400050 | Hygromycin B, Streptomyces sp., Cell Culture-Tested - CAS 31282-04-9 - Calbiochem

400050
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical FormulaCAS #
      C₂₀H₃₇N₃O₁₃ 31282-04-9

      Pricing & Availability

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      400050-1.0GM
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          Glass bottle 1 gm
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          400050-100MG
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              Alu drum 100 mg
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              400050-500MG
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                  Alu drum 500 mg
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                  400050-5GM
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                      Alu drum 5 gm
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                      Description
                      OverviewUnique aminoglycoside antibiotic that inhibits the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells. Inhibits protein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA. Hph, a gene from E. coli, encodes resistance to hygromycin B and can be isolated and cloned by recombinant DNA techniques. This hygromycin B-resistance gene is particularly useful for the identification or selection of recombinant clones in a variety of cell types. Hygromycin B penetrates cells that have been permeabilized by virus infection and can act as an effective antiviral agent. Working concentration: 50 µg/ml - 1 mg/ml for mammalian cell selection.
                      Potency: ≥1000 µ/mg solid.
                      Catalogue Number400050
                      Brand Family Calbiochem®
                      References
                      ReferencesDean, N. 1995. Proc. Natl. Acad. Sci. USA 92, 1287.
                      Hubbard, S. C., et al. 1994. J. Biol. Chem. 269, 3717.
                      Rikkerink, E. H., et al. 1994. Current Genetics 25, 202.
                      Sugimoto, K., et al. 1994. Plant J. 5, 863.
                      MacIntyre, G., et al. 1992. Adv. Exp. Med. Biol. 276, 67.
                      Bilang, R., et al. 1991. Gene 100, 247.
                      Lee, M.G-S., and Van der Ploeg, L. 1991. Gene 105, 255.
                      Leslie, J.F., and Dickman, M.B. 1991. Applied Environ. Microbiol. 57, 1423.
                      MacIntyre, G., et al. 1991. Antimicrob. Agents Chemother. 35, 2125.
                      Zhou, J., et al. 1991. Gene 107, 307.
                      Bulte, L., and Bennoun, P. 1990. Current Genetics 18, 155.
                      Giordano, T.J., and McAllister, W.T. 1990. Gene 88, 285.
                      Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 24, 1915.
                      Carrasco, L., et al. 1989. Pharmacol. Ther. 9, 311.
                      Crespi, C.L., et al. 1989. Carcinogenesis 10, 295.
                      Damm, B., et al. 1989. Mol. Gen. Genetics 217, 6.
                      Egelhoff, T.T., et al. 1989. Mol. Cell. Biol. 9, 1965.
                      Kronstad, J.W., et al. 1989. Gene 79, 97.
                      Perlin, D.S., et al. 1988. J. Biol. Chem. 263, 118.
                      Cullen, D., et al. 1987. Gene 57, 21.
                      Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.
                      Malpartida, F., et al. 1983. Biochem. Biophys. Res. Commun. 117, 6.
                      Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.
                      Product Information
                      CAS number31282-04-9
                      FormLight brown lyophilized solid
                      Hill FormulaC₂₀H₃₇N₃O₁₃
                      Chemical formulaC₂₀H₃₇N₃O₁₃
                      Structure formula ImageStructure formula Image
                      Applications
                      Application CommentsProvided as a light brown lyophilized solid. Not provided under sterile conditions. If a sterile solution is desired, working solutions of Hygromycin B in aqueous media can be passed through a 0.2 micron filter before use.
                      Biological Information
                      Biological activityThe activity of Hygromycin B is measured in a zonal inhibition assay using Bacillus subtilis. The activity is measured relative to a reference standard.
                      Purity≥90% by HPLC and TLC
                      Physicochemical Information
                      Dimensions
                      Materials Information
                      Toxicological Information
                      Safety Information according to GHS
                      RTECSWK2130000
                      Safety Information
                      R PhraseR: 26/27/28-37/38-41

                      Very toxic by inhalation, in contact with skin and if swallowed.
                      Irritating to respiratory system and skin.
                      Risk of serious damage to eyes.
                      S PhraseS: 22-26-36/37/39-45

                      Do not breathe dust.
                      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
                      Wear suitable protective clothing, gloves and eye/face protection.
                      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
                      Product Usage Statements
                      Storage and Shipping Information
                      Ship Code Ambient Temperature Only
                      Toxicity Highly Toxic & Carcinogenic / Teratogenic
                      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
                      Storage +2°C to +8°C
                      Do not freeze Ok to freeze
                      Special InstructionsAqueous stock solutions should be kept at concentrations of ≥50 mg/ml and can be stored at either 4°C or -20°C. Aqueous stock solutions (≥50 mg/ml) are stable for up to 6 months at 4°C or -20°C. Working solutions (< 2 mg/ml) are stable for up to 1 month at 4°C.

                      Note: This product is highly toxic. Read enclosed MSDS before handling.
                      Packaging Information
                      Transport Information
                      Supplemental Information
                      Specifications

                      Documentation

                      SDS

                      Title

                      Safety Data Sheet (SDS) 

                      Certificates of Analysis

                      TitleLot Number
                      400050

                      References

                      Reference overview
                      Dean, N. 1995. Proc. Natl. Acad. Sci. USA 92, 1287.
                      Hubbard, S. C., et al. 1994. J. Biol. Chem. 269, 3717.
                      Rikkerink, E. H., et al. 1994. Current Genetics 25, 202.
                      Sugimoto, K., et al. 1994. Plant J. 5, 863.
                      MacIntyre, G., et al. 1992. Adv. Exp. Med. Biol. 276, 67.
                      Bilang, R., et al. 1991. Gene 100, 247.
                      Lee, M.G-S., and Van der Ploeg, L. 1991. Gene 105, 255.
                      Leslie, J.F., and Dickman, M.B. 1991. Applied Environ. Microbiol. 57, 1423.
                      MacIntyre, G., et al. 1991. Antimicrob. Agents Chemother. 35, 2125.
                      Zhou, J., et al. 1991. Gene 107, 307.
                      Bulte, L., and Bennoun, P. 1990. Current Genetics 18, 155.
                      Giordano, T.J., and McAllister, W.T. 1990. Gene 88, 285.
                      Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 24, 1915.
                      Carrasco, L., et al. 1989. Pharmacol. Ther. 9, 311.
                      Crespi, C.L., et al. 1989. Carcinogenesis 10, 295.
                      Damm, B., et al. 1989. Mol. Gen. Genetics 217, 6.
                      Egelhoff, T.T., et al. 1989. Mol. Cell. Biol. 9, 1965.
                      Kronstad, J.W., et al. 1989. Gene 79, 97.
                      Perlin, D.S., et al. 1988. J. Biol. Chem. 263, 118.
                      Cullen, D., et al. 1987. Gene 57, 21.
                      Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.
                      Malpartida, F., et al. 1983. Biochem. Biophys. Res. Commun. 117, 6.
                      Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.

                      Brochure

                      Title
                      Antibiotics Profiler
                      Hygromycin B Technical Bulletin
                      Data Sheet

                      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

                      Revision26-September-2007 JSW
                      DescriptionHygromycin B, an aminoglycoside antibiotic, inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Specifically, it inhibits protein synthesis by interfering with translocation of the 70S ribosome and inducing misreading of the mRNA template (Dean, N., Gonzalez, A., et al., Rao, S.N., et al.). Hygromycin B has been used to select mutants in a wide variety of cells including bacteria (Bilang, R., et al., Salauze, D., et al.), protozoans (Lee, M.G-S., and Van der Ploeg, L.H.T.), yeast (Perlin, D.S., et al.), fungi (Cullen, D., et al., Kronstad, J.W., et al., Egelhoff, T.T., et al., Leslie, J.F., and Dickman, M.B., Bulte, L., and Bennoun, P.), plants (Dean, N., Damm, B., et al., Rikkerink, E. H., et al., Sugimoto, K., et al.), and mammalian cells (Crespi, C.L., et al., Giordano, T.J., and McAllister, W.T., Hubbard, S. C., et al.).

                      Resistance to Hygromycin B is conferred by a gene coding for a phosphotransferase that phosphorylates Hygromycin B, thereby inactivating it (Bilang, R., et al., Malpartida, F., et al.). Hygromycin B is known to selectively penetrate cells that have been rendered permeable by virus infection. This, combined with its potency in inhibiting translation, makes it an effective antiviral agent (MacIntyre, G., et al., Zhou, J., et al.).

                      The analytical data listed below will vary from lot to lot.

                      Appearance: Off-white to light brown solid
                      Bioassay: 1000 U/mg
                      Solubility: ≥100 mg/ml in H2O
                      Purity (HPLC): ≥90%
                      Cytotoxicity: Tested in HeLa and/or CHO cells
                      BackgroundHygromycin B, an aminoglycoside antibiotic, inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Specifically, it inhibits protein synthesis by interfering with translocation of the 70S ribosome and inducing misreading of the mRNA template. Hygromycin B has been used to select mutants in a wide variety of cells including bacteria, protozoans, yeast, fungi, plants, and mammalian cells. Resistance to Hygromycin B is conferred by a gene coding for a phosphotransferase that phosphorylates Hygromycin B, thereby inactivating it. Hygromycin B is known to selectively penetrate cells that have been rendered permeable by virus infection. This combined with its potency in inhibiting translation, makes it an effective antiviral agent.
                      FormLight brown lyophilized solid
                      Recommended reaction conditions
                      Hygromycin B is not provided under sterile conditions. If a sterile solution is desired, working solutions of Hygromycin B in aqueous media can be passed through a 0.2 µm filter before use. When using this product for mammalian cell selection the working concentration is generally between 50 µg/ml and 1 mg/ml. The appropriate concentration for use must be determined empirically for each cell type.
                      CAS number31282-04-9
                      RTECSWK2130000
                      Chemical formulaC₂₀H₃₇N₃O₁₃
                      Structure formulaStructure formula
                      Purity≥90% by HPLC and TLC
                      Biological activityThe activity of Hygromycin B is measured in a zonal inhibition assay using Bacillus subtilis. The activity is measured relative to a reference standard.
                      SolubilitydH₂O (≥100 mg/ml)
                      CommentsProvided as a light brown lyophilized solid. Not provided under sterile conditions. If a sterile solution is desired, working solutions of Hygromycin B in aqueous media can be passed through a 0.2 micron filter before use.
                      Storage +2°C to +8°C
                      Do Not Freeze Ok to freeze
                      Special InstructionsAqueous stock solutions should be kept at concentrations of ≥50 mg/ml and can be stored at either 4°C or -20°C. Aqueous stock solutions (≥50 mg/ml) are stable for up to 6 months at 4°C or -20°C. Working solutions (< 2 mg/ml) are stable for up to 1 month at 4°C.

                      Note: This product is highly toxic. Read enclosed MSDS before handling.
                      Toxicity Highly Toxic & Carcinogenic / Teratogenic
                      Merck USA index14, 4852
                      ReferencesDean, N. 1995. Proc. Natl. Acad. Sci. USA 92, 1287.
                      Hubbard, S. C., et al. 1994. J. Biol. Chem. 269, 3717.
                      Rikkerink, E. H., et al. 1994. Current Genetics 25, 202.
                      Sugimoto, K., et al. 1994. Plant J. 5, 863.
                      MacIntyre, G., et al. 1992. Adv. Exp. Med. Biol. 276, 67.
                      Bilang, R., et al. 1991. Gene 100, 247.
                      Lee, M.G-S., and Van der Ploeg, L. 1991. Gene 105, 255.
                      Leslie, J.F., and Dickman, M.B. 1991. Applied Environ. Microbiol. 57, 1423.
                      MacIntyre, G., et al. 1991. Antimicrob. Agents Chemother. 35, 2125.
                      Zhou, J., et al. 1991. Gene 107, 307.
                      Bulte, L., and Bennoun, P. 1990. Current Genetics 18, 155.
                      Giordano, T.J., and McAllister, W.T. 1990. Gene 88, 285.
                      Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 24, 1915.
                      Carrasco, L., et al. 1989. Pharmacol. Ther. 9, 311.
                      Crespi, C.L., et al. 1989. Carcinogenesis 10, 295.
                      Damm, B., et al. 1989. Mol. Gen. Genetics 217, 6.
                      Egelhoff, T.T., et al. 1989. Mol. Cell. Biol. 9, 1965.
                      Kronstad, J.W., et al. 1989. Gene 79, 97.
                      Perlin, D.S., et al. 1988. J. Biol. Chem. 263, 118.
                      Cullen, D., et al. 1987. Gene 57, 21.
                      Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.
                      Malpartida, F., et al. 1983. Biochem. Biophys. Res. Commun. 117, 6.
                      Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.