4500-0455 Guava Nexin Reagent for Flow Cytometry - 500 tests

4500-0455
500 Tests  
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      Overview

      Replacement Information
      Description
      Catalogue Number4500-0455
      Trade Name
      • Guava
      DescriptionGuava Nexin Reagent for Flow Cytometry - 500 tests
      OverviewThe Guava Nexin® Annexin V Assay offers a simple, reproducible, and reliable assessment of early apoptosis. The assay relies on the translocation of phosphatidyl serine (PS) to the outer surface of the cell membrane, an event often associated with the onset of apoptosis.
      Simple, sensitive, and reproducible
      A mix-and-read assay for monitoring externalization of PS through the binding of Annexin V to the exposed PS, the automated single-cell analysis assay is the choice for monitoring apoptosis due to its sensitivity and reproducibility. Annexin V is a calcium-dependent phospholipid binding protein with high affinity for phosphatidylserine (PS), a membrane component normally localized to the internal face of the cell membrane. Early in the apoptotic pathway, molecules of PS are translocated to the outer surface of the cell membrane where Annexin V can readily bind to them.
      Two-dye strategy
      The assay relies on a two-dye stategy: 1) Annexin V-PE to detect PS on the external membrane of apoptotic cells and 2) 7-AAD, a cell impermeant dye, as an indicator of membrane structural integrity. 7-AAD is excluded from live, healthy cells and early apoptotic cells, but permeates late-stage apoptotic and dead cells. For use with all instruments. Click here for more information regarding Instrument Compatibility
      References
      Product Information
      Components
      • Annexin V-PE
      • 7-AAD
      • Robust Protocol
      Instrument compatibility
      • EasyCyte
      • EasyCyte Plus
      • EasyCyte Mini
      • PCA
      • PCA-96
      Applications
      ApplicationThe Guava Nexin Annexin V Assay offers a simple, reproducible & reliable assessment of early apoptosis.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size500 Tests
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Brochure

      Title
      Evading Apoptosis: Cell Based Assays
      Guava Cell Health Profiling
      Guava Nexin Annexin V Binding Assay for monitoring induction of apoptosis

      Technical Info

      Title
      A multiplex assay approach to screening cytoactive compounds for cell cycle disruption and apoptotic activity on the Guava EasyCyte System
      Application Note: Benchtop flow cytometry facilitates discrimination between induction of DNA damage, oxidative stress and protein misfolding using a novel multicell GFP reporter assay
      Monitoring apoptosis using four different assays on the Guava PCA System
      Multiplex Assay Approach to Screening Cytoactive Compounds for Cell Cycle Disruption and Apoptotic Activity on the Guava EasyCyte Plus System
      Robustness and Reliability of the Guava Nexin Annexin V Apoptosis Screening Assay on the Guava PCA-96 System
      Screening compounds for apoptotic and cytotoxic activity with the Guava PCA-96 Microplate-based Benchtop Cell Analysis System

      User Guides

      Title
      Guava Nexin Reagent