL-18, MCP-1, MIP-1α, MIP-1β, sCD40L, TGF-α, TNF-α, VEGF NhP Mplex Luminex xMAP
Description
Catalogue NumberPRCYTOMAG-40K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Non-Human Primate Cytokine Magnetic Bead Panel - Immunology Multiplex Assay
OverviewTo identify specific cytokines involved in any inflammatory or immune response, it may be necessary to screen panels of cytokines, often requiring some level of automation and/or high throughput. Magnetic Beads can make the process of automation and high throughput screening easier with features such as walk-away washing. Advantages even outside automation include:
• More flexible plate and plate washer options
• Improved performance with turbid serum/plasma samples
• Assay results equivalent to non-magnetic beads
• Automated magnetic washing avoids many problems associated with vacuum filtration washing

Therefore, the MILLIPLEX MAP Non-Human Primate Cytokine / Chemokine Panel enables you to focus on the therapeutic potential of cytokines, as well as the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, dramatically improve productivity.
Background InformationThe immune system is an organized complex network of biological structures and processes that protect against disease. For example, cytokine and chemokines mediate interactions between cells directly, regulating target immune cell responses. These responses result in inflammation where the immune system attempts to eradicate foreign antigens and begin the healing process. Consequently, the inflammatory process plays a key protective role in immunity. In addition cytokine and chemokine research is essential in understanding the immune system and its multi-faceted response to most antigens, as well as disease states such as autoimmune disease, allergic reactions, sepsis and cancer.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: 8 - 15
  • Intra-assay: 2 - 5
Panel TypeNon-Magnetic Cytokines/Chemokines
Applications
ApplicationSimultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight or two-hour incubation assay; however, for best results an overnight incubation is recommended.
• This assay requires 25 µl of neat plasma or serum, or 25 μL cell culture supernatant per well.
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Non-human Primate
Analytes Available
  • G-CSF
  • GM-CSF
  • IFN-γ
  • IL-1ra
  • IL-1β
  • IL-2
  • IL-4
  • IL-5
  • IL-6
  • IL-8
  • IL-10
  • IL-12/23 (p40)
  • IL-13
  • IL-15
  • IL-17
  • IL-18
  • MCP-1
  • MIP-1α
  • MIP-1β
  • sCD40L
  • TGF-α
  • TNF-α
  • VEGF
Assay PanelEverything you need in a single kit.
Cross ReactivityThe multiplexed assay panel has been tested with samples from multiple non-human
primate species, please see protocol for details.
Accuracy
  • 70% - 101%
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivities for individual cytokines/chemokines
Standard Curve Range
  • IL-4: 4.9 - 20,000 pg/mL
  • IL-10, IL-18: 12.2 - 50,000 pg/mL
  • All other analytes: 2.4 - 10,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Non-Human Primate Cytokine Magnetic Bead Panel

MILLIPLEX MAP Non-Human Primate Cytokine Magnetic Bead Panel - Immunology Multiplex Assay SDS

Title

Safety Data Sheet (SDS) 

References

Reference overviewPub Med ID
The Lyme disease spirochete Borrelia burgdorferi induces inflammation and apoptosis in cells from dorsal root ganglia.
Ramesh, G; Santana-Gould, L; Inglis, FM; England, JD; Philipp, MT
Journal of neuroinflammation  10  88  2013

Show Abstract
23866773 23866773

Brochure

Title
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with EMD Millipore Immunoassay Platform Solutions (SigmaMillipore)
Analyte Quarterly Vol 1 2019
Protein Detection Platform and Services Brochure
The Power of Biomarker Analysis

Technical Info

Title
96 well Template 2 Up
96 well Template Single
PRCYTOMAG-40K xPonent Protocol Template Version 3.1
PRCYTOMAG-40K xPonent Protocol Template Version Magpix
PRCYTOMAG-40K xPonent Standard Template Version 3.1
PRCYTOMAG-40K xPonent Standard Template Version Magpix
Quality Control Ranges: MILLIPLEX® MAP Non-Human Primate Cytokine/Chemokine Kit
Quality Control Ranges: MILLIPLEX® MAP Non-Human Primate Cytokine/Chemokine Kit

Data Sheet

Title
Full Service Custom-built Assays

Posters

Title
MILLIPLEX MAP: Exercise, Signaling, and Your Brain (EMD)
MILLIPLEX® MAP Cell Signaling Wall Chart