400052 Hygromycin B, Streptomyces sp., Sterile-Filtered Solution in PBS, Cell Culture-Tested - CAS 31282-04-9 - Calbiochem

400052
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical FormulaCAS #
      C₂₀H₃₇N₃O₁₃ 31282-04-9

      Pricing & Availability

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      400052-20ML
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          Alu drum 20 ml
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          400052-50ML
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              Alu drum 50 ml
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              400052-5ML
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                  Alu drum 5 ml
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                  Description
                  OverviewSterile liquid formulation of Cat. No. 400050.
                  Catalogue Number400052
                  Brand Family Calbiochem®
                  References
                  ReferencesSugimoto, K., et al. 1994. Plant J. 5, 863.
                  Lee, M.G., and van der Ploeg, L.H. 1991. Gene 105, 255.
                  Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 34, 1915.
                  Cullen, D., et al. 1987. Gene 57, 21.
                  Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.
                  Santerre, R.F., et al. 1984. Gene 30, 147.
                  Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.
                  Product Information
                  CAS number31282-04-9
                  FormLiquid
                  Formulation50 mg/ml active antibiotic in sterile-filtered PBS, pH 7.2.
                  Hill FormulaC₂₀H₃₇N₃O₁₃
                  Chemical formulaC₂₀H₃₇N₃O₁₃
                  Structure formula ImageStructure formula Image
                  Applications
                  ApplicationHygromycin B is an aminoglycoside antibiotic that inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Inhibits protein synthesis by interfering with the translocation of the 70S ribosome and causes misreading of mRNA.
                  Biological Information
                  Biological activityPotency: ≥1000 U/mg
                  Purity≥85% by HPLC and TLC
                  Physicochemical Information
                  Dimensions
                  Materials Information
                  Toxicological Information
                  Safety Information according to GHS
                  RTECSWK2130000
                  Safety Information
                  R PhraseR: 26/27/28-37/38-41

                  Very toxic by inhalation, in contact with skin and if swallowed.
                  Irritating to respiratory system and skin.
                  Risk of serious damage to eyes.
                  S PhraseS: 23-26-36/37/39-45

                  Do not breathe fumes.
                  In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
                  Wear suitable protective clothing, gloves and eye/face protection.
                  In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
                  Product Usage Statements
                  Storage and Shipping Information
                  Ship Code Ambient Temperature Only
                  Toxicity Highly Toxic & Carcinogenic / Teratogenic
                  Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
                  Storage +2°C to +8°C
                  Do not freeze Ok to freeze
                  Packaging Information
                  Transport Information
                  Supplemental Information
                  Specifications

                  Documentation

                  SDS

                  Title

                  Safety Data Sheet (SDS) 

                  Certificates of Analysis

                  TitleLot Number
                  400052

                  References

                  Reference overview
                  Sugimoto, K., et al. 1994. Plant J. 5, 863.
                  Lee, M.G., and van der Ploeg, L.H. 1991. Gene 105, 255.
                  Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 34, 1915.
                  Cullen, D., et al. 1987. Gene 57, 21.
                  Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.
                  Santerre, R.F., et al. 1984. Gene 30, 147.
                  Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.

                  Brochure

                  Title
                  Antibiotics Profiler
                  Hygromycin B Technical Bulletin
                  The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression (EMD)

                  Technical Info

                  Title
                  Molecular Biology Grade Reagents
                  Data Sheet

                  Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

                  Revision04-October-2007 RFH
                  DescriptionHygromycin B is an aminoglycoside antibiotic that inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Inhibits protein synthesis by interfering with the translocation of the 70S ribosome and causes misreading of mRNA. Resistance to hygromycin B is conferred by a gene whose product, hygromycin B phospho-transferase that phosphorylates and inactivates the drug. Hygromycin B has been used to select mutants carrying the hygromycin B resistance gene in a wide variety of cells including bacterial, protozoal, yeast, fungal, plant, and mammalian cells. Typical concentrations used for mammalian cell selection are between 0.05 to 1 mg/ml. The appropriate concentration for use must be determined empirically for each cell type.
                  FormLiquid
                  Formulation50 mg/ml active antibiotic in sterile-filtered PBS, pH 7.2.
                  CAS number31282-04-9
                  RTECSWK2130000
                  Chemical formulaC₂₀H₃₇N₃O₁₃
                  Structure formulaStructure formula
                  Purity≥85% by HPLC and TLC
                  Biological activityPotency: ≥1000 U/mg
                  Storage +2°C to +8°C
                  Do Not Freeze Ok to freeze
                  Toxicity Highly Toxic & Carcinogenic / Teratogenic
                  Merck USA index14, 4852
                  ReferencesSugimoto, K., et al. 1994. Plant J. 5, 863.
                  Lee, M.G., and van der Ploeg, L.H. 1991. Gene 105, 255.
                  Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 34, 1915.
                  Cullen, D., et al. 1987. Gene 57, 21.
                  Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.
                  Santerre, R.F., et al. 1984. Gene 30, 147.
                  Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.

                  Related Products & Applications

                  Alternative Products

                  Catalog Number Description  
                  400051 Hygromycin B, Streptomyces sp. - CAS 31282-04-9 - Calbiochem Show Pricing & Availability
                  400053 Hygromycin B, Streptomyces sp., Sterile-Filtered Solution in 25 mM HEPES, Cell Culture-Tested - CAS 31282-04-9 - Calbiochem Show Pricing & Availability

                  Categories

                  Life Science Research > Inhibitors and Biochemicals > Biochemicals > Antibiotics > Antimicrobial Agents
                  Life Science Research > Cell Culture and Systems > Antibiotics & Selection Agents