341631 Fibronectin, Bovine Plasma

341631
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      341631-1MG
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          Plastic ampoule 1 mg
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          341631-5MG
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              Glass bottle 5 mg
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              Description
              OverviewNative fibronectin purified from pooled bovine plasma. Effective agent for promoting attachment of cells to commonly-used culture substrates.
              Catalogue Number341631
              Brand Family Calbiochem®
              References
              Product Information
              CAS number86088-83-7
              FormLiquid
              FormulationIn 150 mM NaCl, 20 mM sodium phosphate buffer, pH 7.3.
              Applications
              Biological Information
              Puritysingle band by SDS-PAGE
              Concentration Label Please refer to vial label for lot-specific concentration
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Dry Ice Only
              Toxicity Standard Handling
              Storage -20°C
              Avoid freeze/thaw Avoid freeze/thaw
              Do not freeze Ok to freeze
              Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
              Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              SDS

              Title

              Safety Data Sheet (SDS) 

              Certificates of Analysis

              TitleLot Number
              341631
              Data Sheet

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision08-December-2010 RFH
              DescriptionNative fibronectin purified from pooled bovine plasma. Effective agent for promoting attachment of cells to commonly-used culture substrates.
              FormLiquid
              FormulationIn 150 mM NaCl, 20 mM sodium phosphate buffer, pH 7.3.
              Concentration Label Please refer to vial label for lot-specific concentration
              Recommended reaction conditions
              Protocol for Coating of Tissue Culture Plates with Fibronectin

              This protocol is provided only as a guideline; optimal conditions should be determined as needed. This procedure is based on the use of 21 cm2 dishes and 1 mg fibronectin, which is a sufficient quantity to coat 10 dishes at 5 µg/cm2.

              1. Thaw the fibronectin by placing the vial in a 37°C water bath until it is completely thawed. Be VERY careful during the thawing process. DO NOT DISTURB OR REMOVE THE VIAL AT ANY TIME DURING THE THAWING PERIOD. If the vial is disturbed or removed prior to complete thawing, the product will gel and be unusable. DO NOT VORTEX. DO NOT SHAKE AFTER THAWING. Mix very gently after thawing.

              2. After thawing, bring the solution to a final volume of 20 ml with sterile, serum-free medium that has been pre-heated to 37°C. This yields a fibronectin solution of 50 µg/ml.

              3. Add 2 ml to each of 10 tissue culture dishes and swirl gently to completely coat the entrie growth surface.

              4. Incubate the dishes at room temperature for ~45 min to permit binding of the fibronectin to the growth surfaces.

              5. Tilt each dish and remove the fibronecting using a sterile pipette. Do not permit the pipette tip to disturb the growth surface.

              6. Add the cell suspension in medium directly to the fibronectin-coated dishes and incubate under conditions appropriate to the cells.
              CAS number86088-83-7
              Puritysingle band by SDS-PAGE
              Storage -20°C
              Avoid freeze/thaw
              Do Not Freeze Ok to freeze
              Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
              Toxicity Standard Handling
              Merck USA index14, 4075