400051 | Hygromycin B, Streptomyces sp. - CAS 31282-04-9 - Calbiochem

400051
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical FormulaCAS #
      C₂₀H₃₇N₃O₁₃ 31282-04-9

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      400051-100KU
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          Alu drum 100 ku
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          400051-10MU
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              Alu drum 10 mu
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              400051-1MU
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                  Alu drum 1 mu
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                  400051-5MU
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                      Alu drum 5 mu
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                      Description
                      OverviewUnique aminoglycoside antibiotic that inhibits the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells. Inhibits protein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA. Hph, a gene from E. coli, encodes resistance to hygromycin B and can be isolated and cloned by recombinant DNA techniques. This hygromycin B-resistance gene is particularly useful for identification or selection of recombinant clones in a variety of cell types. Hygromycin B penetrates cells that have been permeabilized by virus infection and can act as an effective antiviral agent.
                      Supplied with a directional insert. Note: 1 KU = 1000 units; 1 MU = 1,000,000 units.
                      Bioassay: 350-450 units/mg Hygromycin B. 106 units is equivalent to ~900 mg.
                      Catalogue Number400051
                      Brand Family Calbiochem®
                      References
                      ReferencesDean, N. 1995. Proc. Natl. Acad. Sci. USA 92, 1287.
                      Hamada, W., et al. 1994. Curr. Genetics 26, 251.
                      Hubbard, S. C., et al. 1994. J. Biol. Chem. 269, 3717.
                      Rikkerink, E. H., et al. 1994. Current Genetics 25, 202.
                      Sugimoto, K., et al. 1994. Plant J. 5, 863.
                      Buchschacher, G.L., Jr., and Panganiban, A.T. 1992. J. Virol. 66, 2731.
                      Gaken, J., et al. 1992. Biotechniques 13, 32.
                      Lama, J., and Carrasco, L. 1992. J. Biol. Chem. 267, 15932.
                      Ma, H., et al. 1992. Gene 117, 161.
                      MacIntyre, G., et al. 1992. Adv. Exp. Med. Biol. 276, 67.
                      Norman, J.A., et al. 1992. Mol. Pharmacol. 41, 53.
                      Bilang, R., et al. 1991. Gene 100, 247.
                      Dale, E., and Ow, D. 1991. Proc. Natl. Acad. Sci. USA 88, 10558.
                      Lee, M.G-S., and Van der Ploeg, L. 1991. Gene 105, 255.
                      Leslie, J.F., and Dickman, M.B. 1991. Applied Environ. Microbiol. 57, 1423.
                      MacIntyre, G., et al. 1991. Antimicrob. Agents Chemother. 35, 2125.
                      Zhou, J., et al. 1991. Gene 107, 307.
                      Bulte, L., and Bennoun, P. 1990. Current Genetics 18, 155.
                      Giordano, T.J., and McAllister, W.T. 1990. Gene 88, 285.
                      Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 24, 1915.
                      Carrasco, L., et al. 1989. Pharmacol. Ther. 9, 311.
                      Crespi, C.L., et al. 1989. Carcinogenesis 10, 295.
                      Damm, B., et al. 1989. Mol. Gen. Genetics 217, 6.
                      Egelhoff, T.T., et al. 1989. Mol. Cell. Biol. 9, 1965.
                      Kronstad, J.W., et al. 1989. Gene 79, 97.
                      Perlin, D.S., et al. 1988. J. Biol. Chem. 263, 118.
                      Cullen, D., et al. 1987. Gene 57, 21.
                      Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.
                      Malpartida, F., et al. 1983. Biochem. Biophys. Res. Commun. 117, 6.
                      Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.
                      Product Information
                      CAS number31282-04-9
                      FormAmber to brown liquid
                      Hill FormulaC₂₀H₃₇N₃O₁₃
                      Chemical formulaC₂₀H₃₇N₃O₁₃
                      Structure formula ImageStructure formula Image
                      Applications
                      Biological Information
                      Biological activityThe activity of Hygromycin B is measured in a zonal inhibition assay using Bacillus subtilis. The activity is measured relative to a reference standard.
                      Primary Targetgrowth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells
                      Secondary targetprotein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA
                      Purity>85% by HPLC
                      Physicochemical Information
                      Dimensions
                      Materials Information
                      Toxicological Information
                      Safety Information according to GHS
                      RTECSWK2130000
                      Safety Information
                      R PhraseR: 26/27/28-37/38-41

                      Very toxic by inhalation, in contact with skin and if swallowed.
                      Irritating to respiratory system and skin.
                      Risk of serious damage to eyes.
                      S PhraseS: 23-26-36/37/39-45

                      Do not breathe fumes.
                      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
                      Wear suitable protective clothing, gloves and eye/face protection.
                      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
                      Product Usage Statements
                      Storage and Shipping Information
                      Ship Code Ambient Temperature Only
                      Toxicity Highly Toxic & Carcinogenic / Teratogenic
                      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
                      Storage +2°C to +8°C
                      Do not freeze Ok to freeze
                      Special InstructionsAqueous stock solutions should be kept at concentrations of ≥50 mg/ml and can be stored at either 4°C or -20°C. Aqueous stock solutions (≥50 mg/ml) are stable for up to 6 months at 4°C or -20°C. Working solutions (< 2 mg/ml) are stable for up to 1 month at 4°C.

                      Note: This product is highly toxic. Read enclosed MSDS before handling.
                      Packaging Information
                      Transport Information
                      Supplemental Information
                      Specifications

                      Documentation

                      SDS

                      Title

                      Safety Data Sheet (SDS) 

                      Certificates of Analysis

                      TitleLot Number
                      400051

                      References

                      Reference overview
                      Dean, N. 1995. Proc. Natl. Acad. Sci. USA 92, 1287.
                      Hamada, W., et al. 1994. Curr. Genetics 26, 251.
                      Hubbard, S. C., et al. 1994. J. Biol. Chem. 269, 3717.
                      Rikkerink, E. H., et al. 1994. Current Genetics 25, 202.
                      Sugimoto, K., et al. 1994. Plant J. 5, 863.
                      Buchschacher, G.L., Jr., and Panganiban, A.T. 1992. J. Virol. 66, 2731.
                      Gaken, J., et al. 1992. Biotechniques 13, 32.
                      Lama, J., and Carrasco, L. 1992. J. Biol. Chem. 267, 15932.
                      Ma, H., et al. 1992. Gene 117, 161.
                      MacIntyre, G., et al. 1992. Adv. Exp. Med. Biol. 276, 67.
                      Norman, J.A., et al. 1992. Mol. Pharmacol. 41, 53.
                      Bilang, R., et al. 1991. Gene 100, 247.
                      Dale, E., and Ow, D. 1991. Proc. Natl. Acad. Sci. USA 88, 10558.
                      Lee, M.G-S., and Van der Ploeg, L. 1991. Gene 105, 255.
                      Leslie, J.F., and Dickman, M.B. 1991. Applied Environ. Microbiol. 57, 1423.
                      MacIntyre, G., et al. 1991. Antimicrob. Agents Chemother. 35, 2125.
                      Zhou, J., et al. 1991. Gene 107, 307.
                      Bulte, L., and Bennoun, P. 1990. Current Genetics 18, 155.
                      Giordano, T.J., and McAllister, W.T. 1990. Gene 88, 285.
                      Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 24, 1915.
                      Carrasco, L., et al. 1989. Pharmacol. Ther. 9, 311.
                      Crespi, C.L., et al. 1989. Carcinogenesis 10, 295.
                      Damm, B., et al. 1989. Mol. Gen. Genetics 217, 6.
                      Egelhoff, T.T., et al. 1989. Mol. Cell. Biol. 9, 1965.
                      Kronstad, J.W., et al. 1989. Gene 79, 97.
                      Perlin, D.S., et al. 1988. J. Biol. Chem. 263, 118.
                      Cullen, D., et al. 1987. Gene 57, 21.
                      Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.
                      Malpartida, F., et al. 1983. Biochem. Biophys. Res. Commun. 117, 6.
                      Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.

                      Brochure

                      Title
                      Antibiotics Profiler
                      Bulk Product Guide
                      Drugs for Selection of Genetic Markers Technical Bulletin
                      G418/Hygromycin B Flyer
                      G418/Hygromycin B Flyer
                      Hygromycin B Technical Bulletin
                      Data Sheet

                      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

                      Revision22-January-2018 JSW
                      DescriptionHygromycin B, an aminoglycoside antibiotic, inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Specifically, it inhibits protein synthesis by interfering with translocation of the 70S ribosome and inducing misreading of the mRNA template (Dean, N., Gonzalez, A., et al., Rao, S.N., et al.). Hygromycin B has been used to select mutants in a wide variety of cells including bacteria (Bilang, R., et al., Salauze, D., et al.), protozoans (Lee, M.G-S., and Van der Ploeg, L.H.T.), yeast (Perlin, D.S., et al.), fungi (Cullen, D., et al., Kronstad, J.W., et al., Egelhoff, T.T., et al., Leslie, J.F., and Dickman, M.B., Bulte, L., and Bennoun, P.), plants (Dean, N., Damm, B., et al., Rikkerink, E. H., et al., Sugimoto, K., et al.), and mammalian cells (Crespi, C.L., et al., Giordano, T.J., and McAllister, W.T., Hubbard, S. C., et al.).

                      Resistance to Hygromycin B is conferred by a gene coding for a phosphotransferase that phosphorylates Hygromycin B, thereby inactivating it (Bilang, R., et al., Malpartida, F., et al.). Hygromycin B is known to selectively penetrate cells that have been rendered permeable by virus infection. This, combined with its potency in inhibiting translation, makes it an effective antiviral agent (MacIntyre, G., et al., Zhou, J., et al.).

                      The analytical data listed below will vary from lot to lot.

                      Bioassay: 350-450 U/mg
                      Concentration: 444,600 U/ml; 394 mg Hygromycin B/ml
                      Density: 1140-1155 mg/ml
                      % solids: 34.6%
                      Fill volumes:
                      100,000 U = 225 µl
                      1,000,000 U = 2.25 ml
                      10,000,000 U = 22.5 ml
                      250,000 U = 562 µl
                      5,000,000 U = 11.25 ml
                      FormAmber to brown liquid
                      Recommended reaction conditions
                      Hygromycin B is not provided under sterile conditions. If a sterile solution is desired, working solutions of Hygromycin B in aqueous media can be passed through a 0.2 µm filter before use. When using this product for mammalian cell selection the working concentration is generally between 50 µg/ml and 1 mg/ml. The appropriate concentration for use must be determined empirically for each cell type. For researchers interested in understanding how the concentration in mg/ml can be calculated from the activity in U/ml we provide the following example as a guideline. For the 1,000,000 Unit size: 1,000,000 Units at 438,440 U/ml

                      Figure 1: Calculations

                      CAS number31282-04-9
                      RTECSWK2130000
                      Chemical formulaC₂₀H₃₇N₃O₁₃
                      Structure formulaStructure formula
                      Purity>85% by HPLC
                      Biological activityThe activity of Hygromycin B is measured in a zonal inhibition assay using Bacillus subtilis. The activity is measured relative to a reference standard.
                      Storage +2°C to +8°C
                      Do Not Freeze Ok to freeze
                      Special InstructionsAqueous stock solutions should be kept at concentrations of ≥50 mg/ml and can be stored at either 4°C or -20°C. Aqueous stock solutions (≥50 mg/ml) are stable for up to 6 months at 4°C or -20°C. Working solutions (< 2 mg/ml) are stable for up to 1 month at 4°C.

                      Note: This product is highly toxic. Read enclosed MSDS before handling.
                      Toxicity Highly Toxic & Carcinogenic / Teratogenic
                      Merck USA index14, 4852
                      ReferencesDean, N. 1995. Proc. Natl. Acad. Sci. USA 92, 1287.
                      Hamada, W., et al. 1994. Curr. Genetics 26, 251.
                      Hubbard, S. C., et al. 1994. J. Biol. Chem. 269, 3717.
                      Rikkerink, E. H., et al. 1994. Current Genetics 25, 202.
                      Sugimoto, K., et al. 1994. Plant J. 5, 863.
                      Buchschacher, G.L., Jr., and Panganiban, A.T. 1992. J. Virol. 66, 2731.
                      Gaken, J., et al. 1992. Biotechniques 13, 32.
                      Lama, J., and Carrasco, L. 1992. J. Biol. Chem. 267, 15932.
                      Ma, H., et al. 1992. Gene 117, 161.
                      MacIntyre, G., et al. 1992. Adv. Exp. Med. Biol. 276, 67.
                      Norman, J.A., et al. 1992. Mol. Pharmacol. 41, 53.
                      Bilang, R., et al. 1991. Gene 100, 247.
                      Dale, E., and Ow, D. 1991. Proc. Natl. Acad. Sci. USA 88, 10558.
                      Lee, M.G-S., and Van der Ploeg, L. 1991. Gene 105, 255.
                      Leslie, J.F., and Dickman, M.B. 1991. Applied Environ. Microbiol. 57, 1423.
                      MacIntyre, G., et al. 1991. Antimicrob. Agents Chemother. 35, 2125.
                      Zhou, J., et al. 1991. Gene 107, 307.
                      Bulte, L., and Bennoun, P. 1990. Current Genetics 18, 155.
                      Giordano, T.J., and McAllister, W.T. 1990. Gene 88, 285.
                      Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 24, 1915.
                      Carrasco, L., et al. 1989. Pharmacol. Ther. 9, 311.
                      Crespi, C.L., et al. 1989. Carcinogenesis 10, 295.
                      Damm, B., et al. 1989. Mol. Gen. Genetics 217, 6.
                      Egelhoff, T.T., et al. 1989. Mol. Cell. Biol. 9, 1965.
                      Kronstad, J.W., et al. 1989. Gene 79, 97.
                      Perlin, D.S., et al. 1988. J. Biol. Chem. 263, 118.
                      Cullen, D., et al. 1987. Gene 57, 21.
                      Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.
                      Malpartida, F., et al. 1983. Biochem. Biophys. Res. Commun. 117, 6.
                      Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.

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                      Categories

                      Life Science Research > Inhibitors and Biochemicals > Small Molecules & Inhibitors > Other Inhibitors of Biological Interest > Protein Synthesis Inhibitors
                      Life Science Research > Inhibitors and Biochemicals > Biochemicals > Antibiotics > Antimicrobial Agents
                      Life Science Research > Cell Culture and Systems > Antibiotics & Selection Agents