419700 Isopeptidase T, Rabbit

419700
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      419700-25UG
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          Plastic ampoule 25 μg
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          Description
          OverviewNative, rabbit isopeptidase T. A member of the higher molecular weight group of ubiquitin C-terminal hydrolases (UCHs) involved in the hydrolysis of isopeptide linkages of polyUb chains. Ipase-T activity is regulated by ubiquitin levels. Plays a major role in ubiquitin recycling and in the regulation of 26S proteasome activity. Recommended reaction buffer: 25 mM HEPES, 10 mM DTT, 25°C, pH 7.5. Pre-incubate the enzyme for 15 minutes with 10 mM DTT to achieve maximum activity.
          Catalogue Number419700
          Brand Family Calbiochem®
          SynonymsIpase-T
          References
          ReferencesDang, L.C., et al. 1998. Biochemistry 37, 1868.
          Melandri, F.D., et al. 1996. Biochemistry 35, 12893.
          Stein, R.L., et al. 1995. Biochemistry 34, 12616.
          Pickart, C.M., and Rose, I.A. 1986. J. Biol. Chem. 261, 10210.
          Product Information
          FormLiquid
          FormulationIn 150 mM NaCl, 50 mM HEPES, 1 mM DTT, pH 7.6.
          Applications
          Biological Information
          Biological activityFully functional as measured using 5 nM Ipase-T and 1 µM Ub-AMC (Cat. No. 662075)
          Purity≥95% by SDS-PAGE
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          419700

          References

          Reference overview
          Dang, L.C., et al. 1998. Biochemistry 37, 1868.
          Melandri, F.D., et al. 1996. Biochemistry 35, 12893.
          Stein, R.L., et al. 1995. Biochemistry 34, 12616.
          Pickart, C.M., and Rose, I.A. 1986. J. Biol. Chem. 261, 10210.

          Brochure

          Title
          Proteases Technical Bulletin
          Proteasomes Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision24-July-2008 RFH
          SynonymsIpase-T
          DescriptionNative, rabbit isopeptidase T. A member of the higher molecular weight group of ubiquitin C-terminal hydroxylases (UCHs) involved in the hydrolysis of isopeptide linkages of polyUb chains. Ipase-T activity is regulated by ubiquitin levels. Plays a major role in ubiquitin recycling and in the regulation of 26S proteasome activity.
          FormLiquid
          FormulationIn 150 mM NaCl, 50 mM HEPES, 1 mM DTT, pH 7.6.
          Recommended reaction conditions25 mM HEPES, 10 mM DTT, 25°C, pH 7.5. Pre-incubate the enzyme for 15 min with 10 mM DTT to achieve maximum activity.
          Purity≥95% by SDS-PAGE
          Biological activityFully functional as measured using 5 nM Ipase-T and 1 µM Ub-AMC (Cat. No. 662075)
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesDang, L.C., et al. 1998. Biochemistry 37, 1868.
          Melandri, F.D., et al. 1996. Biochemistry 35, 12893.
          Stein, R.L., et al. 1995. Biochemistry 34, 12616.
          Pickart, C.M., and Rose, I.A. 1986. J. Biol. Chem. 261, 10210.