431525 L-Leucine Dehydrogenase, Bacillus cereus

431525
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      431525-100U
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          Plastic ampoule 100 u
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          Description
          OverviewNative L-leucine dehydrogenase from Bacillus cereus. Catalyzes the NAD+-dependent conversion of L-leucine to α-ketoisocaproate. Suitable for the production of L-tertiary leucine amino acids.
          pH optimum 10.5-10.8 (L-leucine substrate) and 9.4 (α-ketoisocaproate substrate), temperature optimum 65°C.
          Catalogue Number431525
          Brand Family Calbiochem®
          SynonymsL-LeuDH, L-Leucine:NAD+-oxidoreductase
          References
          ReferencesKärst, U., et al. 1986. FEMS Microbiol. Lett. 37, 335.
          Schütte, H., et al. 1985. Appl. Microbiol. Biotechnol. 22, 306.
          Product Information
          CAS number9082-71-7
          Activity≥50 units/ml
          Unit of DefinitionOne unit is defined as the amount of enzyme that will convert 1.0 µmol L-leucine to α-ketoisocaproate in the presence of NAD<sup>+</sup> per min at 30°C, pH 10.7.
          EC number1.4.1.9
          FormLiquid
          FormulationIn 100 mM NaCl, 10 mM potassium phosphate buffer, 50% glycerol, pH 7.5.
          Applications
          Biological Information
          Specific Activity≥10 units/mg protein
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          431525

          References

          Reference overview
          Kärst, U., et al. 1986. FEMS Microbiol. Lett. 37, 335.
          Schütte, H., et al. 1985. Appl. Microbiol. Biotechnol. 22, 306.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision05-August-2008 RFH
          SynonymsL-LeuDH, L-Leucine:NAD+-oxidoreductase
          DescriptionNative L-leucine dehydrogenase from Bacillus cereus. Purified enzyme that catalyzes the NAD-dependent conversion of L-leucine to α-ketoisocaproate. Suitable for the production of L-tertiary leucine amino acids. Has an optimal pH of 10.5-10.8 for L-leucine substrate and 9.4 for α-ketoisocaproate substrate. Has an optimal temperature of 65°C.
          FormLiquid
          FormulationIn 100 mM NaCl, 10 mM potassium phosphate buffer, 50% glycerol, pH 7.5.
          CAS number9082-71-7
          EC number1.4.1.9
          Specific activity≥10 units/mg protein
          Activity≥50 units/ml
          Unit definitionOne unit is defined as the amount of enzyme that will convert 1.0 µmol L-leucine to α-ketoisocaproate in the presence of NAD+ per min at 30°C, pH 10.7.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesKärst, U., et al. 1986. FEMS Microbiol. Lett. 37, 335.
          Schütte, H., et al. 1985. Appl. Microbiol. Biotechnol. 22, 306.