S4040 | Hybrisol® I Hybridization Solution, (50% Formamide, 6X SSC)

S4040
500 mL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberS4040
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionHybrisol® I Hybridization Solution, (50% Formamide, 6X SSC)
      OverviewHybrisol® Hybridization Solutions are pre-made, ready-to-use solutions optimized for sensitive single and multiple gene copy detection using membrane-based hybridization techniques. They are designed to give maximum signal and clean background for hybridizations performed on nylon and nitrocellulose membranes. Hybrisol® Solution contains 10% Dextran Sulfate, 1% SDS, 50% Formamide, 6X SSC, Sheared DNA, and Modified Denhardt's Solution.
      References
      Product Information
      PresentationLiquid Hybrisol I ® Solution contains 10% Dextran Sulfate, 1% SDS, 50% Formamide, 6X SSC, Sheared DNA, and Modified

      Denhardt's Solution.
      Applications
      Key Applications
      • Northern Blotting
      • Southern Blotting
      Application NotesBefore using, preheat at 37°C to 45°C for 10 - 20 minutes to dissolve precipitants and mix thoroughly. Use 1 ml for every 15 cm2 membrane for prehybridization and hybridization. Prehybridize for 60 minutes at 42°C to 45°C. Hybridize overnight at 45°C to 52°C. Prehybridization and hybridization temperatures may have to be determined empirically. Refer to the Nomograph located under the "Protocols" link at S4040 product specific page at www.chemicon.com for hybridization temperatures with various stringencies guidelines.
      Biological Information
      Physicochemical Information
      Dimensions
      Volume500 mL
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at room temperature tightly sealed when not in use. Solution can be stored refrigerated however it should be warmed to at least room temperature prior to use dilution for best results. Precipitates may form when the solution is cooled.
      Packaging Information
      Material Size500 mL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      HYBRISOL#174; I HYBRIDIZATION SOLUTION - 2190623 2190623
      HYBRISOL® I HYBRIDIZATION SOLUTION 3030300
      HYBRISOL® I HYBRIDIZATION SOLUTION 2464589
      HYBRISOL® I HYBRIDIZATION SOLUTION 3113113
      HYBRISOL® I HYBRIDIZATION SOLUTION 3046760
      HYBRISOL® I HYBRIDIZATION SOLUTION - 2388863 2388863
      HYBRISOL® I HYBRIDIZATION SOLUTION - 2279542 2279542
      HYBRISOL® I HYBRIDIZATION SOLUTION - 2905361 2905361
      HYBRISOL® I HYBRIDIZATION SOLUTION -2571164 2571164
      HYBRISOL® I HYBRIDIZATION SOLUTION -2586470 2586470

      References

      Reference overviewPub Med ID
      A general method for the isolation of RNA complementary to DNA.
      BOLTON, E T and McCARTHY, B J
      Proc. Natl. Acad. Sci. U.S.A., 48: 1390-7 (1962)  1962

      13870855 13870855