400053 Hygromycin B, Streptomyces sp., Sterile-Filtered Solution in 25 mM HEPES, Cell Culture-Tested - CAS 31282-04-9 - Calbiochem

Hygromycin B is an aminoglycoside antibiotic that inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Inhibits protein synthesis by interfering with the translocation of the 70S ribosome and causes misreading of mRNA.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
400053
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical FormulaCAS #
      C₂₀H₃₇N₃O₁₃ 31282-04-9

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      400053-20ML
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          		 Alu drum 20 ml
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          400053-5ML
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              		 Alu drum 5 ml
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              Description
              OverviewSterile liquid formulation of Cat. No. 400050.
              Catalogue Number400053
              Brand Family Calbiochem®
              References
              ReferencesSugimoto, K., et al. 1994. Plant J. 5, 863.
              Lee, M.G., and van der Ploeg, L.H. 1991. Gene 105, 255.
              Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 34, 1915.
              Cullen, D., et al. 1987. Gene 57, 21.
              Santerre, R.F., et al. 1984. Gene 30, 147.
              Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.
              Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.
              Product Information
              CAS number31282-04-9
              FormLiquid
              Formulation50 mg/ml active antibiotic in sterile-filtered 25 mM HEPES, pH 7.2.
              Hill FormulaC₂₀H₃₇N₃O₁₃
              Chemical formulaC₂₀H₃₇N₃O₁₃
              Structure formula ImageStructure formula Image
              Applications
              ApplicationHygromycin B is an aminoglycoside antibiotic that inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Inhibits protein synthesis by interfering with the translocation of the 70S ribosome and causes misreading of mRNA.
              Biological Information
              Purity≥90% by HPLC
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              RTECSWK2130000
              Safety Information
              R PhraseR: 26/27/28-37/38-41

              Very toxic by inhalation, in contact with skin and if swallowed.
              Irritating to respiratory system and skin.
              Risk of serious damage to eyes.
              S PhraseS: 23-26-36/37/39-45

              Do not breathe fumes.
              In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
              Wear suitable protective clothing, gloves and eye/face protection.
              In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Ambient Temperature Only
              Toxicity Highly Toxic & Carcinogenic / Teratogenic
              Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
              Storage +2°C to +8°C
              Do not freeze Ok to freeze
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              SDS

              Title

              Safety Data Sheet (SDS) 

              Certificates of Analysis

              TitleLot Number
              400053

              References

              Reference overview
              Sugimoto, K., et al. 1994. Plant J. 5, 863.
              Lee, M.G., and van der Ploeg, L.H. 1991. Gene 105, 255.
              Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 34, 1915.
              Cullen, D., et al. 1987. Gene 57, 21.
              Santerre, R.F., et al. 1984. Gene 30, 147.
              Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.
              Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.

              Brochure

              Title
              Antibiotics Profiler
              Hygromycin B Technical Bulletin
              Data Sheet

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision06-September-2007 JSW
              DescriptionHygromycin B is an aminoglycoside antibiotic, inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Inhibits protein synthesis by interfering with the translocation of the 70S ribosome and causes misreading of mRNA. Resistance to hygromycin B is conferred by a gene whose product, hygromycin B phospho-transferase that phosphorylates and inactivates the drug. Hygromycin B has been used to select mutants carrying the hygromycin B resistance gene in a wide variety of cells including bacterial, protozoal, yeast, fungal, plant, and mammalian cells. Typical concentrations used for mammalian cell selection are between 0.05 to 1 mg/ml. The appropriate concentration for use must be determined empirically for each cell type.
              FormLiquid
              Formulation50 mg/ml active antibiotic in sterile-filtered 25 mM HEPES, pH 7.2.
              CAS number31282-04-9
              RTECSWK2130000
              Chemical formulaC₂₀H₃₇N₃O₁₃
              Structure formulaStructure formula
              Purity≥90% by HPLC
              Storage +2°C to +8°C
              Do Not Freeze Ok to freeze
              Toxicity Highly Toxic & Carcinogenic / Teratogenic
              Merck USA index14, 4852
              ReferencesSugimoto, K., et al. 1994. Plant J. 5, 863.
              Lee, M.G., and van der Ploeg, L.H. 1991. Gene 105, 255.
              Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 34, 1915.
              Cullen, D., et al. 1987. Gene 57, 21.
              Santerre, R.F., et al. 1984. Gene 30, 147.
              Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.
              Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.

              Related Products & Applications

              Alternative Products

              Catalog Number Description  
              400051 Hygromycin B, Streptomyces sp. - CAS 31282-04-9 - Calbiochem Show Pricing & Availability
              400052 Hygromycin B, Streptomyces sp., Sterile-Filtered Solution in PBS, Cell Culture-Tested - CAS 31282-04-9 - Calbiochem Show Pricing & Availability

              Categories

              Life Science Research > Inhibitors and Biochemicals > Biochemicals > Antibiotics > Antimicrobial Agents
              Life Science Research > Cell Culture and Systems > Antibiotics & Selection Agents