375230 | HGF, Mouse, Recombinant

375230
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      Overview

      Replacement Information

      Key Specifications Table

      Purity
      ≥95% by SDS-PAGE as seen by a 60 kDa (α chain) and a 33 kDa (β chain) bands under reducing conditions.

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      375230-25UG
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      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service

          Glass bottle 25 μg
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          Description
          OverviewRecombinant, mouse hepatocyte growth factor (HGF) consisting of disulfide-linked heterodimeric α (Gln33 to Arg495) and β (Val496 to Leu728) chains expressed in murine myeloma NS0 cell line.
          Catalogue Number375230
          Brand Family Calbiochem®
          SynonymsHepatopoietin A
          References
          ReferencesCorso, S., et al. 2005. Trends Mol. Med. 11, 284.
          Karihaloo, A., et al. 2005. Nephron Exp. Nephrol. 100, e40.
          Urbanek, K., et al. 2005. Circ. Res. 97, 663.
          Hammond, D.E., et al. 2004. Curr. Top. Microbiol. Immunol. 286, 21.
          Rosario, M. and W. Birchmeier. 2004. Dev. Cell 7, 3.
          Gheradi, E., et al. 2003. Proc. Natl. Acad. Sci. 100, 12039.
          Chiu, S.J., et al. 2002. J. Biomed. Sci. 9, 261.
          Tanaka, Y., et al. 2002. Biochem. Biophys. Res. Commun. 299, 472.
          Maeshima, A., et al. 2000. Kid. Int. 58, 1511.
          Lesk, A.M. and W.D. Fordham. 1996. J. Mol. Biol. 258, 501.
          Saelman, E.U.M., et al. 1995. J. Cell Sci. 108, 3531.
          Mineo, R., et al. 1994. Endocrinology 145, 4355.
          Mizuno, K., et al. 1994. J. Biol. Chem. 269, 1131.
          Sasaki, M., et al. 1994. Biochem. Biophys. Res. Commun. 199, 772.
          Montesano, R., et al. 1991. Cell 67, 901.
          Product Information
          FormSolid
          FormulationLyophilized from 0.2 µm sterile-filtered PBS, BSA.
          Applications
          ApplicationHGF, Mouse, Recombinant Recombinant, consists of disulfide-linked heterodimeric α (Gln33 to Arg495) and β (Val496 to Leu728) chains expressed in murine myeloma NS0 cell line.
          Biological Information
          Biological activityCell Proliferation:
          ED₅₀ = 10-30 ng/ml as measured in a cell proliferation assay using IMCD-3 mouse epithelial cells.

          Functional ELISA
          KD <0.2 nM as measured by binding to C-Met (HGF Receptor).
          Purity≥95% by SDS-PAGE as seen by a 60 kDa (α chain) and a 33 kDa (β chain) bands under reducing conditions.
          Physicochemical Information
          ContaminantsEndotoxin: <1.0 EU/µg protein
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          375230

          References

          Reference overview
          Corso, S., et al. 2005. Trends Mol. Med. 11, 284.
          Karihaloo, A., et al. 2005. Nephron Exp. Nephrol. 100, e40.
          Urbanek, K., et al. 2005. Circ. Res. 97, 663.
          Hammond, D.E., et al. 2004. Curr. Top. Microbiol. Immunol. 286, 21.
          Rosario, M. and W. Birchmeier. 2004. Dev. Cell 7, 3.
          Gheradi, E., et al. 2003. Proc. Natl. Acad. Sci. 100, 12039.
          Chiu, S.J., et al. 2002. J. Biomed. Sci. 9, 261.
          Tanaka, Y., et al. 2002. Biochem. Biophys. Res. Commun. 299, 472.
          Maeshima, A., et al. 2000. Kid. Int. 58, 1511.
          Lesk, A.M. and W.D. Fordham. 1996. J. Mol. Biol. 258, 501.
          Saelman, E.U.M., et al. 1995. J. Cell Sci. 108, 3531.
          Mineo, R., et al. 1994. Endocrinology 145, 4355.
          Mizuno, K., et al. 1994. J. Biol. Chem. 269, 1131.
          Sasaki, M., et al. 1994. Biochem. Biophys. Res. Commun. 199, 772.
          Montesano, R., et al. 1991. Cell 67, 901.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision21-June-2013 JSW
          SynonymsHepatopoietin A
          DescriptionRecombinant, mouse hepatocyte growth factor (HGF) consisting of disulfide-linked heterodimeric α (Gln33 to Arg495) and β (Val496 to Leu728) chains expressed in murine myeloma NS0 cell line.
          FormSolid
          FormulationLyophilized from 0.2 µm sterile-filtered PBS, BSA.
          Purity≥95% by SDS-PAGE as seen by a 60 kDa (α chain) and a 33 kDa (β chain) bands under reducing conditions.
          ContaminantsEndotoxin: <1.0 EU/µg protein
          Biological activityCell Proliferation:
          ED₅₀ = 10-30 ng/ml as measured in a cell proliferation assay using IMCD-3 mouse epithelial cells.

          Functional ELISA
          KD <0.2 nM as measured by binding to C-Met (HGF Receptor).
          SolubilityReconstitute to 10 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
          Toxicity Standard Handling
          ReferencesCorso, S., et al. 2005. Trends Mol. Med. 11, 284.
          Karihaloo, A., et al. 2005. Nephron Exp. Nephrol. 100, e40.
          Urbanek, K., et al. 2005. Circ. Res. 97, 663.
          Hammond, D.E., et al. 2004. Curr. Top. Microbiol. Immunol. 286, 21.
          Rosario, M. and W. Birchmeier. 2004. Dev. Cell 7, 3.
          Gheradi, E., et al. 2003. Proc. Natl. Acad. Sci. 100, 12039.
          Chiu, S.J., et al. 2002. J. Biomed. Sci. 9, 261.
          Tanaka, Y., et al. 2002. Biochem. Biophys. Res. Commun. 299, 472.
          Maeshima, A., et al. 2000. Kid. Int. 58, 1511.
          Lesk, A.M. and W.D. Fordham. 1996. J. Mol. Biol. 258, 501.
          Saelman, E.U.M., et al. 1995. J. Cell Sci. 108, 3531.
          Mineo, R., et al. 1994. Endocrinology 145, 4355.
          Mizuno, K., et al. 1994. J. Biol. Chem. 269, 1131.
          Sasaki, M., et al. 1994. Biochem. Biophys. Res. Commun. 199, 772.
          Montesano, R., et al. 1991. Cell 67, 901.