4500-0540 Guava Caspase 3/7 FAM Kit for Flow Cytometry

4500-0540
100 Tests  
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      Overview

      Replacement Information
      Description
      Catalogue Number4500-0540
      Trade Name
      • Guava
      DescriptionGuava Caspase 3/7 FAM Kit for Flow Cytometry
      OverviewThe Guava Caspase Assay Suite of fluorescent-based assays for Caspase
      3/7, 8, 9 and multi-caspase employ the same protocol making it easier
      to perform multiple caspase assays in parallel. Further, Guava caspase
      reagents are membrane permeable allowing one to measure intracellular
      levels of the enzyme without using harsh lysis methods. The Guava
      Caspase Assays employ a FLICA (Fluorescent Labeled Inhibitor of
      Caspase) reagent that specifically identifies active caspases. Each FLICA is
      comprised of three subunits:
      – an active site binding peptide to confer specifi city (DEVD for caspase 3/7,
      LETD for caspase 8, LEHD for caspase 9, and VAD for multi-caspase)
      – a covalent crosslinking agent (FMK)
      – a green (FAM) or orange-red fl uorescent probe (SR): SR is excited by blue
      or green lasers (PCA Systems or EasyCyte Systems), FAM by blue lasers
      only (EasyCyte Systems)
      The Guava Caspase Assays also include the nuclear DNA stain, 7-AAD, to
      simultaneously evaluate membrane integrity and cell viability.
      References
      Product Information
      Components
      • Caspase 3/7 FAM reagent.
      • 7AAD nuclear DNA to simultaneously evaluate membrane integrity and cell viability.
      Instrument compatibility
      • EasyCyte
      • EasyCyte Mini
      • EasyCyte Plus
      Applications
      ApplicationThe Guava Caspase 3/7 FAM Assay employs the same protocol as other Guava Caspase fluorescent-based assays making it easier to perform multiple caspase assays in parallel.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2-8°C
      Packaging Information
      Material Size100 Tests
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Brochure

      Title
      Guava Caspase 3/7 & Caspase 8 Assay for detection of apoptosis
      Guava Cell Health Profiling
      Innovative Solutions for Flow Cytometry Analysis (EMD)

      Technical Info

      Title
      Application Note: Benchtop flow cytometry facilitates discrimination between induction of DNA damage, oxidative stress and protein misfolding using a novel multicell GFP reporter assay

      Posters

      Title
      A multiplex fluorescence assay approach for determination of apoptosis, mitotic state, and cell viability on a microplate-based single cell analysis system

      User Guides

      Title
      Guava Caspase 3/7

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      Categories

      Life Science Research > Cell Analysis > guava easyCyte Flow Cytometers > Flow Cytometry Kits & Reagents