343242 | FLUO 3/AM - CAS 121714-22-5 - Calbiochem

343242
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical FormulaCAS #
      C₅₁H₅₀Cl₂N₂O₂₃ 121714-22-5

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      343242-1MG
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          Glass bottle 1 mg
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          Description
          OverviewMembrane-permeable version of FLUO 3 (Cat. No. 343244). Unlike all other Ca2+ indicators, FLUO 3/AM is non-fluorescent until it is hydrolyzed in the cell by cellular esterases. It is insensitive to Mg2+.
          Catalogue Number343242
          Brand Family Calbiochem®
          Synonyms1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)]-2-(2ʹ-amino-5ʹ-methylphenoxy)ethane-N,N,Nʹ,Nʹ-tetraacetic Acid Pentaacetoxymethyl Ester
          References
          ReferencesCaputo, C., and Bolanos, P. 1994. J. Physiol. (London) 481, 119.
          Schumann, M.A., et al. 1993. J. Biol. Chem. 268, 2134.
          Eberhard, M., and Erne, P. 1989. Biochem. Biophys. Res. Commun. 163, 309.
          Product Information
          CAS number121714-22-5
          ATP CompetitiveN
          FormReddish orange solid
          Hill FormulaC₅₁H₅₀Cl₂N₂O₂₃
          Chemical formulaC₅₁H₅₀Cl₂N₂O₂₃
          ReversibleN
          Structure formula ImageStructure formula Image
          Applications
          Biological Information
          Primary TargetCa2+ indicators
          Purity≥95% by HPLC
          Physicochemical Information
          Cell permeableN
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          343242

          References

          Reference overview
          Caputo, C., and Bolanos, P. 1994. J. Physiol. (London) 481, 119.
          Schumann, M.A., et al. 1993. J. Biol. Chem. 268, 2134.
          Eberhard, M., and Erne, P. 1989. Biochem. Biophys. Res. Commun. 163, 309.

          Brochure

          Title
          Calcium Metabolism and Related Products Brochure
          Fluorescent Calcium Probes Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision13-May-2013 JSW
          Synonyms1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)]-2-(2ʹ-amino-5ʹ-methylphenoxy)ethane-N,N,Nʹ,Nʹ-tetraacetic Acid Pentaacetoxymethyl Ester
          DescriptionMembrane-permeable version of FLUO 3 (Cat. No. 343244). Cleavage of FLUO 3/AM by cytosolic esterases traps the indicator in the cell. FLUO 3 is almost nonfluorescent at resting calcium levels, but fluorescence levels increase 40-fold upon calcium binding. Unlike all other Ca2+ indicators, FLUO 3/AM is nonfluorescent until it is hydrolyzed by the cell. Since its excitation maximum is at 506 nm, this compound can be used in conjunction with caged compounds, which are released by UV light. Its relatively high Kd for Ca2+ facilitates the measurement of higher peaks of Ca2+ transients than is possible with FURA 2. FLUO 3 has an excitation maximum of 506 nm and an emission maximum of 526 nm in the presence of high and low calcium concentrations.
          FormReddish orange solid
          Intert gas (Yes/No) Packaged under inert gas
          Recommended reaction conditionsThe FLUO 3/AM DMSO stock solution should be diluted to 1-5 µM in a physiological buffer. In some cases, use of the non-ionic detergent PLURIONIC® F-127, PROTEIN GRADE® (Cat. No. 540025) at 0.025-0.125% will assist in the dissolution of the dye in aqueous buffers. Cells are normally incubated with the dye for 20-60 min at 20-37°C.
          CAS number121714-22-5
          Chemical formulaC₅₁H₅₀Cl₂N₂O₂₃
          Structure formulaStructure formula
          Purity≥95% by HPLC
          SolubilityDMF and DMSO (5 mM)
          Storage -20°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
          Toxicity Standard Handling
          ReferencesCaputo, C., and Bolanos, P. 1994. J. Physiol. (London) 481, 119.
          Schumann, M.A., et al. 1993. J. Biol. Chem. 268, 2134.
          Eberhard, M., and Erne, P. 1989. Biochem. Biophys. Res. Commun. 163, 309.