MGAMMAG-300K MILLIPLEX MAP Mouse Immunoglobulin Isotyping Magnetic Bead Panel - Isotyping Multiplex Assay

MGAMMAG-300K

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Overview

Replacement Information
Replacement InformationThis product replaces Mouse Immunoglobulin Isotyping Kit, MGAM-300 in December of 2011 or while supplies last.

Special Offers

This panel is available for custom premix. Talk to your Protein Specialist today!

Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
IgG1, IgG2a, IgG2b, IgG3, IgA, IgM M Mplex Luminex xMAP
Description
Catalogue NumberMGAMMAG-300K
ReplacesMGAM-300
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Mouse Immunoglobulin Isotyping Magnetic Bead Panel - Isotyping Multiplex Assay
OverviewMILLIPLEX MAP Mouse Isotyping Magnetic Bead Panel, measuring mouse IgG subclasses (1, 2a, 2b, 3), IgM, and IgA, has been designed to enable you to measure both classes and subclasses accurately – all in one well. In addition this panel allows you to choose only those immunoglobulins you need for your experiments.
Background InformationProduced by plasma cells and lymphocytes, immunoglobulins (antibodies) are critically involved in immune response, attaching to antigens and playing a role in their destruction. Immunoglobulins (Ig) can be classified by isotype, classes that differ in function and antigen response due to structure variability. Six major isotypes have been identified in placental mammals: IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA – all found in normal individuals. Immunoglobulin-deficiency disorders, such as autoimmune disease, some GI conditions and malignancies, are characterized by specific isotype deficiencies or varying concentrations of one or more isotypes. Disease states can range from the absence of one isotype class or subclass to a total deficiency of immunoglobulin classes.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Intra-Assay 5 - 10%
  • Inter-Assay 14 - 17%
Panel TypeMAGNETIC Immune Response
Applications
ApplicationIsotyping Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple immunoglobulins (Ig) in murine serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This kit may be used for the analysis of IgG1, IgG2a, IgG2b, IgG3, IgA and IgM in mouse serum or tissue/cell lysate and culture supernatant samples.
• There are 2 one-hour room-temperature incubations.
• This assay requires 50 µl of 1:50,000 diluted plasma or serum, or 50 μL cell culture supernatant diluted to approximately 1μg/mL Ig per well.
Biological Information
SpecificityCross-reactivity between these antibodies and those against other immunoglobulins is non-detectable or negligible.
Species Reactivity
  • Mouse
Analytes Available
  • IgG1
  • IgG2a
  • IgG2b
  • IgG3
  • IgA
  • IgM
Accuracy
  • IgG1 96%
  • IgG2a 76%
  • IgG2b 98%
  • IgG3 85%
  • IgA 98%
  • IgM 100%
Physicochemical Information
Sensitivity
  • IgG1 0.7 ng/mL
  • IgG2a 0.67 ng/mL
  • IgG2b 0.18 ng/mL
  • IgG3 0.32 ng/mL
  • IgA 1.1 ng/mL
  • IgM 1.73 ng/mL
Standard Curve Range
  • IgG1: 2 – 1,500 ng/mL
  • IgG2a: 3 – 2,000 ng/mL
  • IgG2b: 1 – 900 ng/mL
  • IgG3: 2 – 1,500 ng/mL
  • IgA: 5 – 3,000 ng/mL
  • IgM: 2 – 1,500 ng/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 Well Plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Mouse Immunoglobulin Isotyping Magnetic Bead Panel

MILLIPLEX MAP Mouse Immunoglobulin Isotyping Magnetic Bead Panel - Isotyping Multiplex Assay SDS

Title

Safety Data Sheet (SDS) 

References

Reference overviewPub Med ID
Common tolerance mechanisms, but distinct cross-reactivities associated with gp41 and lipids, limit production of HIV-1 broad neutralizing antibodies 2F5 and 4E10.
Chen, Y; Zhang, J; Hwang, KK; Bouton-Verville, H; Xia, SM; Newman, A; Ouyang, YB; Haynes, BF; Verkoczy, L
Journal of immunology (Baltimore, Md. : 1950)  191  1260-75  2013

Show Abstract
23825311 23825311
Soluble receptor for advanced glycation end products alleviates nephritis in (NZB/NZW)F1 mice.
Lee, SW; Park, KH; Park, S; Kim, JH; Hong, SY; Lee, SK; Choi, D; Park, YB
Arthritis and rheumatism  65  1902-12  2013

Show Abstract
23553192 23553192
Forced miR-146a expression causes autoimmune lymphoproliferative syndrome in mice via downregulation of Fas in germinal center B cells.
Guo, Q; Zhang, J; Li, J; Zou, L; Zhang, J; Xie, Z; Fu, X; Jiang, S; Chen, G; Jia, Q; Li, F; Wan, Y; Wu, Y
Blood  121  4875-83  2013

Show Abstract
23645835 23645835

Brochure

Title
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with EMD Millipore Immunoassay Platform Solutions (SigmaMillipore)
Protein Detection Platform and Services Brochure
The Power of Biomarker Analysis

Technical Info

Title
96 well Template 2 Up
96 well Template Single
MGAMMAG-300K xPonent Protocol Template Version Magpix
MGAMMAG-300K xPonent Protocol Template Version 3.1
MGAMMAG-300K xPonent Standard Template Version 3.1
MGAMMAG-300K xPonent Standard Template Version Magpix
Quality Control Ranges: Milliplex Mouse Immunoglobulin Isotyping Kit and Magnetic Bead Panel

Data Sheet

Title
Full Service Custom-built Assays