70967 GeneJuice® Transfection Reagent

70967
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      Overview

      Replacement Information

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      70967-3
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          Glass bottle 1 ml
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          70967-4
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              Glass bottle 10 ml
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              70967-5
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                  Plastic ampoule 0.3 ml
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                  70967-6
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                      Glass bottle 5 ml
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                      Cell lines transfected with GeneJuice® Transfection Reagent
                      Description
                      Overview""I use GeneJuice because it's ease-of-use, low toxicity and wide range of target cells""
                      Stuart Rulten. Research Fellow. University of Sussex

                      ""Since we have been working with GeneJuice, we have saved a lot of time and money!""
                      Dr. Andrea Kress, Institute of Clinical and Molecular Virology, Erlangen, Germany

                      ""I find that Genejuice works for a multitude of cell types with minimal optimization""
                      Caitriona Marie Lyons. PhD Student. University College Cork, Ireland

                      Request a Free Sample

                      Transfection is the process by which nucleic acids are introduced into mammalian cells. GeneJuice Transfection Reagent is a proprietary formulation optimized for maximal transfection efficiency, ease of use, and minimal cytotoxicity for mammalian cells. Whereas many available transfection reagents are based on cationic lipid formulation, GeneJuice Transfection Reagent is composed of a nontoxic cellular protein and a small amount of a novel polyamine. GeneJuice Transfection Reagent enables highly efficient DNA transfer in both stable and transient transfections of eukaryotic cells and is ideal for high-throughput transfections in a multi-well plate format. The unique composition is compatible with both serum-containing and serum-free media, making media changes unnecessary during transfection experiments. Genejuice is a superior alternative to a wide variety of other techniques including calcium phosphate coprecipitation, electroporation, microinjection, biolistic particle delivery, lipofection, and complex formation with DEAE-dextran. The 1 ml size provides enough reagent to perform up to 500 transfections in standard 35 mm plates.
                      Cell Lines:Primary Cells:
                      10T1/2
                      293T
                      3T3 NIH
                      3T3 Swiss
                      3T3-L1
                      A204
                      A431
                      A549
                      alpha TC1-6
                      AR 42J
                      As4.1
                      AtT-20
                      B50
                      BC-1
                      BC-2
                      BC3
                      BCBL
                      BHK-21
                      C3H/10T1/2
                      C6
                      C2C12
                      Caco-2
                      Caki-1
                      Calpan-1
                      Calu-1
                      Calu-6
                      CCL-131
                      CFPAC-1
                      Chang Liver
                      CHO
                      CHO-7
                      CHO-IR
                      CHO-K1
                      COS-1
                      COS-7
                      CS-1
                      CV-1
                      Daudi
                      DDTI MF-2
                      DT40
                      ECV304
                      EL4
                      ES-E14TG2a
                      EVSCC17M
                      H9c2
                      HCT-116
                      HEK293
                      HeLa
                      HeLa B
                      HeLa T4
                      Hep 3B2.1-7
                      HepG2
                      Hepa 1-6
                      Ht-29
                      HTB-37
                      HTB-45
                      Huh-7
                      HUVEC
                      IC21
                      IEC-6
                      JEG-3
                      Jurkat
                      KB
                      L57-3-11
                      L-6
                      L-929
                      MA-10
                      McA-RH7777
                      MCF-7
                      MCF-10-2A
                      MDCK
                      Melanocyte
                      MG-63
                      Neuro 2A
                      Neuroblastoma
                      NRK
                      NT2/D1
                      OV-1063
                      OVCAR3
                      P4
                      P19
                      PC12
                      PA317
                      PAM212
                      PS-1
                      R2C
                      RAW 264.7
                      RBL-2H3
                      RMP-41
                      SAOS-2
                      SC-1
                      Schneider line2
                      SK-N-MC
                      SK-N-SH
                      SKOV3
                      STO
                      SW-480
                      SW-837
                      T3M4
                      TM4
                      U937
                      UCD
                      Vero
                      Aortic smooth muscle cells
                      Astrocytes
                      Angioblasts
                      Chondrocytes
                      Chromaffin cells
                      Epithelial cells:
                        mammary
                        prostate
                        tracheal
                      Fibroblasts
                      Keratinocytes
                      Catalogue Number70967 Brand Family Novagen®
                      Features and benefits
                      • Highly efficient DNA transfer for both stable and transient transfections
                      • Minimal cellular toxicity
                      • Compatibility with both serum-containing and serum-free media
                      • Simple protocol—no need for media changes
                      • Ideal for high-throughput transfection in a multi-well plate format


                      References
                      References
                    • Koleva, R.I., et al. 2006. J. Biol. Chem. 281, 25110.
                    • Product Information
                      DeclarationGeneJuice® is a registered trademark of EMD Chemicals Inc.
                      Applications
                      Biological Information
                      Physicochemical Information
                      Dimensions
                      Materials Information
                      Toxicological Information
                      Safety Information according to GHS
                      Safety Information
                      R PhraseR: 11

                      Highly flammable.
                      S PhraseS: 7-16

                      Keep container tightly closed.
                      Keep away from sources of ignition - No Smoking.
                      Product Usage Statements
                      Storage and Shipping Information
                      Ship Code Blue Ice Only
                      Toxicity Flammable
                      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
                      Storage +2°C to +8°C
                      Do not freeze Ok to freeze
                      Packaging Information
                      Transport Information
                      Supplemental Information
                      Specifications

                      Documentation

                      GeneJuice® Transfection Reagent SDS

                      Title

                      Safety Data Sheet (SDS) 

                      GeneJuice® Transfection Reagent Certificates of Analysis

                      TitleLot Number
                      70967

                      References

                      Reference overview
                    • Koleva, R.I., et al. 2006. J. Biol. Chem. 281, 25110.
                    • Brochure

                      Title
                      Nano Juice™ Transfection Kit
                      Bulk packaging
                      The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression (EMD)

                      Citations

                      Title
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                    • User Protocols

                      Title
                      TB289