444155 MDMX Antagonist, SJ-172550 - CAS 431979-47-4 - Calbiochem

444155
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical FormulaCAS #
      C₂₂H₂₁ClN₂O₅ 431979-47-4

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      444155-10MG
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          Glass bottle 10 mg
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          Description
          OverviewA cell-permeable phenyl-pyrazolylidene compound that competes with p53-derived peptide (aa 15-29) binding to MDMX in cell-free binding assays (EC50 = 2.3 µM) in a reversible manner and effectively disrupts cellular p53-MDMX interaction (IC50 <20 µM in C33A, HER, and Weri1 cultures), while exhibiting much weaker affinity toward MDM2 (EC50 = 26 µM in p53 peptide competition assays). MDMX inhibition by 20 µM SJ-172550 and MDM2 inhibition by 5 µM Nutlin-3a (Cat. Nos. 444143 & 444151) are both shown to result in reduced proliferation and increased cellular caspase-3 in cultured cells, however, only MDM2, but not MDMX, inhibition results in a significant p53 protein level upregulation.
          Catalogue Number444155
          Brand Family Calbiochem®
          SynonymsMethyl-2-(2-chloro-6-ethoxy-4-(E)-(3-methyl-5-oxo-1-phenyl-pyrazol-4-ylidene)methyl)phenoxy)acetate, MDMX Inhibitor II
          References
          ReferencesReed, D., et al. 2010. J. Biol. Chem. 285, 10786.
          Product Information
          CAS number431979-47-4
          FormOrange solid
          Hill FormulaC₂₂H₂₁ClN₂O₅
          Chemical formulaC₂₂H₂₁ClN₂O₅
          Structure formula ImageStructure formula Image
          Applications
          Biological Information
          Purity≥95% by NMR
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 6 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          444155

          References

          Reference overview
          Reed, D., et al. 2010. J. Biol. Chem. 285, 10786.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision26-September-2012 JSW
          SynonymsMethyl-2-(2-chloro-6-ethoxy-4-(E)-(3-methyl-5-oxo-1-phenyl-pyrazol-4-ylidene)methyl)phenoxy)acetate, MDMX Inhibitor II
          DescriptionA cell-permeable phenyl-pyraz-olylidene compound that competes with p53-derived peptide (aa 15-29) binding to MDMX in cell-free binding assays (EC50 = 2.3 µM) in a reversible manner and effectively disrupts cellular p53-MDMX interaction (IC50 <20 µM in C33A, HER, and Weri1 cultures), while exhibiting much weaker affinity toward MDM2 (EC50 = 26 µM in p53 peptide competition assays). MDMX inhibition by 20 µM SJ-172550 and MDM2 inhibition by 5 µM Nutlin-3a (Cat. Nos. 444143 & 444151) are both shown to result in reduced proliferation and increased cellular caspase-3 in cultured cells, however, only MDM2, but not MDMX, inhibition results in a significant p53 protein level upregulation.
          FormOrange solid
          Intert gas (Yes/No) Packaged under inert gas
          CAS number431979-47-4
          Chemical formulaC₂₂H₂₁ClN₂O₅
          Structure formulaStructure formula
          Purity≥95% by NMR
          SolubilityDMSO (50 mg/ml)
          Storage +2°C to +8°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 6 months at -20°C.
          Toxicity Standard Handling
          ReferencesReed, D., et al. 2010. J. Biol. Chem. 285, 10786.